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[185679_at]

Description Y47D3A.15 /REP_DB=WormBase Gene ID /WP=CE22044 /TR=Q9NAH7 /GB=CAB55074.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 13      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 89.7
(-1.2)
A 		94.3
(-0.9)
A 		54.3
(-3)
A 		170.8
(3)
A 		117
B 141.6
(1.2)
A 		19.1
(-3)
A 		67.5
(-1.3)
A 		193.2
(3)
A 		174
C 86.9
(-0.6)
A 		56.8
(-2)
A 		33.9
(-3)
A 		168.2
(3)
A 		134
avr 106.1
(-0.4) 		56.7
(-2.8) 		51.9
(-3) 		177.4
(3) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 9.4
(-1.4)
A 		9.8
(-1.1)
A 		16.4
(3)
A 		8.3
(-2.1)
A 		6.8
(-3)
A 		8
(-2.3)
A 		10
B 10
(-3)
A 		13.9
(-2.8)
A 		21.9
(-2.4)
A 		16
(-2.7)
A 		15.6
(-2.7)
A 		132.7
(3)
A 		123
C 17.1
(-2.4)
A 		7.2
(-3)
A 		11
(-2.8)
A 		9
(-2.9)
A 		12.9
(-2.6)
A 		99.3
(3)
A 		92
avr 12.2
(-2.8) 		10.3
(-3) 		16.4
(-2.5) 		11.1
(-2.9) 		11.8
(-2.9) 		80
(3) 		70
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 32.7
(3)
A 		20.5
(-1)
A 		14.2
(-3)
A 		25
(0.5)
A 		19
B 45.2
(3)
A 		4.7
(-3)
A 		15.6
(-1.4)
A 		41.2
(2.4)
A 		41
C 28.8
(3)
A 		16.3
(-1.4)
A 		11.8
(-3)
A 		28.4
(2.9)
A 		17
avr 35.6
(3) 		13.8
(-3) 		13.9
(-3) 		31.5
(1.9) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 1.7
(-3)
A 		3.5
(2.7)
A 		3.6
(3)
A 		2.1
(-1.7)
A 		2
(-2.1)
A 		1.9
(-2.4)
A 		2
B 3.5
(-3)
A 		4.6
(-2.9)
A 		5.3
(-2.8)
A 		4.3
(-2.9)
A 		6.3
(-2.6)
A 		49.5
(3)
A 		46
C 6.3
(-2.1)
A 		2.2
(-2.9)
A 		2.8
(-2.8)
A 		1.8
(-3)
A 		4.2
(-2.5)
A 		33.3
(3)
A 		32
avr 3.8
(-2.7) 		3.4
(-2.8) 		3.9
(-2.7) 		2.7
(-3) 		4.2
(-2.6) 		28.2
(3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185679_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6984
Sequence Type Exemplar sequence
Representative Public ID Y47D3A.15 NCBI
Target Description Y47D3A.15 /REP_DB=WormBase Gene ID /WP=CE22044 /TR=Q9NAH7 /GB=CAB55074.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067045(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067045 NCBI Caenorhabditis elegans Y47D3A.15 (Y47D3A.15) mRNA, complete cds. 11/11 None
SNAP00000007213 ENSEMBL cdna:SNAP chromosome:CEL140:III:11237028:11260141:1 11/11 None
GENEFINDER00000007273 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11237028:11237964:1 11/11 None
Y47D3A.15 ENSEMBL cdna:known chromosome:CEL140:III:11237028:11237964:1 gene:Y47D3A.15 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11237023-11237960(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y47D3A.15
Entrez Gene 176552 Entrez gene
SwissProt Q9NAH7 EMBL-EBI
WormBase CE22044 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246028_AT 5'-AMP-activated protein kinase beta-2 subunit, putative at
CANINE_2:CFAAFFX.16968.1.S1_AT similar to 5-AMP-activated protein kinase, beta-2 subunit (AMPK beta-2 chain) cfa
CHICKEN:GGAAFFX.1098.1.S1_S_AT similar to 5-AMP-activated protein kinase, beta-2 subunit (AMPK beta-2 chain) gga
CHICKEN:GGAAFFX.24813.1.S1_AT similar to 5-AMP-activated protein kinase, beta-2 subunit (AMPK beta-2 chain) gga
HG-U95AV2:36705_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HU35KSUBA:RC_AA400251_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HU35KSUBA:W90186_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HU35KSUBC:RC_N78582_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
U133_X3P:HS2.399853.1.S1_3P_S_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
U133_X3P:HS.50732.0.S1_3P_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
