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[185773_at]

Description C28C12.5 /REP_DB=WormBase Gene ID /WP=CE04112 /TR=Q18279 /GB=AAB37548.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 32      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 25      6grp_total > 31      4grp_total > 47      6grp_highest_sub > 03_04      6grp_cgc > 61      6grp_high1_sub > 02_03      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2536
1 2 3 4 range
A 4282.2
(3)
P 		1857.8
(-3)
P 		2111
(-2.4)
P 		2349.7
(-1.8)
P 		2424
B 4153.8
(3)
P 		1746.2
(-3)
P 		2186.9
(-1.9)
P 		2318.2
(-1.6)
P 		2408
C 3723.6
(3)
P 		1883.5
(-3)
P 		2181.5
(-2)
P 		2150.3
(-2.1)
P 		1840
avr 4053.2
(3) 		1829.2
(-3) 		2159.8
(-2.1) 		2272.7
(-1.8) 		2224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3559
1 2 3 4 5 6 range
A 4186.9
(0.5)
P 		5221.5
(2.7)
P 		5354.4
(3)
P 		5209.6
(2.7)
P 		3425.4
(-1.1)
P 		2547.6
(-3)
P 		2807
B 4101.4
(0.8)
P 		5017
(3)
P 		4694.1
(2.2)
P 		4102.1
(0.8)
P 		3662.7
(-0.2)
P 		2499.6
(-3)
P 		2517
C 4387.3
(0.6)
P 		4804.3
(1.3)
P 		5814
(3)
P 		5203.8
(2)
P 		4177.4
(0.2)
P 		2255.2
(-3)
P 		3559
avr 4225.2
(0.8) 		5014.3
(2.4) 		5287.5
(3) 		4838.5
(2.1) 		3755.2
(-0.2) 		2434.1
(-3) 		2853
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1215
1 2 3 4 range
A 1559.1
(3)
P 		403.3
(-2.7)
P 		551.8
(-2)
P 		344
(-3)
P 		1215
B 1324.6
(3)
P 		431.3
(-3)
P 		506.8
(-2.5)
P 		494
(-2.6)
P 		893
C 1232.6
(3)
P 		541.5
(-1.8)
P 		760.3
(-0.3)
P 		362.6
(-3)
P 		870
avr 1372.1
(3) 		458.7
(-2.6) 		606.3
(-1.7) 		400.2
(-3) 		972

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1237
1 2 3 4 5 6 range
A 771.8
(-2.3)
P 		1857
(3)
P 		1166.1
(-0.4)
P 		1321.3
(0.4)
P 		1033.6
(-1)
P 		620.2
(-3)
P 		1237
B 1427.1
(1)
P 		1669.9
(3)
P 		1139.9
(-1.3)
P 		1086.5
(-1.7)
P 		1488.1
(1.5)
P 		932.1
(-3)
P 		738
C 1626.2
(3)
P 		1465.9
(1.9)
P 		1479.8
(2)
P 		1046.5
(-1)
P 		1351.7
(1.1)
P 		755
(-3)
P 		871
avr 1275
(0.4) 		1664.3
(3) 		1261.9
(0.3) 		1151.4
(-0.4) 		1291.1
(0.5) 		769.1
(-3) 		895
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185773_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8849
Sequence Type Exemplar sequence
Representative Public ID C28C12.5 NCBI
Target Description C28C12.5 /REP_DB=WormBase Gene ID /WP=CE04112 /TR=Q18279 /GB=AAB37548.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069046(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069046 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-8) (spp-8) mRNA, complete cds. 11/11 A 
SNAP00000012165 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8481648:8483516:1 11/11 None
GENEFINDER00000012178 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8481648:8484826:1 11/11 None
C28C12.5 ENSEMBL cdna:known chromosome:CEL140:IV:8481646:8483692:1 gene:C28C12.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8481653-8483522(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-8
Ensembl C28C12.5
Entrez Gene 177652 Entrez gene
SwissProt Q18279 EMBL-EBI
WormBase CE04112 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37548 Saposin-like protein family protein 8 [Caenorhabditis elegans] ref|NP_501447.1| SaPosin-like Protein family member (spp-8) [Caenorhabditis elegans] 0.0
blast CAE71552 Hypothetical protein CBG18499 [Caenorhabditis briggsae] 0.0
blast CAE71552 Hypothetical protein CBG18499 [Caenorhabditis briggsae] 1.0E-180
blast AAB37549 Saposin-like protein family protein 10, isoform b [Caenorhabditis elegans] ref|NP_741465.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 3.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 1.9E-8
Sequence
Target Sequence 
>C. ELEGANS:185773_AT
gctcgtgaagctccgaaacaaccagcatctgagatgtggaagtctatgggaatggtgaag
acaagcaacggagaggagcttatgtcttgcttcgagtgcacactttccgctgatgctctt
cttcaggaattcattgataagagacaaggaacagctgatgacatccaaacagttgcctgc
aataagatggttgccaactggacagatggatgcaatgactttgttcatatgtatatgtca
actgtgcttttcttgacttacaatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCGTGAAGCTCCGAAACAACCAG 226 299 643 Antisense
GAAACAACCAGCATCTGAGATGTGG 651 355 657 Antisense
GAGAGGAGCTTATGTCTTGCTTCGA 345 395 713 Antisense
ATGTCTTGCTTCGAGTGCACACTTT 661 47 724 Antisense
GATGCTCTTCTTCAGGAATTCATTG 59 413 754 Antisense
GATGACATCCAAACAGTTGCCTGCA 172 411 799 Antisense
GTTGCCTGCAATAAGATGGTTGCCA 305 439 814 Antisense
GATGGTTGCCAACTGGACAGATGGA 551 407 828 Antisense
GGATGCAATGACTTTGTTCATATGT 632 513 850 Antisense
TATGTATATGTCAACTGTGCTTTTC 206 657 870 Antisense
ACTGTGCTTTTCTTGACTTACAATC 311 97 883 Antisense
Sequence Source Affymetrix Proprietary Database