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[185736_at]

Description R12C12.7 /REP_DB=WormBase Gene ID /WP=CE21121 /TR=Q21963 /GB=AAK71391.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 53      4grp_highest_8_8 > 20      4grp_high_8_8 > 31      6grp_highest_nonorm > 76      6grp_total > 53      4grp_total > 32      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 479
1 2 3 4 range
A 945.4
(0.9)
P 		726.3
(-3)
P 		920.9
(0.5)
P 		1063.8
(3)
P 		338
B 1046.4
(-3)
P 		1164
(2.5)
P 		1175.2
(3)
P 		1094.4
(-0.8)
P 		129
C 1000.1
(-3)
P 		1086.1
(-0.5)
P 		1205.6
(3)
P 		1127.5
(0.7)
P 		206
avr 997.3
(-2.7) 		992.1
(-3) 		1100.6
(3) 		1095.2
(2.7) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 868
1 2 3 4 5 6 range
A 790.1
(-3)
P 		1009.3
(-1.5)
P 		1658.4
(3)
P 		1091.1
(-0.9)
P 		909.8
(-2.2)
P 		875.9
(-2.4)
P 		868
B 953.9
(-3)
P 		1437.5
(3)
P 		1327.4
(1.6)
P 		1086.9
(-1.3)
P 		1083.8
(-1.4)
P 		1198.3
(0)
P 		484
C 1025.6
(-0.7)
P 		1259.6
(3)
P 		885.1
(-3)
P 		935.4
(-2.2)
P 		986.9
(-1.4)
P 		1164
(1.5)
P 		375
avr 923.2
(-3) 		1235.5
(2.1) 		1290.3
(3) 		1037.8
(-1.1) 		993.5
(-1.9) 		1079.4
(-0.4) 		367
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 265
1 2 3 4 range
A 344.2
(3)
P 		157.7
(-2.9)
P 		240.7
(-0.3)
P 		155.7
(-3)
P 		189
B 333.7
(3)
P 		287.5
(0.2)
P 		272.3
(-0.7)
P 		233.2
(-3)
P 		101
C 331
(0.7)
P 		312.2
(0.2)
P 		420.2
(3)
P 		190.1
(-3)
P 		230
avr 336.3
(3) 		252.5
(-0.5) 		311.1
(1.9) 		193
(-3) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 333
1 2 3 4 5 6 range
A 145.7
(-3)
P 		358.9
(2.9)
P 		361.2
(3)
P 		276.7
(0.6)
P 		274.5
(0.6)
P 		213.3
(-1.1)
P 		216
B 331.9
(-1.6)
P 		478.5
(3)
P 		322.3
(-1.9)
P 		287.9
(-3)
P 		440.3
(1.8)
P 		446.9
(2)
P 		191
C 380.2
(2.7)
P 		384.3
(2.8)
P 		225.3
(-1.9)
P 		188.1
(-3)
P 		319.4
(0.9)
P 		389.7
(3)
P 		202
avr 285.9
(-1.7) 		407.2
(3) 		302.9
(-1) 		250.9
(-3) 		344.7
(0.6) 		350
(0.8) 		156
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185736_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5546
Sequence Type Exemplar sequence
Representative Public ID R12C12.7 NCBI
Target Description R12C12.7 /REP_DB=WormBase Gene ID /WP=CE21121 /TR=Q21963 /GB=AAK71391.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062809(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062809 NCBI Caenorhabditis elegans R12C12.7 (R12C12.7) mRNA, complete cds. 11/11 None
SNAP00000025499 ENSEMBL cdna:SNAP chromosome:CEL140:II:6055110:6055940:-1 11/11 None
GENEFINDER00000025599 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6055110:6055940:-1 11/11 None
R12C12.7 ENSEMBL cdna:known chromosome:CEL140:II:6055110:6056343:-1 gene:R12C12.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6055106-6056364(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12C12.7
Entrez Gene 187841 Entrez gene
SwissProt Q21963 EMBL-EBI
WormBase CE36885 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495210 R12C12.7 [Caenorhabditis elegans] gb|AAK71391.2| Hypothetical protein R12C12.7 [Caenorhabditis elegans] 4.0E-85
blast T16735 hypothetical protein R12C12.7 - Caenorhabditis elegans 4.0E-85
blast ABA03108 Hypothetical protein Y55F3C.6 [Caenorhabditis elegans] 2.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR009667 EMBL-EBI Protein of unknown function DUF1258 2.3E-7
Sequence
Target Sequence 
>C. ELEGANS:185736_AT
gaatccgaccgttcacaagagatattctgaccgaacttgctcagaaaatcaacaagaact
caaaaaatggaccgggaatgggcgaaatttctgttgttagaatagagatggaacatgagc
aagaaaacctccaatcggagtcggattctacagaatacgaagatgaagaggaatcttatg
atgattcaaaagaaacagaagaaacagaagaggaagacgagattttcgaatccgaaaatg
tagaaatgaatgttagagattcactgtggcgatttctctccgatgtcgatcttcggagaa
tgcatttcttacttactacaactcactcaaaagctgctgtttgtctctatttcgacattg
aatgtattgaaaatgaagaaagcatgaaaaaattgttgcttggtgaaagccatctgaaga
caattgaagcgtgtgcagtttgtgctgaggacttggaaaaatgtaaatgcaaaaaagcac
cgaagaagtgtaaaataacttttatgaacttgcacgagaaaattcaatcatttcttcgag
cgtacggacaaactattcgtga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCCGACCGTTCACAAGAGATAT 98 331 92 Antisense
GAGATATTCTGACCGAACTTGCTCA 633 395 110 Antisense
AAACCTCCAATCGGAGTCGGATTCT 347 125 216 Antisense
TAGAGATTCACTGTGGCGATTTCTC 484 651 345 Antisense
ATTTCTCTCCGATGTCGATCTTCGG 574 15 363 Antisense
ATGCATTTCTTACTTACTACAACTC 269 43 391 Antisense
ACTACAACTCACTCAAAAGCTGCTG 698 99 406 Antisense
AGCTGCTGTTTGTCTCTATTTCGAC 146 81 423 Antisense
GAAGCGTGTGCAGTTTGTGCTGAGG 658 343 517 Antisense
TTCAATCATTTCTTCGAGCGTACGG 563 683 614 Antisense
GAGCGTACGGACAAACTATTCGTGA 48 387 629 Antisense
Sequence Source Affymetrix Proprietary Database