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[185698_at]

Description Y62E10A.5 /REP_DB=WormBase Gene ID /WP=CE22693 /TR=Q9U1Y0 /GB=CAB60594.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 62      4grp_high_8_8 > 18      6grp_highest_nonorm > 82      6grp_total > 77      4grp_total > 29      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 227
1 2 3 4 range
A 460.2
(3)
P 		319.2
(-1.4)
P 		269.3
(-3)
P 		419.2
(1.7)
P 		191
B 345.3
(-0.2)
P 		347.8
(-0)
P 		295
(-3)
P 		401
(3)
P 		106
C 496.5
(3)
P 		419.3
(0.4)
P 		321.2
(-3)
P 		380.1
(-1)
P 		175
avr 434
(3) 		362.1
(-0.1) 		295.2
(-3) 		400.1
(1.5) 		139

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 332
1 2 3 4 5 6 range
A 272.8
(-3)
P 		359.5
(-1.2)
P 		302
(-2.4)
P 		314.4
(-2.1)
P 		326
(-1.9)
P 		557
(3)
P 		284
B 327.4
(-0.4)
P 		225.1
(-3)
P 		316.8
(-0.7)
P 		291.6
(-1.3)
P 		354.7
(0.3)
P 		459.4
(3)
P 		234
C 328
(-3)
P 		333.2
(-2.8)
P 		379.4
(-1.3)
P 		360.7
(-1.9)
P 		358.9
(-2)
P 		510.4
(3)
P 		182
avr 309.4
(-2.9) 		305.9
(-3) 		332.7
(-2.2) 		322.2
(-2.5) 		346.5
(-1.8) 		508.9
(3) 		203
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 167.5
(3)
P 		69.3
(-2.6)
P 		70.4
(-2.5)
P 		61.4
(-3)
P 		106
B 110.1
(3)
P 		85.9
(-0.5)
P 		68.4
(-3)
P 		85.4
(-0.6)
P 		42
C 164.4
(3)
P 		120.5
(0.4)
P 		111.9
(-0.1)
P 		64.1
(-3)
P 		100
avr 147.3
(3) 		91.9
(-1.3) 		83.6
(-2) 		70.3
(-3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 50.3
(-3)
P 		127.9
(2.5)
P 		65.8
(-1.9)
P 		79.7
(-0.9)
P 		98.4
(0.4)
P 		135.6
(3)
P 		85
B 113.9
(-0.6)
P 		74.9
(-3)
P 		76.9
(-2.9)
P 		77.2
(-2.9)
P 		144.1
(1.3)
P 		171.3
(3)
P 		96
C 121.6
(-0)
P 		101.7
(-1.2)
P 		96.6
(-1.5)
P 		72.5
(-3)
P 		116.1
(-0.3)
P 		170.9
(3)
P 		98
avr 95.3
(-1.6) 		101.5
(-1.2) 		79.8
(-2.8) 		76.5
(-3) 		119.5
(0.1) 		159.3
(3) 		83
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185698_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9278
Sequence Type Exemplar sequence
Representative Public ID Y62E10A.5 NCBI
Target Description Y62E10A.5 /REP_DB=WormBase Gene ID /WP=CE22693 /TR=Q9U1Y0 /GB=CAB60594.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070169(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070169 NCBI Caenorhabditis elegans AlkylGuanine DNA alkylTransferase family member (agt-1) (agt-1) mRNA, complete cds. 11/11 None
SNAP00000008071 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13368493:13369381:-1 11/11 None
GENEFINDER00000008096 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13368493:13369414:-1 11/11 None
Y62E10A.5 ENSEMBL cdna:known chromosome:CEL140:IV:13368428:13369414:-1 gene:Y62E10A.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13368498-13369420(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol agt-1
Ensembl Y62E10A.5
Entrez Gene 190454 Entrez gene
SwissProt Q9U1Y0 EMBL-EBI
WormBase CE38250 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6307 DNA dealkylation inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16765 transferase activity, transferring alkyl or aryl (other than methyl) groups inferred from direct assay QuickGO AmiGO
