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[185662_at]

Description F20E11.12 /REP_DB=WormBase Gene ID /WP=CE18621 /TR=Q9XV76 /GB=CAB04149.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 03      6grp_cgc > 72      6grp_cgc_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 11.2
(-3)
A 		12.4
(-2.8)
A 		14.7
(-2.6)
A 		58.3
(3)
A 		47
B 14.3
(-3)
A 		21.4
(-1.8)
A 		21.3
(-1.8)
A 		50.4
(3)
P 		36
C 32.9
(-0.9)
P 		11.8
(-3)
A 		12.9
(-2.9)
A 		72.9
(3)
M 		61
avr 19.5
(-2.4) 		15.2
(-3) 		16.3
(-2.9) 		60.5
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 12.5
(-0.2)
A 		25.3
(3)
A 		16.7
(0.9)
A 		1.3
(-2.9)
A 		1.8
(-2.8)
A 		1
(-3)
A 		24
B 15.2
(1.8)
A 		4.4
(-1.8)
A 		18.7
(3)
A 		1.7
(-2.7)
A 		0.8
(-3)
A 		2.4
(-2.5)
A 		18
C 1.6
(-3)
A 		29.3
(3)
A 		24.5
(2)
A 		22.9
(1.6)
A 		2.2
(-2.9)
A 		2.6
(-2.8)
A 		28
avr 9.8
(-0.3) 		19.7
(2.9) 		20
(3) 		8.6
(-0.7) 		1.6
(-3) 		2
(-2.9) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 4.1
(-1.6)
A 		2.7
(-3)
A 		3.9
(-1.8)
A 		8.5
(3)
A 		6
B 4.6
(-3)
A 		5.3
(-2.3)
A 		4.9
(-2.7)
A 		10.7
(3)
P 		6
C 10.9
(2.1)
P 		3.4
(-3)
A 		4.5
(-2.3)
A 		12.3
(3)
M 		9
avr 6.5
(-0.6) 		3.8
(-3) 		4.4
(-2.5) 		10.5
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 2.3
(-1.6)
A 		9
(3)
A 		3.6
(-0.7)
A 		0.3
(-3)
A 		0.6
(-2.8)
A 		0.3
(-3)
A 		9
B 5.3
(3)
A 		1.5
(-1.6)
A 		4.5
(2)
A 		0.5
(-2.8)
A 		0.3
(-3)
A 		0.9
(-2.3)
A 		5
C 0.6
(-3)
A 		8.9
(3)
A 		6.2
(1)
A 		4.6
(-0.1)
A 		0.7
(-2.9)
A 		0.9
(-2.8)
A 		8
avr 2.7
(-0.8) 		6.5
(3) 		4.8
(1.3) 		1.8
(-1.7) 		0.5
(-3) 		0.7
(-2.8) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185662_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12874
Sequence Type Exemplar sequence
Representative Public ID F20E11.12 NCBI
Target Description F20E11.12 /REP_DB=WormBase Gene ID /WP=CE18621 /TR=Q9XV76 /GB=CAB04149.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075011(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075011 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-154) (srh-154) mRNA, complete cds. 11/11 A 
GENEFINDER00000027637 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17441688:17444351:1 11/11 None
F20E11.12 ENSEMBL cdna:known chromosome:CEL140:V:17443133:17444351:1 gene:F20E11.12 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17443132-17444351(+) 100.0 100.0
chrV:17182726-17184134(+) 93.35 93.35
Public Domain and Genome References
Gene Title
Gene Symbol srh-154
Ensembl F20E11.12
Entrez Gene 184740 Entrez gene
SwissProt Q9XV76 EMBL-EBI
WormBase CE18621 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04149 Hypothetical protein F20E11.12 [Caenorhabditis elegans] ref|NP_507412.1| Serpentine Receptor, class H family member (srh-154) [Caenorhabditis elegans] 0.0
blast T21162 hypothetical protein F20E11.11 - Caenorhabditis elegans 0.0
blast CAB63199 Hypothetical protein F40D4.2 [Caenorhabditis elegans] ref|NP_507349.1| Serpentine Receptor, class H family member (srh-155) [Caenorhabditis elegans] 1.0E-174
blast CAB63199 Hypothetical protein F40D4.2 [Caenorhabditis elegans] ref|NP_507349.1| Serpentine Receptor, class H family member (srh-155) [Caenorhabditis elegans] 1.0E-173
blast CAB63200 Hypothetical protein F40D4.3 [Caenorhabditis elegans] ref|NP_507350.1| Serpentine Receptor, class H family member (srh-159) [Caenorhabditis elegans] 1.0E-122
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.9E-52
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.9E-87
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507412.1 6 53-75,95-117,138-160,197-219,240-262,277-296
F20E11.12 6 53-75,95-117,138-160,197-219,240-262,277-296
GENEFINDER00000027637 13 15-37,50-72,87-106,119-141,180-202,215-245,300-322,404-426,446-468,489-511,548-570,591-613,628-647
Sequence
Target Sequence 
>C. ELEGANS:185662_AT
attgtatcggttactgtttgtcacagctaattatgtttatgctacattgtaccctgctcc
catatactttttgcttcccgaccaagaatatggaagaattttatcgaaaagtaaaaatcc
gtgtattccgaacgaatatttaaaccatcctaatttctttttgcttgatctcgatggaaa
gtatacttcaatttgtatcctgcttatgttgagttctctggtctctcaaatgttttggca
aattggactgattttccgtcagatgctcaaaaatccgtccgtttctcaaaatacgcaccg
actacaataccagtttttaattgcaatgagcttgcaaggcaccattccaatgattatcat
tgtttttccagcttttttctatgttgtctcaattatgttaaattatcataatcaaggtgc
aaataacttatcgtttcttatcatttccatgcatggagttctatcaacgttgacaatgct
catggcacacagaccgtatagacaatcgattgtcaaaatgttgaatctgaatttcaataa
ggcaggtggtggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGTATCGGTTACTGTTTGTCACA 280 5 414 Antisense
TTTGCTTCCCGACCAAGAATATGGA 430 667 483 Antisense
AAATCCGTGTATTCCGAACGAATAT 115 119 528 Antisense
GAACGAATATTTAAACCATCCTAAT 224 347 543 Antisense
ACTTCAATTTGTATCCTGCTTATGT 418 103 598 Antisense
ATACCAGTTTTTAATTGCAATGAGC 356 21 720 Antisense
GGTGCAAATAACTTATCGTTTCTTA 483 501 829 Antisense
TTATCGTTTCTTATCATTTCCATGC 82 681 841 Antisense
GTTTCTTATCATTTCCATGCATGGA 246 451 846 Antisense
GCACACAGACCGTATAGACAATCGA 394 317 898 Antisense
GAATTTCAATAAGGCAGGTGGTGGT 246 321 942 Antisense
Sequence Source Affymetrix Proprietary Database