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[185749_at]

Description F47C10.4 /REP_DB=WormBase Gene ID /WP=CE10662 /TR=O01564 /GB=AAB52274.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 45      4grp_total > 29      6grp_cgc > 45      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 44.7
(0.2)
A 		16.7
(-2.6)
A 		13.1
(-3)
A 		72.1
(3)
A 		59
B 92.8
(3)
A 		82.9
(2)
A 		35.6
(-3)
A 		59.2
(-0.5)
A 		57
C 81.7
(3)
A 		25.2
(-1.8)
A 		11.3
(-3)
A 		64
(1.5)
A 		70
avr 73.1
(3) 		41.6
(-0.6) 		20
(-3) 		65.1
(2.1) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 14.2
(-2.7)
A 		12.9
(-2.7)
A 		103.9
(3)
A 		11.1
(-2.8)
A 		13.7
(-2.7)
A 		8.7
(-3)
A 		95
B 61.1
(3)
A 		32.9
(-0.6)
A 		13.7
(-3)
A 		14.3
(-2.9)
A 		30.7
(-0.8)
A 		49.4
(1.5)
A 		47
C 36.5
(1.4)
A 		46.5
(3)
A 		13
(-2.3)
A 		9.5
(-2.9)
A 		8.6
(-3)
A 		46.5
(3)
A 		38
avr 37.3
(1.8) 		30.8
(0.6) 		43.5
(3) 		11.6
(-3) 		17.7
(-1.9) 		34.9
(1.4) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 16.3
(3)
A 		3.6
(-2.9)
A 		3.4
(-3)
A 		10.6
(0.3)
A 		13
B 29.6
(3)
A 		20.5
(0.4)
A 		8.2
(-3)
A 		12.6
(-1.8)
A 		21
C 27
(3)
A 		7.3
(-2.1)
A 		3.9
(-3)
A 		10.8
(-1.2)
A 		23
avr 24.3
(3) 		10.5
(-1.3) 		5.2
(-3) 		11.3
(-1.1) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 2.6
(-2.9)
A 		4.6
(-2.3)
A 		22.6
(3)
A 		2.8
(-2.8)
A 		4.1
(-2.4)
A 		2.1
(-3)
A 		21
B 21.3
(3)
A 		11
(-0.4)
A 		3.3
(-3)
A 		3.8
(-2.8)
A 		12.5
(0.1)
A 		18.4
(2)
A 		18
C 13.5
(2.1)
A 		14.2
(2.4)
A 		3.3
(-2.4)
A 		1.9
(-3)
A 		2.8
(-2.6)
A 		15.6
(3)
A 		14
avr 12.5
(3) 		9.9
(1.4) 		9.7
(1.3) 		2.8
(-3) 		6.5
(-0.7) 		12
(2.7) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185749_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15393
Sequence Type Exemplar sequence
Representative Public ID F47C10.4 NCBI
Target Description F47C10.4 /REP_DB=WormBase Gene ID /WP=CE10662 /TR=O01564 /GB=AAB52274.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071665(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071665 NCBI Caenorhabditis elegans F47C10.4 (F47C10.4) mRNA, complete cds. 11/11 None
SNAP00000021958 ENSEMBL cdna:SNAP chromosome:CEL140:V:3856142:3862746:-1 11/11 None
GENEFINDER00000021960 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3860878:3862746:-1 11/11 None
F47C10.4 ENSEMBL cdna:known chromosome:CEL140:V:3860902:3862746:-1 gene:F47C10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3860901-3862746(-) 98.98 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nhr-187
Ensembl F47C10.4
Entrez Gene 185910 Entrez gene
SwissProt O01564 EMBL-EBI
WormBase CE10662 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
3707 steroid hormone receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52274 Hypothetical protein F47C10.4 [Caenorhabditis elegans] ref|NP_504066.1| F47C10.4 [Caenorhabditis elegans] 0.0
blast AAB52273 Hypothetical protein F47C10.8 [Caenorhabditis elegans] ref|NP_504065.1| F47C10.8 [Caenorhabditis elegans] 1.0E-177
blast AAB52274 Hypothetical protein F47C10.4 [Caenorhabditis elegans] ref|NP_504066.1| F47C10.4 [Caenorhabditis elegans] 1.0E-177
blast AAB52272 Hypothetical protein F47C10.7 [Caenorhabditis elegans] ref|NP_504064.1| F47C10.7 [Caenorhabditis elegans] 1.0E-152
blast CAE62413 Hypothetical protein CBG06502 [Caenorhabditis briggsae] 1.0E-131
blast CAE58241 Hypothetical protein CBG01341 [Caenorhabditis briggsae] 5.0E-78
Protein Domains
Database ID Description E-Value
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 1.1E-50
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 1.2E-13
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 4.7E-47
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 2.3E-51
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 8.1E-48
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 5.1E-5
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 4.6E-5
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 3.3E-5
Sequence
Target Sequence 
>C. ELEGANS:185749_AT
ttgtagtcagggatcccagccacgagatgacaagttcggattggtgcttctttggagcat
atactgctgtgaagtttttgcttggactcggttttatgaagaagctgagcactagagata
agtctcttctcctcgccaactattctgccaaggctaccctgttgttcagtgcaattcgaa
ctatgcgagccaaaaacgataaaatgataaagcccgatggaaaagattttttcgtggaat
ttttgtcccaatggtccgacttctcactccactacacaaaccgtatcagaagcctgctag
ttaacaggcttatcgagctgaacatcactaatgaggagtttattttgattacagtattgt
tcttttgtaatcctgctctcgacgacctctccgaaagcgcagtagccgtgctcacagagc
aacaaaaagcctatggctccgcgctcatgcaatattgtaagctctcctatcagcataatg
gatcgtctcgtttcacggaacttctctcactgtgcacagtta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTAGTCAGGGATCCCAGCCACGA 337 705 470 Antisense
TAGAGATAAGTCTCTTCTCCTCGCC 418 653 582 Antisense
AACTATTCTGCCAAGGCTACCCTGT 639 137 607 Antisense
GCTACCCTGTTGTTCAGTGCAATTC 269 303 622 Antisense
GAATTTTTGTCCCAATGGTCCGACT 217 325 706 Antisense
TCACTCCACTACACAAACCGTATCA 145 627 733 Antisense
GTATTGTTCTTTTGTAATCCTGCTC 300 453 823 Antisense
TCCGAAAGCGCAGTAGCCGTGCTCA 662 591 859 Antisense
GCTCCGCGCTCATGCAATATTGTAA 460 299 905 Antisense
ATGGATCGTCTCGTTTCACGGAACT 515 49 947 Antisense
GAACTTCTCTCACTGTGCACAGTTA 102 351 967 Antisense
Sequence Source Affymetrix Proprietary Database