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[185755_s_at]

Description W02F12.6 /REP_DB=WormBase Gene ID /WP=CE14482 /TR=O45149 /GB=AAC04461.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 87      4grp_highest_8_8 > 35      4grp_high_8_8 > 56      6grp_highest_nonorm > 88      6grp_total > 87      4grp_total > 63      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 172
1 2 3 4 range
A 1089.4
(2.2)
P 		964.4
(-2.1)
P 		1111.8
(3)
P 		940
(-3)
P 		172
B 1091.1
(3)
P 		991.6
(-1.8)
P 		966.9
(-3)
P 		996.6
(-1.6)
P 		124
C 1098.6
(3)
P 		1000.1
(-3)
P 		1083.8
(2.1)
P 		1051.2
(0.1)
P 		98
avr 1093
(3) 		985.4
(-3) 		1054.2
(0.8) 		995.9
(-2.4) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1064
1 2 3 4 5 6 range
A 1439.9
(-1.7)
P 		1288
(-3)
P 		1317.5
(-2.7)
P 		1460.2
(-1.5)
P 		1441.2
(-1.6)
P 		1966.3
(3)
P 		678
B 1172.3
(-1)
P 		1029.5
(-2.1)
P 		902.3
(-3)
P 		1224.3
(-0.6)
P 		1469.7
(1.2)
P 		1712
(3)
P 		810
C 1169.5
(-3)
P 		1184.8
(-2.9)
P 		1379.7
(-1.3)
P 		1317.7
(-1.8)
P 		1392.2
(-1.2)
P 		1907
(3)
P 		738
avr 1260.6
(-2.2) 		1167.4
(-3) 		1199.8
(-2.7) 		1334.1
(-1.6) 		1434.4
(-0.7) 		1861.8
(3) 		694
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 259
1 2 3 4 range
A 396.6
(3)
P 		209.4
(-1.3)
P 		290.6
(0.5)
P 		137.6
(-3)
P 		259
B 348
(3)
P 		244.9
(-1.6)
P 		224
(-2.5)
P 		212.4
(-3)
P 		136
C 363.7
(2.6)
P 		287.5
(0.3)
P 		377.7
(3)
P 		177.3
(-3)
P 		200
avr 369.4
(3) 		247.3
(-0.8) 		297.4
(0.8) 		175.8
(-3) 		194

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 419
1 2 3 4 5 6 range
A 265.4
(-3)
P 		458.1
(2.4)
P 		286.9
(-2.4)
P 		370.3
(-0)
P 		434.9
(1.8)
P 		478.7
(3)
P 		213
B 407.9
(-0.3)
P 		342.7
(-1.2)
P 		219.1
(-3)
P 		324.3
(-1.5)
P 		597.1
(2.4)
P 		638.4
(3)
P 		419
C 433.5
(-0.3)
P 		361.5
(-1.4)
P 		351.2
(-1.6)
P 		265
(-3)
P 		450.5
(-0)
P 		638.4
(3)
P 		373
avr 368.9
(-1.3) 		387.4
(-1) 		285.7
(-3) 		319.9
(-2.3) 		494.2
(1.2) 		585.2
(3) 		300
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185755_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11620
Sequence Type Exemplar sequence
Representative Public ID W02F12.6 NCBI
Target Description W02F12.6 /REP_DB=WormBase Gene ID /WP=CE14482 /TR=O45149 /GB=AAC04461.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072300(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072300 NCBI Caenorhabditis elegans W02F12.6 (W02F12.6) mRNA, complete cds. 11/11 None
SNAP00000016556 ENSEMBL cdna:SNAP chromosome:CEL140:V:6712290:6713079:-1 11/11 None
GENEFINDER00000016559 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6712290:6713079:-1 11/11 None
W02F12.6 ENSEMBL cdna:known chromosome:CEL140:V:6712237:6713079:-1 gene:W02F12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6712290-6712979(-) 92.86 92.86
Public Domain and Genome References
Gene Title
Ensembl W02F12.6
Entrez Gene 179064 Entrez gene
SwissProt O45149 EMBL-EBI
WormBase CE31084 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_504701 W02F12.6 [Caenorhabditis elegans] gb|AAC04461.2| Hypothetical protein W02F12.6 [Caenorhabditis elegans] 1.0E-105
blast NP_504701 W02F12.6 [Caenorhabditis elegans] gb|AAC04461.2| Hypothetical protein W02F12.6 [Caenorhabditis elegans] 1.0E-102
blast CAE71938 Hypothetical protein CBG19002 [Caenorhabditis briggsae] 4.0E-76
blast CAE71938 Hypothetical protein CBG19002 [Caenorhabditis briggsae] 5.0E-76
blast CAE57168 Hypothetical protein CBG00002 [Caenorhabditis briggsae] 2.0E-25
Sequence
Target Sequence 
>C. ELEGANS:185755_S_AT
actacgcagctatcgccgaaatcgaaaagcaaattgccgataatgcggcaaatttcgaaa
agtggaaaaatgctaacgcgttgcaaagaggatctgccgactataatgcaggagtgacac
gattcaccgactgggatcgtgatctacgaacaaggctcgcatctcatcaaggaataaatg
tcgaatcaggcccgaaaagcatcgatgcagtccttggagagttgctggataaagtagatg
gtgctggctttgcacaagcaattcaaattgcaaactccgctgatcctacattttggccaa
cattacaacaggaatttcacaattttaaagcaaatccaccacagcctgtatatcaaatgc
ctcgatctcaatattacccaagttttgcctcgagtccatacggttatgcagctccacaag
ttacatcaaacgttcctgtctatcggcctgttttgccaacaattatcacacttccgaaag
cagtctcacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTACGCAGCTATCGCCGAAATCGA 704 99 56 Antisense
TACGAACAAGGCTCGCATCTCATCA 382 649 200 Antisense
AGCATCGATGCAGTCCTTGGAGAGT 181 79 253 Antisense
TCCGCTGATCCTACATTTTGGCCAA 214 597 331 Antisense
GCAAATCCACCACAGCCTGTATATC 701 317 385 Antisense
ATGCCTCGATCTCAATATTACCCAA 519 41 412 Antisense
TATTACCCAAGTTTTGCCTCGAGTC 194 661 427 Antisense
GAGTCCATACGGTTATGCAGCTCCA 616 399 447 Antisense
ACATCAAACGTTCCTGTCTATCGGC 641 101 478 Antisense
TGTCTATCGGCCTGTTTTGCCAACA 646 557 492 Antisense
TCACACTTCCGAAAGCAGTCTCACC 94 629 521 Antisense
Sequence Source Affymetrix Proprietary Database