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[185752_at]

Description Y50E8A.15 /REP_DB=WormBase Gene ID /WP=CE22313 /TR=Q9NAF0 /GB=CAB55054.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 84      4grp_total > 12      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 18.8
(-1.4)
P 		6.9
(-3)
A 		48.4
(2.4)
A 		52.8
(3)
A 		46
B 39.7
(3)
A 		32.8
(1.5)
A 		12.2
(-3)
A 		33.3
(1.6)
A 		28
C 54.4
(0.3)
A 		66.9
(3)
A 		38.9
(-3)
A 		45.6
(-1.6)
A 		28
avr 37.6
(-0.5) 		35.5
(-1.7) 		33.2
(-3) 		43.9
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 33.8
(0.8)
A 		33.2
(0.7)
A 		38.8
(2.4)
A 		21.1
(-3)
A 		25.9
(-1.5)
A 		40.9
(3)
A 		20
B 31.4
(2.5)
A 		16.7
(-3)
A 		31.3
(2.5)
A 		32.7
(3)
A 		26.8
(0.8)
A 		28.7
(1.5)
A 		16
C 15.2
(-3)
A 		38.6
(1.3)
A 		21.6
(-1.8)
A 		41.7
(1.8)
A 		48.1
(3)
A 		32.3
(0.1)
A 		33
avr 26.8
(-3) 		29.5
(-0.8) 		30.6
(0.2) 		31.8
(1.2) 		33.6
(2.7) 		34
(3) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 6.8
(-0.1)
P 		1.5
(-3)
A 		12.6
(3)
A 		7.7
(0.4)
A 		11
B 12.7
(3)
A 		8.1
(0.2)
A 		2.8
(-3)
A 		7.1
(-0.4)
A 		10
C 18
(2.4)
A 		19.2
(3)
A 		13.6
(0.1)
A 		7.7
(-3)
A 		12
avr 12.5
(3) 		9.6
(-0.5) 		9.7
(-0.4) 		7.5
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 6.2
(-2.3)
A 		11.8
(3)
A 		8.5
(-0.1)
A 		5.4
(-3)
A 		7.8
(-0.8)
A 		10
(1.3)
A 		6
B 10.9
(3)
A 		5.6
(-3)
A 		7.6
(-0.7)
A 		8.7
(0.5)
A 		10.9
(3)
A 		10.7
(2.8)
A 		5
C 5.6
(-2.9)
A 		11.8
(0.7)
A 		5.5
(-3)
A 		8.4
(-1.3)
A 		15.6
(3)
A 		10.8
(0.1)
A 		10
avr 7.6
(-2.4) 		9.7
(0.6) 		7.2
(-3) 		7.5
(-2.6) 		11.4
(3) 		10.5
(1.7) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185752_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12789
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.15 NCBI
Target Description Y50E8A.15 /REP_DB=WormBase Gene ID /WP=CE22313 /TR=Q9NAF0 /GB=CAB55054.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074242(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074242 NCBI Caenorhabditis elegans Y50E8A.15 (Y50E8A.15) mRNA, complete cds. 11/11 A 
SNAP00000008988 ENSEMBL cdna:SNAP chromosome:CEL140:V:14761697:14762308:1 11/11 None
GENEFINDER00000009984 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14761697:14762308:1 11/11 None
Y50E8A.15 ENSEMBL cdna:known chromosome:CEL140:V:14761697:14762308:1 gene:Y50E8A.15 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017839 5/11 Cross Hyb Matching Probes None
GENEFINDER00000017847 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12699369-12699826(+) 9.48 73.69
Public Domain and Genome References
Gene Title
Ensembl Y50E8A.15
Entrez Gene 190114 Entrez gene
SwissProt Q9NAF0 EMBL-EBI
WormBase CE22313 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB55054 Hypothetical protein Y50E8A.15 [Caenorhabditis elegans] ref|NP_506643.1| Y50E8A.15 [Caenorhabditis elegans] 1.0E-103
blast AAF36060 Hypothetical protein Y75D11A.5 [Caenorhabditis elegans] ref|NP_508324.1| Y75D11A.5 [Caenorhabditis elegans] 6.0E-39
blast CAB07601 Hypothetical protein F28C12.6 [Caenorhabditis elegans] ref|NP_493213.1| F28C12.6 [Caenorhabditis elegans] 8.0E-28
Sequence
Target Sequence 
>C. ELEGANS:185752_AT
atgagttcggtgtttcgttctcacattggcattatccgtcgtaagctgaaattggcaatc
gaggctgcagatgacaccgacgatgccgatcaagctgagaagagctccgaaaaagaggtg
tacgatgaaatcgctgagatagctgatatcgccaatagcattcgtgctgctattgaaaag
ataaggaactacgatcaacaatggaaggatctcttcacgagaattccaacggagagagaa
ggcatggccgactacaagaaacggctaggcgactacgatgtcgacattcaaaatgcaaac
aaatcgttgcaaggactcaaactcaaatatcaagagtttattgcaattcataagaagaaa
gctccacaaccgacagattatccaacatttggtggagatgaagcaagtccaaatcaatca
tcgtcgtcaaacacaacggatcatcaaaccatcaacatggaaccaatcacaattcgtgtt
ccaagtggaactactggcactccttcaattgtcacttctaccatcaattcagtggttcct
ccaaatcaaatctacaacaatactgtttcacaccaaggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAGTTCGGTGTTTCGTTCTCACA 147 47 13 Antisense
GTTTCGTTCTCACATTGGCATTATC 100 451 24 Antisense
TTGGCATTATCCGTCGTAAGCTGAA 544 707 38 Antisense
TTGGCAATCGAGGCTGCAGATGACA 12 709 64 Antisense
TAGGCGACTACGATGTCGACATTCA 453 653 278 Antisense
AAATCGTTGCAAGGACTCAAACTCA 316 119 313 Antisense
AACCAATCACAATTCGTGTTCCAAG 531 147 473 Antisense
CAAGTGGAACTACTGGCACTCCTTC 47 183 494 Antisense
TTGTCACTTCTACCATCAATTCAGT 702 705 521 Antisense
GTGGTTCCTCCAAATCAAATCTACA 573 489 544 Antisense
AACAATACTGTTTCACACCAAGGAC 699 125 568 Antisense
Sequence Source Affymetrix Proprietary Database