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[185753_s_at]

Description Y50E8A.15 /REP_DB=WormBase Gene ID /WP=CE22313 /TR=Q9NAF0 /GB=CAB55054.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 23      4grp_total > 25      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 1.7
(-3)
A 		5.9
(3)
A 		2.2
(-2.3)
A 		3.1
(-1)
A 		4
B 1.8
(-3)
A 		2.3
(-2.4)
A 		6.7
(3)
A 		2
(-2.8)
A 		5
C 4.5
(-2.4)
A 		5.7
(-1.5)
A 		3.7
(-3)
A 		11.7
(3)
A 		8
avr 2.7
(-3) 		4.6
(0.9) 		4.2
(0.1) 		5.6
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 5.1
(2.6)
A 		2.6
(-1.9)
A 		2
(-3)
A 		5.3
(3)
A 		2.8
(-1.5)
A 		4.2
(1)
A 		3
B 3.5
(-0.5)
A 		1.8
(-2.9)
A 		6
(3)
A 		2.6
(-1.7)
A 		1.8
(-2.9)
A 		1.7
(-3)
A 		4
C 4.3
(0.9)
A 		6.1
(3)
A 		2.9
(-0.7)
A 		3.4
(-0.1)
A 		4.6
(1.3)
A 		0.9
(-3)
A 		5
avr 4.3
(3) 		3.5
(0.6) 		3.6
(0.9) 		3.8
(1.5) 		3.1
(-0.6) 		2.3
(-3) 		2
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 0.6
(-1.7)
A 		1.3
(3)
A 		0.6
(-1.7)
A 		0.4
(-3)
A 		1
B 0.6
(-2)
A 		0.6
(-2)
A 		1.6
(3)
A 		0.4
(-3)
A 		1
C 1.5
(-1.3)
A 		1.6
(-0.4)
A 		1.3
(-3)
A 		2
(3)
A 		1
avr 0.9
(-3) 		1.2
(3) 		1.2
(3) 		0.9
(-3) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2
1 2 3 4 5 6 range
A 0.9
(0.3)
A 		0.9
(0.3)
A 		0.4
(-3)
A 		1.3
(3)
A 		0.9
(0.3)
A 		1
(1)
A 		1
B 1.2
(1)
A 		0.6
(-3)
A 		1.5
(3)
A 		0.7
(-2.3)
A 		0.7
(-2.3)
A 		0.6
(-3)
A 		1
C 1.6
(1.9)
A 		1.9
(3)
A 		0.7
(-1.5)
A 		0.7
(-1.5)
A 		1.5
(1.5)
A 		0.3
(-3)
A 		2
avr 1.2
(3) 		1.1
(2) 		0.9
(0) 		0.9
(0) 		1
(1) 		0.6
(-3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185753_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12789
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.15 NCBI
Target Description Y50E8A.15 /REP_DB=WormBase Gene ID /WP=CE22313 /TR=Q9NAF0 /GB=CAB55054.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074242(11), NM_060812(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074242 NCBI Caenorhabditis elegans Y50E8A.15 (Y50E8A.15) mRNA, complete cds. 11/11 A 
NM_060812 NCBI Caenorhabditis elegans F28C12.6 (F28C12.6) mRNA, complete cds. 11/11 A 
SNAP00000008988 ENSEMBL cdna:SNAP chromosome:CEL140:V:14761697:14762308:1 11/11 None
GENEFINDER00000009984 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14761697:14762308:1 11/11 None
F28C12.6 ENSEMBL cdna:known chromosome:CEL140:I:12699119:12700036:1 gene:F28C12.6 11/11 A 
Y50E8A.15 ENSEMBL cdna:known chromosome:CEL140:V:14761697:14762308:1 gene:Y50E8A.15 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017839 6/11 Cross Hyb Matching Probes None
GENEFINDER00000017847 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12699369-12699826(+) 9.48 73.69
Public Domain and Genome References
Gene Title

Ensembl F28C12.6
Y50E8A.15
Entrez Gene 185058 Entrez gene
190114 Entrez gene
SwissProt O17845 EMBL-EBI
Q9NAF0 EMBL-EBI
WormBase CE09753 Wormbase
CE22313 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07601 Hypothetical protein F28C12.6 [Caenorhabditis elegans] ref|NP_493213.1| F28C12.6 [Caenorhabditis elegans] 1.0E-108
blast CAB55054 Hypothetical protein Y50E8A.15 [Caenorhabditis elegans] ref|NP_506643.1| Y50E8A.15 [Caenorhabditis elegans] 1.0E-103
blast AAF36060 Hypothetical protein Y75D11A.5 [Caenorhabditis elegans] ref|NP_508324.1| Y75D11A.5 [Caenorhabditis elegans] 6.0E-39
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493213.1 1 194-216
F28C12.6 1 194-216
Sequence
Target Sequence 
>C. ELEGANS:185753_S_AT
accgacgatgccgatcaagctgagaagagctccgaaaaagaggtgtacgatgaaatcgct
gagatagctgatatcgccaatagcattcgtgctgctattgaaaagataaggaactacgat
caacaatggaaggatctcttcacgagaattccaacggagagagaaggcatggccgactac
aagaaacggctaggcgactacgatgtcgacattcaaaatgcaaacaaatcgttgcaagga
ctcaaactcaaatatcaagagtttattgcaattcataagaagaaagctccacaaccgaca
gattatccaacatttggtggagatgaagcaagtccaaatcaatcatcgtcgtcaaacaca
acggatcatcaaacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCGACGATGCCGATCAAGCTGAGA 435 89 88 Antisense
TGAAATCGCTGAGATAGCTGATATC 63 571 138 Antisense
GCATTCGTGCTGCTATTGAAAAGAT 321 305 170 Antisense
GGATCTCTTCACGAGAATTCCAACG 593 511 219 Antisense
GAAGGCATGGCCGACTACAAGAAAC 20 335 250 Antisense
CAAGAAACGGCTAGGCGACTACGAT 661 183 267 Antisense
TAAGAAGAAAGCTCCACAACCGACA 108 637 363 Antisense
GCTCCACAACCGACAGATTATCCAA 536 297 373 Antisense
GCAAGTCCAAATCAATCATCGTCGT 371 321 415 Antisense
ATCAATCATCGTCGTCAAACACAAC 38 27 425 Antisense
GTCAAACACAACGGATCATCAAACC 571 463 438 Antisense
Sequence Source Affymetrix Proprietary Database