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[185720_at]

Description H22K11.3 /REP_DB=WormBase Gene ID /WP=CE19497 /TR=O76631 /GB=AAK39241.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 44      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 62      6grp_total > 20      4grp_total > 59      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 224
1 2 3 4 range
A 204.5
(0.8)
P 		253.2
(3)
P 		236.7
(2.3)
P 		118
(-3)
P 		135
B 215.8
(0.2)
P 		319.7
(3)
P 		293.9
(2.3)
P 		95.6
(-3)
P 		224
C 214.7
(0.5)
P 		221.4
(0.8)
P 		268.6
(3)
P 		136.9
(-3)
P 		132
avr 211.7
(0.8) 		264.8
(2.9) 		266.4
(3) 		116.8
(-3) 		150

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 402
1 2 3 4 5 6 range
A 448.2
(-1.4)
P 		654
(3)
P 		570.3
(1.2)
P 		452.7
(-1.3)
P 		371
(-3)
P 		436.1
(-1.6)
P 		283
B 459.7
(0.4)
P 		583.8
(3)
P 		519.3
(1.6)
P 		547.2
(2.2)
P 		297.2
(-3)
P 		367.6
(-1.5)
P 		287
C 251.9
(-3)
P 		610.6
(3)
P 		480.2
(0.8)
P 		470.9
(0.7)
P 		254.5
(-3)
P 		449.3
(0.3)
P 		359
avr 386.6
(-1.5) 		616.1
(3) 		523.3
(1.2) 		490.3
(0.6) 		307.6
(-3) 		417.7
(-0.9) 		309
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 74.4
(3)
P 		55
(1)
P 		61.9
(1.7)
P 		17.3
(-3)
P 		57
B 68.8
(2)
P 		79
(3)
P 		68.1
(1.9)
P 		20.4
(-3)
P 		59
C 71.1
(1.1)
P 		63.7
(0.5)
P 		93.6
(3)
P 		23.1
(-3)
P 		71
avr 71.4
(2.7) 		65.9
(2) 		74.5
(3) 		20.3
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 82.6
(-3)
P 		232.6
(3)
P 		124.2
(-1.3)
P 		114.8
(-1.7)
P 		112
(-1.8)
P 		106.2
(-2.1)
P 		150
B 159.9
(0.2)
P 		194.3
(3)
P 		126.1
(-2.6)
P 		144.9
(-1)
P 		120.7
(-3)
P 		137.1
(-1.7)
P 		74
C 93.4
(-2.4)
P 		186.3
(3)
P 		122.2
(-0.7)
P 		94.7
(-2.3)
P 		82.4
(-3)
P 		150.4
(0.9)
P 		104
avr 112
(-2.6) 		204.4
(3) 		124.2
(-1.8) 		118.1
(-2.2) 		105
(-3) 		131.2
(-1.4) 		99
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185720_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16670
Sequence Type Exemplar sequence
Representative Public ID H22K11.3 NCBI
Target Description H22K11.3 /REP_DB=WormBase Gene ID /WP=CE19497 /TR=O76631 /GB=AAK39241.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076738(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076738 NCBI Caenorhabditis elegans H22K11.3 (H22K11.3) mRNA, complete cds. 9/11 None
SNAP00000015665 ENSEMBL cdna:SNAP chromosome:CEL140:X:6783211:6784150:1 9/11 None
GENEFINDER00000015671 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6783211:6784150:1 10/11 None
H22K11.3 ENSEMBL cdna:known chromosome:CEL140:X:6783211:6784150:1 gene:H22K11.3 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6783210-6784150(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H22K11.3
Entrez Gene 186761 Entrez gene
SwissProt O76631 EMBL-EBI
WormBase CE35732 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39241 Hypothetical protein H22K11.3 [Caenorhabditis elegans] ref|NP_509139.2| H22K11.3 [Caenorhabditis elegans] 2.0E-80
blast AAK39241 Hypothetical protein H22K11.3 [Caenorhabditis elegans] ref|NP_509139.2| H22K11.3 [Caenorhabditis elegans] 1.0E-74
blast CAE65787 Hypothetical protein CBG10886 [Caenorhabditis briggsae] 7.0E-37
blast CAE65787 Hypothetical protein CBG10886 [Caenorhabditis briggsae] 2.0E-35
Sequence
Target Sequence 
>C. ELEGANS:185720_AT
gctacacatgatcggctgaaacgccaaaatggatatggaatgtggaataatggcggtgaa
ggaggcggagctggagaaggaggaaatttcccaatgggcaattggaatggaccaaactcc
aatgtttccggtttaggctacagtggtcctcagcaacctcctccagacaatagtatcaag
ggaagtgtggggaataaaaattcaaagccagcagaagagactgaaaatcagaataaagaa
gatggtcagaccccttggatgccaaacgaagccaatggcccaagtggatataataatggt
cctcacccaaatggaaactttccgaacttgaatgggtataacaacggtcccagttcattc
aatggtggaaacactaatgtagatgacggtattaaagggagtgttggagcagcagttgag
ccaactaagtcaccatacccaaacaatgggtatgggtacggaaaccgaaacggttatgga
aataattttggtttcaatcgtatggccggaggtaatggttatggaaatagttttggaaga
cccattcttccgagaggagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTACACATGATCGGCTGAAACGCC 235 303 124 Antisense
GGCGGTGAAGGAGGCGGAGCTGGAG 79 541 175 Antisense
TGGACCAAACTCCAATGTTTCCGGT 158 547 231 Antisense
GGTTTAGGCTACAGTGGTCCTCAGC 614 505 253 Antisense
CAACCTCCTCCAGACAATAGTATCA 680 185 277 Antisense
AAGATGGTCAGACCCCTTGGATGCC 100 155 362 Antisense
AATGGTCCTCACCCAAATGGAAACT 211 163 418 Antisense
AACAACGGTCCCAGTTCATTCAATG 453 129 463 Antisense
TTGAGCCAACTAAGTCACCATACCC 414 699 539 Antisense
GGTTTCAATCGTATGGCCGGAGGTA 115 505 613 Antisense
AAGACCCATTCTTCCGAGAGGAGAG 651 151 660 Antisense
Sequence Source Affymetrix Proprietary Database