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[185673_s_at]

Description F56A4.12 /REP_DB=WormBase Gene ID /WP=CE25010 /TR=Q9N5Q8 /GB=AAF39850.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 36      4grp_total > 12      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 125
(0.2)
P 		117.4
(-0.3)
A 		69.4
(-3)
A 		175
(3)
P 		106
B 116.3
(1.2)
A 		84.7
(-3)
A 		123.8
(2.2)
A 		129.4
(3)
A 		45
C 89.7
(-2.9)
P 		98.6
(-2.1)
A 		88.5
(-3)
A 		156.8
(3)
P 		68
avr 110.3
(-1.4) 		100.2
(-2.4) 		93.9
(-3) 		153.7
(3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 94.4
(-0.2)
A 		140.9
(3)
P 		68.6
(-2)
M 		54.8
(-3)
A 		90.6
(-0.5)
A 		96.7
(-0.1)
A 		86
B 111.1
(1.7)
P 		129.8
(3)
P 		109.1
(1.5)
A 		99.5
(0.8)
P 		45.5
(-3)
A 		114.1
(1.9)
P 		84
C 103.6
(2.3)
A 		109.3
(3)
M 		64.2
(-2.5)
A 		67.1
(-2.1)
A 		59.9
(-3)
A 		84.7
(0)
P 		49
avr 103
(0.7) 		126.7
(3) 		80.6
(-1.5) 		73.8
(-2.2) 		65.3
(-3) 		98.5
(0.2) 		61
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 45.5
(3)
P 		25.5
(-1.4)
A 		18.1
(-3)
A 		25.6
(-1.4)
P 		27
B 37.1
(3)
A 		20.9
(-3)
A 		28.7
(-0.1)
A 		27.6
(-0.5)
A 		16
C 29.7
(1.5)
P 		28.4
(-0.3)
A 		30.8
(3)
A 		26.4
(-3)
P 		4
avr 37.4
(3) 		24.9
(-3) 		25.9
(-2.5) 		26.5
(-2.2) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 17.4
(-2.4)
A 		50.1
(3)
P 		14.9
(-2.8)
M 		13.9
(-3)
A 		27.3
(-0.8)
A 		23.6
(-1.4)
A 		36
B 38.6
(1.9)
P 		43.2
(3)
P 		26.5
(-1.1)
A 		26.3
(-1.1)
P 		18.5
(-3)
A 		42.5
(2.8)
P 		25
C 38.4
(3)
A 		33.3
(1.8)
M 		16.3
(-2.3)
A 		13.5
(-3)
A 		19.4
(-1.6)
A 		28.3
(0.6)
P 		25
avr 31.5
(0.4) 		42.2
(3) 		19.2
(-2.7) 		17.9
(-3) 		21.7
(-2.1) 		31.5
(0.4) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185673_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15714
Sequence Type Exemplar sequence
Representative Public ID F56A4.12 NCBI
Target Description F56A4.12 /REP_DB=WormBase Gene ID /WP=CE25010 /TR=Q9N5Q8 /GB=AAF39850.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071253(11), NM_071279(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071253 NCBI Caenorhabditis elegans Y19D10A.11 (Y19D10A.11) mRNA, complete cds. 11/11 None
NM_071279 NCBI Caenorhabditis elegans F56A4.12 (F56A4.12) mRNA, complete cds. 11/11 None
SNAP00000029968 ENSEMBL cdna:SNAP chromosome:CEL140:V:2361555:2372351:-1 9/11 None
GENEFINDER00000029985 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2368772:2373213:-1 11/11 None
SNAP00000032949 ENSEMBL cdna:SNAP chromosome:CEL140:V:2469516:2480312:-1 9/11 None
Y19D10A.11 ENSEMBL cdna:known chromosome:CEL140:V:2368772:2373530:-1 gene:Y19D10A.11 11/11 None
F56A4.12 ENSEMBL cdna:known chromosome:CEL140:V:2476733:2481491:-1 gene:F56A4.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2368771-2373530(-) 96.19 100.0
