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[185777_s_at]

Description Y53F4B.4B /REP_DB=WormBase Gene ID /WP=CE25405 /TR=Q9NAA7 /GB=CAB70101.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 87      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 91      6grp_total > 96      4grp_total > 52      6grp_highest_sub > Cu      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 230
1 2 3 4 range
A 229.2
(3)
P 		151.3
(-3)
P 		218.3
(2.2)
P 		175.9
(-1.1)
P 		78
B 244.9
(0.1)
P 		356.9
(3)
P 		219.2
(-0.6)
P 		126.9
(-3)
P 		230
C 179.7
(0.5)
P 		176.3
(0.2)
P 		208.2
(3)
P 		139.8
(-3)
P 		68
avr 217.9
(2.2) 		228.2
(3) 		215.2
(2) 		147.5
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 214
(2.5)
P 		128.2
(-3)
P 		136.2
(-2.5)
P 		151.4
(-1.5)
P 		195.5
(1.3)
P 		221.4
(3)
P 		93
B 157.4
(-1.7)
P 		131.1
(-2.8)
P 		125.9
(-3)
P 		194.8
(-0.2)
P 		222.1
(0.9)
P 		272.2
(3)
P 		146
C 112.3
(-3)
P 		167.7
(0.1)
P 		119.4
(-2.6)
P 		195.9
(1.7)
P 		164.7
(-0.1)
P 		219.2
(3)
P 		107
avr 161.2
(-1.2) 		142.3
(-2.2) 		127.2
(-3) 		180.7
(-0.1) 		194.1
(0.6) 		237.6
(3) 		110
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 83.5
(3)
P 		32.8
(-2.3)
P 		57.1
(0.3)
P 		25.7
(-3)
P 		58
B 78.1
(2)
P 		88.1
(3)
P 		50.8
(-0.7)
P 		27
(-3)
P 		61
C 59.5
(1.4)
P 		50.7
(0.3)
P 		72.6
(3)
P 		23.6
(-3)
P 		49
avr 73.7
(3) 		57.2
(1) 		60.2
(1.3) 		25.4
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 39.5
(-1)
P 		45.6
(0.3)
P 		29.7
(-3)
P 		38.4
(-1.2)
P 		59
(3)
P 		53.9
(2)
P 		29
B 54.8
(-1)
P 		43.7
(-1.9)
P 		30.6
(-3)
P 		51.6
(-1.2)
P 		90.2
(2)
P 		101.5
(3)
P 		71
C 41.6
(-1.4)
P 		51.2
(-0.1)
P 		30.4
(-3)
P 		39.4
(-1.7)
P 		53.3
(0.2)
P 		73.4
(3)
P 		43
avr 45.3
(-1) 		46.8
(-0.8) 		30.2
(-3) 		43.1
(-1.3) 		67.5
(1.9) 		76.3
(3) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185777_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3081
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.4B NCBI
Target Description Y53F4B.4B /REP_DB=WormBase Gene ID /WP=CE25405 /TR=Q9NAA7 /GB=CAB70101.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064688(11), NM_064687(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064688 NCBI Caenorhabditis elegans Y53F4B.4b (Y53F4B.4) mRNA, complete cds. 11/11 None
NM_064687 NCBI Caenorhabditis elegans Y53F4B.4a (Y53F4B.4) mRNA, complete cds. 11/11 None
SNAP00000043037 ENSEMBL cdna:SNAP chromosome:CEL140:II:14969800:14976788:1 11/11 None
GENEFINDER00000043147 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14969538:14976788:1 11/11 None
Y53F4B.4b ENSEMBL cdna:known chromosome:CEL140:II:14969538:14976879:1 gene:Y53F4B.4 11/11 None
Y53F4B.4a ENSEMBL cdna:known chromosome:CEL140:II:14969792:14976788:1 gene:Y53F4B.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_069539 1/11 Cross Hyb Matching Probes A 
C02F4.3 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14969534-14976785(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y53F4B.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB70101 Hypothetical protein Y53F4B.4b [Caenorhabditis elegans] ref|NP_497089.2| Y53F4B.4b [Caenorhabditis elegans] 0.0
blast CAB70100 Hypothetical protein Y53F4B.4a [Caenorhabditis elegans] ref|NP_497088.2| Y53F4B.4a [Caenorhabditis elegans] 0.0
blast CAE73450 Hypothetical protein CBG20899 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001678 EMBL-EBI Bacterial Sun/eukaryotic nucleolar Nop1/Nop2 1.6E-12
Pfam IPR001678 EMBL-EBI Bacterial Sun/eukaryotic nucleolar Nop1/Nop2 1.6E-12
Pfam IPR001678 EMBL-EBI Bacterial Sun/eukaryotic nucleolar Nop1/Nop2 1.6E-12
Sequence
Target Sequence 
>C. ELEGANS:185777_S_AT
tatgggcgatggatcgtgcggcggaccgtgtagccactatgaagcagctccttgacgcct
cgaaagtcgcaatcgccagctcattttgtggagattttctgaaaactgacgtcactgata
agaaattttcaaaggtcaaattcgcgattgtagaccctccgtgctctggatcaggaattg
ttaagcgaatggatgagattacaggaggaaatgcggagaaggagcgcctcgagaagctta
aaaatttgcaggtgcacaaaaatttggatttattgaaaatgtgctggaaatgctctgaaa
attgttttaaaaaaaaaaccaaaaaattgattgaaaatgatgcaatgatcctaaagcacg
ctctgaagcttcccggcctgaaacgagccgtctactcaacatgctctgtacacgaagaag
aaaatgagcaagttgttgatgaagtgctcctggacacgtatgtccgtcaaaattatgtgc
tcaagaagaatgtgctcccagaatggacatatcgcggactgaagacctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGGGCGATGGATCGTGCGGCGGA 555 653 797 Antisense
GTGTAGCCACTATGAAGCAGCTCCT 26 487 824 Antisense
TTGACGCCTCGAAAGTCGCAATCGC 210 703 848 Antisense
TCGCAATCGCCAGCTCATTTTGTGG 592 599 863 Antisense
TCAAATTCGCGATTGTAGACCCTCC 360 629 932 Antisense
GAGAAGGAGCGCCTCGAGAAGCTTA 659 391 1012 Antisense
GCAATGATCCTAAAGCACGCTCTGA 69 323 1138 Antisense
TTCCCGGCCTGAAACGAGCCGTCTA 143 699 1166 Antisense
GTCTACTCAACATGCTCTGTACACG 280 469 1186 Antisense
TGAAGTGCTCCTGGACACGTATGTC 108 575 1236 Antisense
GACATATCGCGGACTGAAGACCTAT 628 369 1302 Antisense
Sequence Source Affymetrix Proprietary Database