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[185693_at]

Description W02F12.2 /REP_DB=WormBase Gene ID /WP=CE14474 /TR=O45145 /GB=AAC04458.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 33      4grp_highest_8_8 > 14      4grp_high_8_8 > 23      6grp_highest_nonorm > 51      6grp_total > 21      4grp_total > 16      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 623
1 2 3 4 range
A 385.1
(-3)
P 		500.7
(-1.4)
P 		614.7
(0.2)
P 		819.9
(3)
P 		435
B 327.1
(-3)
P 		458.7
(-1.7)
P 		445.7
(-1.9)
P 		949.8
(3)
P 		623
C 417.2
(-3)
P 		503.6
(-1.6)
P 		630.2
(0.5)
P 		783.8
(3)
P 		367
avr 376.5
(-3) 		487.7
(-1.6) 		563.5
(-0.6) 		851.2
(3) 		475

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 501
1 2 3 4 5 6 range
A 591.3
(0.2)
P 		773.6
(2.5)
P 		817.3
(3)
P 		570.8
(-0.1)
P 		497.2
(-1)
P 		337.3
(-3)
P 		480
B 643.5
(0.8)
P 		807.9
(3)
P 		560.8
(-0.4)
P 		612.2
(0.3)
P 		479.1
(-1.5)
P 		366.1
(-3)
P 		442
C 526
(-0.4)
P 		802.3
(3)
P 		636.2
(0.9)
P 		622.4
(0.8)
P 		511.6
(-0.6)
P 		316.6
(-3)
P 		486
avr 586.9
(0.3) 		794.6
(3) 		671.4
(1.4) 		601.8
(0.5) 		496
(-0.9) 		340
(-3) 		455
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 140.2
(0.6)
P 		108.7
(-3)
P 		160.7
(3)
P 		120
(-1.7)
P 		52
B 104.3
(-2.9)
P 		113.3
(-2.4)
P 		103.3
(-3)
P 		202.4
(3)
P 		99
C 138.1
(-2.6)
P 		144.8
(-2.1)
P 		219.6
(3)
P 		132.2
(-3)
P 		87
avr 127.5
(-2.2) 		122.3
(-3) 		161.2
(3) 		151.5
(1.5) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 193
1 2 3 4 5 6 range
A 109
(-2.2)
P 		275.1
(3)
P 		178
(-0)
P 		144.8
(-1.1)
P 		150
(-0.9)
P 		82.1
(-3)
P 		193
B 223.9
(1)
P 		268.9
(3)
P 		136.2
(-3)
P 		162.2
(-1.8)
P 		194.7
(-0.4)
P 		136.5
(-3)
P 		133
C 195
(0.8)
P 		244.8
(3)
P 		161.9
(-0.6)
P 		125.2
(-2.2)
P 		165.6
(-0.4)
P 		106
(-3)
P 		139
avr 176
(-0.4) 		262.9
(3) 		158.7
(-1) 		144.1
(-1.6) 		170.1
(-0.6) 		108.2
(-3) 		155
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185693_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12149
Sequence Type Exemplar sequence
Representative Public ID W02F12.2 NCBI
Target Description W02F12.2 /REP_DB=WormBase Gene ID /WP=CE14474 /TR=O45145 /GB=AAC04458.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072296(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072296 NCBI Caenorhabditis elegans W02F12.2 (W02F12.2) mRNA, complete cds. 11/11 None
SNAP00000016552 ENSEMBL cdna:SNAP chromosome:CEL140:V:6697650:6700245:-1 11/11 None
GENEFINDER00000016562 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6697650:6700245:-1 11/11 None
W02F12.2 ENSEMBL cdna:known chromosome:CEL140:V:6697810:6700246:-1 gene:W02F12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6697650-6700246(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W02F12.2
Entrez Gene 189124 Entrez gene
SwissProt O45145 EMBL-EBI
WormBase CE33333 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.3339.1.S1_AT similar to alkaline ceramidase 2 cfa
CANINE_2:CFAAFFX.3340.1.S1_S_AT similar to alkaline ceramidase 2 cfa
DROSOPHILA_2:1625491_AT brain washing dm
DROSGENOME1:146530_AT brain washing dm
