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[185675_at]

Description Y53F4B.11 /REP_DB=WormBase Gene ID /WP=CE22400 /TR=Q9NAC0 /GB=CAB61081.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 95      4grp_total > 43      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 5.9
(-3)
A 		74.1
(3)
A 		51.4
(1)
A 		55.6
(1.4)
A 		68
B 61.4
(2.1)
A 		70.7
(3)
A 		9.3
(-3)
A 		8.9
(-3)
A 		62
C 53.5
(1.5)
A 		51.6
(0.9)
A 		38.6
(-3)
A 		58.5
(3)
A 		20
avr 40.3
(-1.7) 		65.5
(3) 		33.1
(-3) 		41
(-1.5) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 92.5
(0.1)
A 		55.3
(-1.5)
A 		21.3
(-3)
A 		89.7
(-0)
P 		158.9
(3)
P 		127.1
(1.6)
P 		138
B 50.6
(-2.4)
A 		40.7
(-3)
A 		72.3
(-1)
A 		68.2
(-1.3)
A 		93.4
(0.3)
P 		135.2
(3)
P 		95
C 41.4
(-1.7)
A 		13.8
(-2.7)
A 		5
(-3)
A 		74.2
(-0.5)
P 		145.9
(2.2)
P 		168.8
(3)
P 		164
avr 61.5
(-1.5) 		36.6
(-2.8) 		32.9
(-3) 		77.4
(-0.6) 		132.7
(2.4) 		143.7
(3) 		111
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 2.1
(-3)
A 		16.1
(3)
A 		13.4
(1.8)
A 		8.1
(-0.4)
A 		14
B 19.6
(3)
A 		17.5
(2.3)
A 		2.2
(-2.9)
A 		1.9
(-3)
A 		18
C 17.7
(3)
A 		14.8
(0.8)
A 		13.4
(-0.3)
A 		9.9
(-3)
A 		8
avr 13.1
(1.1) 		16.1
(3) 		9.7
(-1) 		6.6
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 17.1
(-1.3)
A 		19.7
(-0.9)
A 		4.6
(-3)
A 		22.7
(-0.5)
P 		48
(3)
P 		31
(0.6)
P 		43
B 17.6
(-2.3)
A 		13.6
(-3)
A 		17.6
(-2.3)
A 		18.1
(-2.3)
A 		38
(1)
P 		50.4
(3)
P 		37
C 15.4
(-1.5)
A 		4.2
(-2.7)
A 		1.3
(-3)
A 		14.9
(-1.5)
P 		47.2
(2)
P 		56.5
(3)
P 		55
avr 16.7
(-1.6) 		12.5
(-2.3) 		7.8
(-3) 		18.6
(-1.3) 		44.4
(2.7) 		46
(3) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185675_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2876
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.11 NCBI
Target Description Y53F4B.11 /REP_DB=WormBase Gene ID /WP=CE22400 /TR=Q9NAC0 /GB=CAB61081.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064695(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064695 NCBI Caenorhabditis elegans Y53F4B.11 (Y53F4B.11) mRNA, complete cds. 11/11 None
SNAP00000043046 ENSEMBL cdna:SNAP chromosome:CEL140:II:15010682:15013249:1 11/11 None
GENEFINDER00000043129 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15010682:15013249:1 11/11 None
Y53F4B.11 ENSEMBL cdna:known chromosome:CEL140:II:15010682:15013249:1 gene:Y53F4B.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15010678-15013246(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.11
Entrez Gene 190213 Entrez gene
SwissProt Q9NAC0 EMBL-EBI
WormBase CE22400 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB61081 Hypothetical protein Y53F4B.11 [Caenorhabditis elegans] ref|NP_497096.1| Y53F4B.11 [Caenorhabditis elegans] 1.0E-125
blast CAE73539 Hypothetical protein CBG21003 [Caenorhabditis briggsae] 3.0E-97
Sequence
Target Sequence 
>C. ELEGANS:185675_AT
gaaatcgctaagcgagctgctccgccgccgggtgtcccggatgccgccgattttccgctt
cacgacattgtgaaggagttggggctcgataagccggtcgatgggacggatcccgagttt
tatgaggagctgaaggccaaggacgcgaatacggtctacaagaacatgaaagatgttccg
gattccatcgccgccaagtaccttcccgacctggctcgacgcttcgtggaattcgaacga
cgtgtgaaacgtgtcgaacggatgctttgggccctcccgcgtgaggatcgtagcctcgaa
gaagatcgattcgaaattctcaccgaacttctcgacaagagttgccaaggattggagatt
tgggaggagcactgtgagcgaaaaatcccacttgggcatcgttgtgtgctcgagggagag
ctcattcatctgatcacttcgaaattcgatttaatcgataaaatttgcggagaatttgat
aagttgaagaataaacgctcggaggtcaacgatgaacgtgatatgctccgctacgaaatc
c

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATCGCTAAGCGAGCTGCTCCGC 196 363 166 Antisense
GATTTTCCGCTTCACGACATTGTGA 515 429 214 Antisense
TAAGCCGGTCGATGGGACGGATCCC 500 613 255 Antisense
GAGGAGCTGAAGGCCAAGGACGCGA 288 403 289 Antisense
TGGCTCGACGCTTCGTGGAATTCGA 379 545 377 Antisense
ACGTGTCGAACGGATGCTTTGGGCC 17 95 414 Antisense
TCCCGCGTGAGGATCGTAGCCTCGA 261 589 440 Antisense
CGAAATTCTCACCGAACTTCTCGAC 245 253 477 Antisense
ATCCCACTTGGGCATCGTTGTGTGC 468 33 550 Antisense
GAGAGCTCATTCATCTGATCACTTC 417 395 581 Antisense
CGTGATATGCTCCGCTACGAAATCC 215 245 682 Antisense
Sequence Source Affymetrix Proprietary Database