U133_X3P:HS.35304.0.S1_3P_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HG-U133_PLUS_2:1558027_S_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HG-U133A_2:214474_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HG-FOCUS:214474_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HG-U133_PLUS_2:214474_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HG-U133A:214474_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HG-U133_PLUS_2:225278_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HG-U133B:225278_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HG-U95B:51254_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
HG-U95D:78707_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit hs
RAE230A:1369271_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit rn
RAT230_2:1369271_AT protein kinase, AMP-activated, beta 2 non-catalytic subunit rn
RAE230B:1378845_AT Protein kinase, AMP-activated, beta 2 non-catalytic subunit rn
RAT230_2:1378845_AT Protein kinase, AMP-activated, beta 2 non-catalytic subunit rn
YEAST_2:1776136_AT One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Sc
Protein Similarities
Method ID Description E-Value
blast CAB55075 Hypothetical protein Y47D3A.16 [Caenorhabditis elegans] ref|NP_499447.1| RSK-pSeventy (RSK-p70 kinase) homolog family member (rsks-1) [Caenorhabditis elegans] 0.0
blast CAE67653 Hypothetical protein CBG13216 [Caenorhabditis briggsae] 0.0
blast CAB55074 Hypothetical protein Y47D3A.15 [Caenorhabditis elegans] ref|NP_499446.1| Y47D3A.15 [Caenorhabditis elegans] 1.0E-161
blast CAB55074 Hypothetical protein Y47D3A.15 [Caenorhabditis elegans] ref|NP_499446.1| Y47D3A.15 [Caenorhabditis elegans] 1.0E-160
blast CAE67654 Hypothetical protein CBG13217 [Caenorhabditis briggsae] 1.0E-151
blast CAB04480 Hypothetical protein F55F3.1 [Caenorhabditis elegans] ref|NP_510298.1| F55F3.1 [Caenorhabditis elegans] 6.0E-82
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-60
hanks 1.6.1 AGC group; AGC VI phosphorylate ribosomal protein S6; S6K 1.0E-113
Protein Domains
Database ID Description E-Value
Pfam IPR006828 EMBL-EBI 5-AMP-activated protein kinase, beta subunit, complex-interacting region 5.7E-54
Pfam IPR000719 EMBL-EBI Protein kinase 9.3E-72
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 3.4E-21
Sequence
Target Sequence 
>C. ELEGANS:185679_AT
ggataactggcagacgcggatcccgatggtaaagtcgacaaacgacttttcgacaatcat
cgacctgcaacccggacaatacgagtacaaattccaagtggacggctcgtgggtcgtcga
cgataatcagggaaaggcacaggacgttcatggaaatgagaacaatatgatcaatattca
agactcggattttgcagttttcgaagcgctggatgaagacttccagtcatctacagccgg
ggaggtgctccgtggcgagtcggagagcaccaagaatcatgatactccgaatgacagaga
gctcgaaaaactgcgctccttcacccaagagatcccatcaatggatatgcttcggaaggc
agccggaccaccagtaattccaccacaactcatgcaggtgcttctcaacaaggagacgcc
cgagtcatgcgatccgaatgtgctgccggagccgaatcatgtgatgctcaaccacatgta
cgcgctctccatcaaggacagcgttatggtgctctcatcgacgcaacgctatcgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATAACTGGCAGACGCGGATCCCG 548 507 264 Antisense
TTCGACAATCATCGACCTGCAACCC 347 695 312 Antisense
ATTCCAAGTGGACGGCTCGTGGGTC 692 5 354 Antisense
GAAGACTTCCAGTCATCTACAGCCG 287 339 478 Antisense
TATGCTTCGGAAGGCAGCCGGACCA 464 655 609 Antisense
ACCACAACTCATGCAGGTGCTTCTC 423 85 645 Antisense
CAAGGAGACGCCCGAGTCATGCGAT 620 183 672 Antisense
GAATGTGCTGCCGGAGCCGAATCAT 483 331 699 Antisense
ACGCGCTCTCCATCAAGGACAGCGT 540 91 743 Antisense
AGGACAGCGTTATGGTGCTCTCATC 468 55 758 Antisense
TCTCATCGACGCAACGCTATCGCAA 269 613 776 Antisense
Sequence Source Affymetrix Proprietary Database