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[185738_at]

Description Y50E8A.11 /REP_DB=WormBase Gene ID /WP=CE22318 /TR=Q9NAE7 /GB=CAB55059.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 02      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 3
(1)
A 		3.7
(2.8)
A 		1.4
(-3)
A 		3.8
(3)
A 		2
B 1.8
(-3)
A 		2.9
(-1.2)
A 		4.4
(1.3)
A 		5.4
(3)
A 		4
C 2.4
(-3)
A 		3.3
(-1.1)
A 		2.4
(-3)
A 		5.3
(3)
A 		3
avr 2.4
(-3) 		3.3
(-0.8) 		2.7
(-2.3) 		4.8
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 5.8
(3)
A 		3.9
(0)
A 		3.5
(-0.6)
A 		2
(-3)
A 		2.6
(-2.1)
A 		2.4
(-2.4)
A 		4
B 2.7
(-2.8)
A 		9.4
(3)
A 		3.9
(-1.8)
A 		4.5
(-1.3)
A 		2.5
(-3)
A 		3.1
(-2.5)
A 		7
C 2.4
(-2.2)
A 		6.5
(3)
A 		1.8
(-3)
A 		4.6
(0.6)
A 		2.7
(-1.9)
A 		3.4
(-1)
A 		5
avr 3.6
(-1.5) 		6.6
(3) 		3.1
(-2.3) 		3.7
(-1.4) 		2.6
(-3) 		3
(-2.4) 		4
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1
1 2 3 4 range
A 1.1
(3)
A 		0.8
(0.4)
A 		0.4
(-3)
A 		0.6
(-1.3)
A 		1
B 0.6
(-3)
A 		0.7
(-1.8)
A 		1
(1.8)
A 		1.1
(3)
A 		1
C 0.8
(-3)
A 		0.9
(3)
A 		0.8
(-3)
A 		0.9
(3)
A 		0
avr 0.8
(0) 		0.8
(0) 		0.7
(-3) 		0.9
(3) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3
1 2 3 4 5 6 range
A 1.1
(1)
A 		1.4
(3)
A 		0.8
(-1)
A 		0.5
(-3)
A 		0.8
(-1)
A 		0.6
(-2.3)
A 		1
B 0.9
(-3)
A 		3.1
(3)
A 		0.9
(-3)
A 		1.2
(-2.2)
A 		1
(-2.7)
A 		1.1
(-2.5)
A 		2
C 0.9
(-1.4)
A 		2
(3)
A 		0.5
(-3)
A 		0.9
(-1.4)
A 		0.9
(-1.4)
A 		1.2
(-0.2)
A 		2
avr 1
(-1.8) 		2.2
(3) 		0.7
(-3) 		0.9
(-2.2) 		0.9
(-2.2) 		1
(-1.8) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185738_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13554
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.11 NCBI
Target Description Y50E8A.11 /REP_DB=WormBase Gene ID /WP=CE22318 /TR=Q9NAE7 /GB=CAB55059.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074247(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074247 NCBI Caenorhabditis elegans Y50E8A.11 (Y50E8A.11) mRNA, complete cds. 11/11 A 
SNAP00000008994 ENSEMBL cdna:SNAP chromosome:CEL140:V:14784082:14784798:1 11/11 None
GENEFINDER00000009977 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14784082:14784798:1 11/11 None
Y50E8A.11 ENSEMBL cdna:known chromosome:CEL140:V:14784082:14784798:1 gene:Y50E8A.11 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14784081-14784798(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50E8A.11
Entrez Gene 190111 Entrez gene
SwissProt Q9NAE7 EMBL-EBI
WormBase CE22318 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB55059 Hypothetical protein Y50E8A.11 [Caenorhabditis elegans] ref|NP_506648.1| Y50E8A.11 [Caenorhabditis elegans] 3.0E-94
blast CAA21758 Hypothetical protein Y75B12A.2 [Caenorhabditis elegans] emb|CAB03271.1| Hypothetical protein Y75B12A.2 [Caenorhabditis elegans] ref|NP_506725.1| Y75B12A.2 [Caenorhabditis elegans] 3.0E-82
blast CAB03276 Hypothetical protein AH10.2 [Caenorhabditis elegans] emb|CAB02689.1| Hypothetical protein AH10.2 [Caenorhabditis elegans] ref|NP_506503.1| AH10.2 [Caenorhabditis elegans] 1.0E-34
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506648.1 1 155-177
Y50E8A.11 1 155-177
SNAP00000008994 1 155-177
GENEFINDER00000009977 1 155-177
Sequence
Target Sequence 
>C. ELEGANS:185738_AT
aagtcttcaaactgtggaattggcaatgggagcttcaagaactctcagttttcatccaaa
agatcatcaagagactcttgaaattgtattttcaaaggaattcaacttcccattgaagta
tgcttttaaaatggaaactgatgatagtagctatcaaacatttgcattcactgatattgt
tcttcttgttaaatcgacttttgaatgtgatgctggtttcaatggaaaatcatgtcaaga
gaagactacaagtacaacaactactacttctactacaactgaaatcacaactactaccac
tacagttttgatttcggaagttccatcaactactccaattattaaagttgcagcattcca
acaatcaaatccctcaaatattgttctgtactccgtgcttatcctcgtcatagtcactct
tctcatcttggttttcatagttttcttccttctcaaattcccaaagaaaaacgtctacat
tcgaccagaaactccaaatttggaaaactgcccagtgaagattgaagattctagattctt
ttctcctatgagccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTCTTCAAACTGTGGAATTGGCA 118 157 90 Antisense
ATGGGAGCTTCAAGAACTCTCAGTT 200 51 115 Antisense
TTCCAACAATCAAATCCCTCAAATA 658 697 445 Antisense
AAATATTGTTCTGTACTCCGTGCTT 339 115 465 Antisense
ACTCTTCTCATCTTGGTTTTCATAG 210 101 505 Antisense
CATCTTGGTTTTCATAGTTTTCTTC 325 213 513 Antisense
TTTCTTCCTTCTCAAATTCCCAAAG 366 561 531 Antisense
ACGTCTACATTCGACCAGAAACTCC 343 95 560 Antisense
AAACTGCCCAGTGAAGATTGAAGAT 542 127 594 Antisense
GAAGATTCTAGATTCTTTTCTCCTA 549 337 613 Antisense
TAGATTCTTTTCTCCTATGAGCCCA 429 651 621 Antisense
Sequence Source Affymetrix Proprietary Database