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[185747_s_at]

Description Y45F10D.9 /REP_DB=WormBase Gene ID /WP=CE16647 /TR=O62479 /GB=CAA16384.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 87      4grp_highest_8_8 > 20      4grp_high_8_8 > 31      6grp_highest_nonorm > 93      6grp_total > 87      4grp_total > 24      6grp_highest_sub > In      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 170.3
(-3)
P 		200.9
(1.9)
P 		175.4
(-2.2)
P 		207.7
(3)
P 		37
B 177
(-0.4)
P 		158.2
(-3)
P 		201.5
(3)
P 		167.5
(-1.7)
P 		43
C 165.4
(-3)
P 		167
(-2.9)
P 		214.1
(1.5)
P 		230.3
(3)
P 		65
avr 170.9
(-3) 		175.4
(-2.1) 		197
(2.1) 		201.8
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 931
1 2 3 4 5 6 range
A 154.4
(-3)
P 		167.8
(-2.9)
P 		182.5
(-2.7)
P 		339.3
(-1.3)
P 		483.1
(-0)
P 		821.7
(3)
P 		667
B 226.4
(-2)
P 		146.8
(-2.7)
P 		111.3
(-3)
P 		261.9
(-1.8)
P 		457.8
(-0.1)
P 		836
(3)
P 		725
C 141.5
(-3)
P 		174.4
(-2.8)
P 		224.1
(-2.5)
P 		262.1
(-2.2)
P 		391.1
(-1.3)
P 		1042.7
(3)
P 		901
avr 174.1
(-2.9) 		163
(-3) 		172.6
(-2.9) 		287.8
(-2) 		444
(-0.7) 		900.1
(3) 		737
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 62
(3)
P 		43.6
(-0.5)
P 		45.8
(-0.1)
P 		30.4
(-3)
P 		32
B 56.5
(3)
P 		39.1
(-2)
P 		46.7
(0.2)
P 		35.7
(-3)
P 		21
C 54.8
(-0.3)
P 		48
(-1.5)
P 		74.6
(3)
P 		38.8
(-3)
P 		36
avr 57.8
(3) 		43.6
(-0.7) 		55.7
(2.4) 		35
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 322
1 2 3 4 5 6 range
A 28.5
(-3)
P 		59.7
(-1.9)
P 		39.7
(-2.6)
P 		86.1
(-1)
P 		145.8
(1.1)
P 		200.1
(3)
P 		172
B 78.8
(-1.9)
P 		48.9
(-2.5)
P 		27
(-3)
P 		69.4
(-2.1)
P 		186
(0.4)
P 		311.7
(3)
P 		285
C 52.4
(-3)
P 		53.2
(-3)
P 		57
(-2.9)
P 		52.7
(-3)
P 		126.6
(-1.5)
P 		349
(3)
P 		297
avr 53.2
(-2.7) 		53.9
(-2.7) 		41.2
(-3) 		69.4
(-2.3) 		152.8
(-0.3) 		286.9
(3) 		246
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185747_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8968
Sequence Type Exemplar sequence
Representative Public ID Y45F10D.9 NCBI
Target Description Y45F10D.9 /REP_DB=WormBase Gene ID /WP=CE16647 /TR=O62479 /GB=CAA16384.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070259(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070259 NCBI Caenorhabditis elegans Spindle ASsembly abnormal family member (sas-6) (sas-6) mRNA, complete cds. 11/11 None
SNAP00000025708 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13785759:13788677:-1 11/11 None
GENEFINDER00000025735 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13786971:13788677:-1 11/11 None
Y45F10D.9 ENSEMBL cdna:known chromosome:CEL140:IV:13786914:13788690:-1 gene:Y45F10D.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13786976-13788683(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sas-6
Ensembl Y45F10D.9
Entrez Gene 178345 Entrez gene
SwissProt O62479 EMBL-EBI
WormBase CE16647 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5814 centriole inferred from direct assay QuickGO AmiGO
5737 cytoplasm inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
19904 protein domain specific binding inferred from physical interaction QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA16384 Hypothetical protein Y45F10D.9 [Caenorhabditis elegans] ref|NP_502660.1| Spindle ASsembly abnormal family member (sas-6) [Caenorhabditis elegans] sp|O62479|SAS6_CAEEL Spindle assembly abnormal protein 0.0
blast Q60P76 Spindle assembly abnormal protein 1.0E-138
blast CAE74596 Hypothetical protein CBG22377 [Caenorhabditis briggsae] 1.0E-138
Sequence
Target Sequence 
>C. ELEGANS:185747_S_AT
gaagcttcgagcagaacttggcacggctcaacggaacctggagaaggccgatcagctgtt
gaagcgaaactcccaacaacagaatcaacaaagtctggatatgagaaaactaggagagct
ggaagctgatttgaaggaaaaagattcgatggttgagagtctcactgaaactattggaat
cttgaggaaggagctggagaacgagaaattaaaggccgccgagaatatggatagttttga
gaagctgagcatggagaatgagaatcttaaagaaaaaattgcccattaccgagcccaacg
gtttagtccagctccttctgggcttcccgggcttcagaccggactaacaaataggcttac
accatcattcaagcctgttcttggaccccacactccatatggagctaatttgaactcgcg
caccccattccgtgacaatacaactcttaatttccaaaattcgacaattgcaactcctca
tgcttttcgtttcaacagtcaactaatcgccgacgagactactggttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCTTCGAGCAGAACTTGGCACG 517 341 930 Antisense
GAGAAGGCCGATCAGCTGTTGAAGC 550 389 970 Antisense
GAGCCCAACGGTTTAGTCCAGCTCC 250 385 1220 Antisense
TCCCGGGCTTCAGACCGGACTAACA 375 587 1254 Antisense
TACACCATCATTCAAGCCTGTTCTT 158 643 1287 Antisense
GACCCCACACTCCATATGGAGCTAA 212 381 1313 Antisense
TGGAGCTAATTTGAACTCGCGCACC 444 549 1329 Antisense
CCCCATTCCGTGACAATACAACTCT 336 261 1352 Antisense
AATTCGACAATTGCAACTCCTCATG 532 177 1387 Antisense
TCCTCATGCTTTTCGTTTCAACAGT 218 591 1404 Antisense
TAATCGCCGACGAGACTACTGGTTC 207 635 1433 Antisense
Sequence Source Affymetrix Proprietary Database