CHICKEN:GGAAFFX.9581.1.S1_AT similar to cancer related gene-liver 1 gga
HU35KSUBC:RC_H90280_AT N-acylsphingosine amidohydrolase 3-like hs
HG-U95B:45979_AT N-acylsphingosine amidohydrolase 3-like hs
MOUSE430_2:1421496_AT RIKEN cDNA 2410116I05 gene mm
MOE430A:1421496_AT RIKEN cDNA 2410116I05 gene mm
MOUSE430A_2:1421496_AT RIKEN cDNA 2410116I05 gene mm
MG-U74BV2:163674_AT RIKEN cDNA 2410116I05 gene mm
MOE430A:1451355_AT RIKEN cDNA 2410116I05 gene mm
MOUSE430A_2:1451355_AT RIKEN cDNA 2410116I05 gene mm
MOUSE430_2:1451355_AT RIKEN cDNA 2410116I05 gene mm
MU19KSUBB:TC31696_AT RIKEN cDNA 2410116I05 gene (2410116I05Rik), mRNA mm
MU19KSUBC:TC35337_AT RIKEN cDNA 2410116I05 gene (2410116I05Rik), mRNA mm
RAE230B:1391791_AT N-acylsphingosine amidohydrolase 3-like (predicted) rn
RAT230_2:1391791_AT N-acylsphingosine amidohydrolase 3-like (predicted) rn
RAE230B:1380923_AT N-acylsphingosine amidohydrolase 3-like (predicted) rn
RAT230_2:1380923_AT N-acylsphingosine amidohydrolase 3-like (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6672 ceramide metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
139 Golgi membrane inferred from electronic annotation QuickGO AmiGO
5789 endoplasmic reticulum membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAE71933 Hypothetical protein CBG18997 [Caenorhabditis briggsae] 1.0E-150
blast NP_504697 W02F12.2 [Caenorhabditis elegans] gb|AAC04458.2| Hypothetical protein W02F12.2 [Caenorhabditis elegans] 1.0E-148
blast CAE71933 Hypothetical protein CBG18997 [Caenorhabditis briggsae] 1.0E-142
blast XP_623814 PREDICTED: similar to GA12666-PA [Apis mellifera] 3.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR008901 EMBL-EBI Alkaline phytoceramidase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504697.2 7 32-54,61-83,98-117,124-143,148-166,179-196,211-233
W02F12.2 7 32-54,61-83,98-117,124-143,148-166,179-196,211-233
SNAP00000016552 7 32-54,61-83,98-117,124-143,148-166,179-196,211-233
GENEFINDER00000016562 7 32-54,61-83,98-117,124-143,148-166,179-196,211-233
Sequence
Target Sequence 
>C. ELEGANS:185693_AT
aactctcgtccgatgggttgttctaattgtaacagctctcgtatctggtctctgcttcct
tgaaccaaatctcaatgcaatcgctttgatgctgttttccattccagctgctgtagtgat
aaactatgaaggaaaacagtctggaatcccagacatcgagtcatttccaagtcgaattct
tgcactttggggagttgcattttccttctggtttgctgatcgtcttctctgcgatttttg
gctttatttgggaaccccatacctccatgctctgttccatttgctcgccggacttgccgg
ctacacaattttcatcatgttctcgatgattgatattgagagcagaacgaaaactcacaa
atacacagccgcagtccgttattttccgggcaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTCTCGTCCGATGGGTTGTTCTA 462 137 384 Antisense
TGTAACAGCTCTCGTATCTGGTCTC 574 559 411 Antisense
TCTCTGCTTCCTTGAACCAAATCTC 136 613 432 Antisense
TCGCTTTGATGCTGTTTTCCATTCC 9 599 464 Antisense
GAATCCCAGACATCGAGTCATTTCC 688 329 527 Antisense
GTCGAATTCTTGCACTTTGGGGAGT 665 471 554 Antisense
GCATTTTCCTTCTGGTTTGCTGATC 419 307 580 Antisense
GTCTTCTCTGCGATTTTTGGCTTTA 91 469 605 Antisense
TTATTTGGGAACCCCATACCTCCAT 138 667 627 Antisense
TGCCGGCTACACAATTTTCATCATG 604 585 678 Antisense
CGCAGTCCGTTATTTTCCGGGCAAG 455 253 753 Antisense
Sequence Source Affymetrix Proprietary Database