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[185708_s_at]

Description F56A4.10 /REP_DB=WormBase Gene ID /WP=CE17005 /TR=O44595 /GB=AAF39848.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 19      4grp_high_8_8 > 41      6grp_total > 07      4grp_total > 54      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 364
1 2 3 4 range
A 426.7
(3)
P 		188.6
(-1.4)
P 		234.2
(-0.5)
P 		99.2
(-3)
A 		328
B 384.3
(3)
P 		237.6
(0.3)
P 		165
(-1.1)
P 		62.3
(-3)
P 		322
C 422.8
(3)
P 		279
(0.3)
P 		200.1
(-1.2)
P 		108
(-3)
P 		315
avr 411.3
(3) 		235.1
(-0.3) 		199.8
(-0.9) 		89.8
(-3) 		322

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 91.4
(3)
P 		63.4
(0)
P 		71.6
(0.9)
P 		58.3
(-0.5)
M 		35.2
(-3)
A 		71
(0.8)
P 		56
B 80.5
(3)
P 		59.4
(-1.3)
P 		73.1
(1.5)
P 		64.3
(-0.3)
P 		77.9
(2.5)
P 		51.3
(-3)
P 		29
C 79
(3)
P 		77.8
(2.9)
P 		63
(1.2)
P 		72.4
(2.2)
A 		26.3
(-3)
A 		60.3
(0.9)
P 		53
avr 83.6
(3) 		66.9
(0.3) 		69.2
(0.7) 		65
(-0) 		46.5
(-3) 		60.9
(-0.7) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 155.4
(3)
P 		40.9
(-1.9)
P 		61.2
(-1)
P 		14.5
(-3)
A 		141
B 122.6
(3)
P 		58.7
(-0.5)
P 		38.2
(-1.6)
P 		13.3
(-3)
P 		109
C 140
(3)
P 		80.2
(0.1)
P 		69.7
(-0.5)
P 		18.2
(-3)
P 		122
avr 139.3
(3) 		59.9
(-0.8) 		56.4
(-1) 		15.3
(-3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 16.8
(0.1)
P 		22.5
(3)
P 		15.6
(-0.5)
P 		14.8
(-0.9)
M 		10.6
(-3)
A 		17.3
(0.4)
P 		12
B 28
(1.5)
P 		19.8
(-1.9)
P 		17.7
(-2.7)
P 		17
(-3)
P 		31.7
(3)
P 		19.1
(-2.1)
P 		15
C 29.3
(3)
P 		23.7
(1.4)
P 		16
(-0.8)
P 		14.6
(-1.2)
A 		8.5
(-3)
A 		20.2
(0.4)
P 		21
avr 24.7
(3) 		22
(1.2) 		16.4
(-2.4) 		15.5
(-3) 		16.9
(-2.1) 		18.9
(-0.8) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185708_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15376
Sequence Type Exemplar sequence
Representative Public ID F56A4.10 NCBI
Target Description F56A4.10 /REP_DB=WormBase Gene ID /WP=CE17005 /TR=O44595 /GB=AAF39848.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071250(10), NM_071276(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071250 NCBI Caenorhabditis elegans Y19D10A.8 (Y19D10A.8) mRNA, complete cds. 10/11 None
NM_071276 NCBI Caenorhabditis elegans F56A4.10 (F56A4.10) mRNA, complete cds. 11/11 None
SNAP00000029966 ENSEMBL cdna:SNAP chromosome:CEL140:V:2353187:2360429:-1 10/11 None
GENEFINDER00000029980 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2353187:2356446:-1 10/11 None
SNAP00000032947 ENSEMBL cdna:SNAP chromosome:CEL140:V:2461148:2468390:-1 10/11 None
GENEFINDER00000032951 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2461148:2464407:-1 10/11 None
Y19D10A.8 ENSEMBL cdna:known chromosome:CEL140:V:2353187:2356233:-1 gene:Y19D10A.8 10/11 None
F56A4.10 ENSEMBL cdna:known chromosome:CEL140:V:2461148:2464194:-1 gene:F56A4.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2461147-2464194(-) 92.42 100.0
chrV:2353186-2356233(-) 92.57 97.42
Public Domain and Genome References
Gene Title