3684 damaged DNA binding inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60594 Hypothetical protein Y62E10A.5 [Caenorhabditis elegans] ref|NP_502570.2| AlkylGuanine DNA alkylTransferase family member (agt-1) [Caenorhabditis elegans] 1.0E-80
blast CAB60594 Hypothetical protein Y62E10A.5 [Caenorhabditis elegans] ref|NP_502570.2| AlkylGuanine DNA alkylTransferase family member (agt-1) [Caenorhabditis elegans] 2.0E-80
blast CAE58617 Hypothetical protein CBG01784 [Caenorhabditis briggsae] 2.0E-32
blast AAS60352 bifunctional regulatory protein/DNA repair protein [Yersinia pestis biovar Medievalis str. 91001] ref|NP_667414.1| O6-methylguanine-DNA methyltransferase; transcription activator/repressor [Yersinia pestis KIM] emb|CAC88938.1| bifunctional regulatory protein/DNA repair protein [Yersinia pestis CO92] gb|AAM83665.1| O6-methylguanine-DNA methyltransferase; transcription activator/repressor [Yersinia pestis KIM] ref|NP_991475.1| bifunctional regulatory protein/DNA repair protein [Yersinia pestis biovar Medievalis str. 91001] ref|NP_403736.1| bifunctional regulatory protein/DNA repair protein [Yersinia pestis CO92] pir||AH0009 methylated-DNA-[protein]-cysteine S-methyltransferase (EC 2.1.1.63) [imported] - Yersinia pestis (strain CO92) 4.0E-18
Protein Domains
Database ID Description E-Value
scop a.4.2.Methylated DNA-protein cysteine methyltransferase, C-terminal domain All alpha proteins; DNA/RNA-binding 3-helical bundle; Methylated DNA-protein cysteine methyltransferase, C-terminal domain; Methylated DNA-protein cysteine methyltransferase, C-terminal domain 2.00000007187826E-36
scop a.4.2.Methylated DNA-protein cysteine methyltransferase, C-terminal domain All alpha proteins; DNA/RNA-binding 3-helical bundle; Methylated DNA-protein cysteine methyltransferase, C-terminal domain; Methylated DNA-protein cysteine methyltransferase, C-terminal domain 6.9999999825246E-36
Pfam IPR001497 EMBL-EBI Methylated-DNA-[protein]-cysteine S-methyltransferase 1.3E-40
Pfam IPR001497 EMBL-EBI Methylated-DNA-[protein]-cysteine S-methyltransferase 1.3E-40
Sequence
Target Sequence 
>C. ELEGANS:185698_AT
atgatcatccgtgagtgcgtctatgaagtcgtagagacggatcagggacaggctctgatt
gctgaatgtgttggaagtcagaaagttgtggcgatttatcttcttgatgatgatctagac
gatcagatggaggaacttcatgacgcctatcccacagttaacttcattccaggaaaacca
acacaaagctccggtattgtgcaagctctgaatacacttgattcaacgatcaaagtcaat
gcggtacacaaaggagacacggctttcggaatgcaagtttacagcgccattcaaaaaatc
ccaaaaggagaaactcgctcatattcagatattgctcgagaaatcgggaatccgtcggcg
gtgagagctgttgcctcggcatgtgctcgcaacaacttggcctacattgttccgtgtcat
cgtgtgatcggcagcactggaaatctatcaggatatcgttggggaatcgcgaagaagcgt
cgcctattgcaggcagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGATCATCCGTGAGTGCGTCTATG 97 47 46 Antisense
GGGACAGGCTCTGATTGCTGAATGT 353 495 90 Antisense
TGGAGGAACTTCATGACGCCTATCC 445 549 173 Antisense
GCCTATCCCACAGTTAACTTCATTC 615 281 190 Antisense
GCTCCGGTATTGTGCAAGCTCTGAA 485 297 233 Antisense
GAGACACGGCTTTCGGAATGCAAGT 236 393 299 Antisense
GAATCCGTCGGCGGTGAGAGCTGTT 373 329 393 Antisense
GGCATGTGCTCGCAACAACTTGGCC 41 537 423 Antisense
ACAACTTGGCCTACATTGTTCCGTG 598 111 437 Antisense
GTGTCATCGTGTGATCGGCAGCACT 554 485 459 Antisense
GAAGCGTCGCCTATTGCAGGCAGAA 42 343 519 Antisense
Sequence Source Affymetrix Proprietary Database