chrV:2476732-2481491(-) 96.19 100.0
Public Domain and Genome References
Gene Title

Ensembl Y19D10A.11
F56A4.12
Entrez Gene 186355 Entrez gene
189488 Entrez gene
SwissProt Q9N5Q8 EMBL-EBI
Q966D7 EMBL-EBI
WormBase CE25010 Wormbase
CE23774 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503654 Y19D10A.11 [Caenorhabditis elegans] gb|AAK68411.2| Hypothetical protein Y19D10A.11 [Caenorhabditis elegans] 0.0
blast AAF39850 Hypothetical protein F56A4.12 [Caenorhabditis elegans] ref|NP_503680.1| F56A4.12 [Caenorhabditis elegans] 0.0
blast CAE58039 Hypothetical protein CBG01115 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503654.2 11 53-75,106-127,137-159,166-185,195-217,224-246,287-309,355-377,381-403,416-438,448-470
NP_503680.1 11 55-77,90-111,121-143,150-169,179-201,208-230,271-293,339-361,365-387,400-422,432-454
Y19D10A.11 11 53-75,106-127,137-159,166-185,195-217,224-246,287-309,355-377,381-403,416-438,448-470
F56A4.12 11 55-77,90-111,121-143,150-169,179-201,208-230,271-293,339-361,365-387,400-422,432-454
SNAP00000029968 26 68-89,99-121,128-147,157-179,186-208,250-272,285-304,319-341,348-365,370-392,413-435,445-464,471-493,503-525,532-554,631-653,660-682,687-709,716-738,748-767,799-821,836-854,867-889,893-915,928-950,960-982
GENEFINDER00000029985 11 30-52,112-133,143-165,172-191,201-223,264-286,301-319,332-354,358-380,393-415,425-447
SNAP00000032949 26 68-89,99-121,128-147,157-179,186-208,250-272,285-304,319-341,348-365,370-392,413-435,445-464,471-493,503-525,532-554,631-653,660-682,687-709,716-738,748-767,799-821,836-854,867-889,893-915,928-950,960-982
Sequence
Target Sequence 
>C. ELEGANS:185673_S_AT
gttctaatcacttggacgtcctgcttcggaggcaacatggccttcttcgccctttcgctt
tatggtccaacttatctccgagaaattctcaagtttgatgtaagggagactggattcttg
tctgcgcttccatttattctatcagcgatcgtcaagttcggagcaggacagctctcagac
cgaatgacattcctttcggaaaaggcacgcttcgtgtttttcgctgcaacctctcaaatt
ggattcgccgtcgggctagtggttatggcgttcacttcggatcgtctaattgctcaaatc
gcgttcaactttgcaattgtgtcttctggcttgaatataatgggtgtgattaagtgtatt
cagttgcgctgccgtcaacacgttcacttcgcgattgctgtaatctccttcacagcctac
gttgtccaattccttgcgccaataattgtcagcattatttgcccggacaacactccagaa
cagtggacaattctgttcctattcatcactggtgttatcatcgtctgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCTAATCACTTGGACGTCCTGCT 534 443 823 Antisense
TCGGAGGCAACATGGCCTTCTTCGC 402 601 848 Antisense
GACAGCTCTCAGACCGAATGACATT 147 369 989 Antisense
AAAGGCACGCTTCGTGTTTTTCGCT 488 121 1023 Antisense
AAATTGGATTCGCCGTCGGGCTAGT 652 113 1058 Antisense
GCGTTCACTTCGGATCGTCTAATTG 438 291 1090 Antisense
AACTTTGCAATTGTGTCTTCTGGCT 432 139 1129 Antisense
ACGTTCACTTCGCGATTGCTGTAAT 571 89 1202 Antisense
AGCATTATTTGCCCGGACAACACTC 229 79 1273 Antisense
ATTCTGTTCCTATTCATCACTGGTG 232 11 1312 Antisense
ATCACTGGTGTTATCATCGTCTGCA 27 29 1327 Antisense
Sequence Source Affymetrix Proprietary Database