Ensembl Y19D10A.8
F56A4.10
Entrez Gene 186353 Entrez gene
189486 Entrez gene
SwissProt O44595 EMBL-EBI
Q7Z2C3 EMBL-EBI
WormBase CE17005 Wormbase
CE32238 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503651 Y19D10A.8 [Caenorhabditis elegans] gb|AAK68421.2| Hypothetical protein Y19D10A.8 [Caenorhabditis elegans] 0.0
blast AAF39848 Hypothetical protein F56A4.10 [Caenorhabditis elegans] ref|NP_503677.1| F56A4.10 [Caenorhabditis elegans] 0.0
blast CAE58039 Hypothetical protein CBG01115 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503651.2 10 41-62,72-94,101-120,130-152,159-181,222-244,292-314,318-340,352-374,384-406
NP_503677.1 11 41-62,72-94,101-120,130-152,159-181,222-244,292-314,318-340,347-366,381-403,416-435
Y19D10A.8 10 41-62,72-94,101-120,130-152,159-181,222-244,292-314,318-340,352-374,384-406
F56A4.10 11 41-62,72-94,101-120,130-152,159-181,222-244,292-314,318-340,347-366,381-403,416-435
SNAP00000029966 10 13-34,44-66,73-92,102-124,131-153,194-216,264-286,290-309,321-343,358-377
GENEFINDER00000029980 11 36-58,89-110,120-142,149-168,178-200,207-229,270-292,340-362,366-385,397-419,434-453
SNAP00000032947 10 13-34,44-66,73-92,102-124,131-153,194-216,264-286,290-309,321-343,358-377
GENEFINDER00000032951 11 36-58,89-110,120-142,149-168,178-200,207-229,270-292,340-362,366-385,397-419,434-453
Sequence
Target Sequence 
>C. ELEGANS:185708_S_AT
gaaggagaccggttttctcacagcacttccgttcatcctatcagcgatcgccaagttttc
agctggccggatctctgacaagcttactggattgtctgaaagagcaagattcgtattttt
tgcatcgatttttcaaattggtctagtcgccggattggtggtcatggcttttactaccaa
ccgggtgattgctcaaattgcctttaattttgcaattgtatcatctgggttgaatattat
gggagttgtaaagtttgccgatttgccaaaaaatttaatttttgtcaatttgccgatttg
ccagttcgccggaagttttagagagattctttataagattgaaacacttaaagttcgctg
tcttcaacacgtccattacactctgaccgttatgtcgttcactgcctacgcctcacaatt
cctgtccccaactttagtcagcataatctgcccgaattatactaacgaacagtggaaaac
atttttcctggttatcagcggactcattgttgcctgcaacgcaggattcccatttttggc
aaggtccgatgcggcgggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGAGACCGGTTTTCTCACAGCA 522 333 777 Antisense
GATCGCCAAGTTTTCAGCTGGCCGG 537 415 822 Antisense
CCGGATCTCTGACAAGCTTACTGGA 590 263 843 Antisense
CTAGTCGCCGGATTGGTGGTCATGG 665 223 919 Antisense
GGTCATGGCTTTTACTACCAACCGG 400 491 936 Antisense
TAAAGTTCGCTGTCTTCAACACGTC 548 637 1125 Antisense
TCAACACGTCCATTACACTCTGACC 432 629 1140 Antisense
CTCTGACCGTTATGTCGTTCACTGC 182 231 1157 Antisense
TATCAGCGGACTCATTGTTGCCTGC 641 659 1269 Antisense
GCAACGCAGGATTCCCATTTTTGGC 52 319 1292 Antisense
TTGGCAAGGTCCGATGCGGCGGGAT 247 709 1312 Antisense
Sequence Source Affymetrix Proprietary Database