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[185660_s_at]

Description T12A2.14 /REP_DB=WormBase Gene ID /WP=CE18255 /TR=O76834 /GB=AAC24451.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 23      4grp_highest_8_8 > 40      4grp_high_8_8 > 50      6grp_highest_nonorm > 51      6grp_total > 12      4grp_total > 61      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 319
1 2 3 4 range
A 510.2
(2.5)
P 		534.8
(3)
P 		397.1
(0.1)
P 		253.5
(-3)
P 		281
B 545.7
(3)
P 		406.1
(0.4)
P 		444.1
(1.1)
P 		227
(-3)
P 		319
C 511.2
(3)
P 		387.5
(0.2)
P 		442.4
(1.5)
P 		244.6
(-3)
P 		267
avr 522.4
(3) 		442.8
(1.3) 		427.9
(1) 		241.7
(-3) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 390
1 2 3 4 5 6 range
A 563
(0.4)
P 		713.1
(3)
P 		510.2
(-0.5)
P 		580.7
(0.7)
P 		361.4
(-3)
P 		410.1
(-2.2)
P 		352
B 625.3
(3)
P 		624.6
(3)
P 		570.5
(1.5)
P 		533.3
(0.4)
P 		543.9
(0.7)
P 		409.5
(-3)
P 		216
C 475.4
(-0)
P 		631.3
(3)
P 		559.2
(1.6)
P 		474.1
(-0.1)
P 		388.1
(-1.7)
P 		323.1
(-3)
P 		308
avr 554.6
(0.8) 		656.3
(3) 		546.6
(0.6) 		529.4
(0.2) 		431.1
(-1.9) 		380.9
(-3) 		275
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 149
1 2 3 4 range
A 185.8
(3)
P 		116.1
(0.2)
P 		103.8
(-0.3)
P 		37.1
(-3)
P 		149
B 174
(3)
P 		100.3
(-0.5)
P 		102.9
(-0.4)
P 		48.4
(-3)
P 		126
C 169.2
(3)
P 		111.4
(0.3)
P 		154.2
(2.3)
P 		41.2
(-3)
P 		128
avr 176.3
(3) 		109.3
(0) 		120.3
(0.5) 		42.2
(-3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 103.8
(-2.8)
P 		253.6
(3)
P 		111.1
(-2.6)
P 		147.3
(-1.1)
P 		109.1
(-2.6)
P 		99.8
(-3)
P 		154
B 217.6
(2.8)
P 		207.9
(2)
P 		138.5
(-3)
P 		141.3
(-2.8)
P 		221
(3)
P 		152.7
(-2)
P 		83
C 176.2
(2)
P 		192.6
(3)
P 		142.3
(-0.1)
P 		95.3
(-3)
P 		125.6
(-1.1)
P 		108.2
(-2.2)
P 		97
avr 165.9
(-0.2) 		218
(3) 		130.6
(-2.4) 		128
(-2.5) 		151.9
(-1.1) 		120.2
(-3) 		98
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185660_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7311
Sequence Type Exemplar sequence
Representative Public ID T12A2.14 NCBI
Target Description T12A2.14 /REP_DB=WormBase Gene ID /WP=CE18255 /TR=O76834 /GB=AAC24451.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171157(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171157 NCBI Caenorhabditis elegans T12A2.15b (T12A2.15) mRNA, complete cds. 11/11 None
SNAP00000026120 ENSEMBL cdna:SNAP chromosome:CEL140:III:6224557:6229321:-1 11/11 None
GENEFINDER00000026141 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6224557:6236927:-1 11/11 None
T12A2.15b ENSEMBL cdna:known chromosome:CEL140:III:6224430:6226047:-1 gene:T12A2.15 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171156 7/11 Cross Hyb Matching Probes A 
T12A2.15a 7/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175882 2 1 NM_171156 186022_at (11)
NM_171157 185660_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6224555-6225801(-) 100.0 100.0
Public Domain and Genome References
Ensembl T12A2.15
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC24452 Hypothetical protein T12A2.15a [Caenorhabditis elegans] ref|NP_741181.1| T12A2.15a [Caenorhabditis elegans] 0.0
blast CAE70749 Hypothetical protein CBG17495 [Caenorhabditis briggsae] 0.0
blast AAC24444 Hypothetical protein T12A2.5 [Caenorhabditis elegans] ref|NP_498357.1| T12A2.5 [Caenorhabditis elegans] 1.0E-153
blast AAC24451 Hypothetical protein T12A2.15b [Caenorhabditis elegans] ref|NP_741182.1| T12A2.15b [Caenorhabditis elegans] 1.0E-124
blast AAC24451 Hypothetical protein T12A2.15b [Caenorhabditis elegans] ref|NP_741182.1| T12A2.15b [Caenorhabditis elegans] 1.0E-121
blast AAC24452 Hypothetical protein T12A2.15a [Caenorhabditis elegans] ref|NP_741181.1| T12A2.15a [Caenorhabditis elegans] 7.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 2.6E-15
Pfam IPR000008 EMBL-EBI C2 domain 6.6E-30
Pfam IPR000008 EMBL-EBI C2 domain 1.2E-13
Pfam IPR000008 EMBL-EBI C2 domain 2.6E-15
Pfam IPR000008 EMBL-EBI C2 domain 6.6E-30
Pfam IPR000008 EMBL-EBI C2 domain 1.2E-13
Pfam IPR000008 EMBL-EBI C2 domain 2.6E-15
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000026120 2 5-23,30-47
GENEFINDER00000026141 3 12-31,632-654,661-678
Sequence
Target Sequence 
>C. ELEGANS:185660_S_AT
gagatgttgtagcggttgagcctgctaaaactgtcaaccaagacgcggcagatgacgagg
ttgagattaagttgaacgtcgataattatcagatgctccgctccgattctcagggatctt
tgaatagccatggaagatccaacagtcgtcttggtagactatttcgttccaagcacgaga
tgaagaaaagagagactagagctgatgaaaatcgcggagaaatcgaaattcaaattgatt
ttgacgatcttgtgaatcagctcaagatcgctctgattcggtgtcgagacttgatgactt
ttgacaaaaaggatcaatgtaatccttatgtttcggtgaaactggtggcgttggatggaa
ataaggaggttttcaaaaagaaaacaccgacagccaaaaatacgagacatccgcatttcg
ataaccatgtcgaaatcgacataaacccatcagatctactgaatcataaagtagtaatca
atgtgaaagatgacacaaactacggaacatttgttgcaaaaccagttcttggatgtcttg
aaattcgtctggacagcctgatgaatcgtcaactcagccaaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGATGTTGTAGCGGTTGAGCCTGC 657 395 68 Antisense
AAAACTGTCAACCAAGACGCGGCAG 369 129 94 Antisense
GACGCGGCAGATGACGAGGTTGAGA 482 381 109 Antisense
CGATAATTATCAGATGCTCCGCTCC 167 249 147 Antisense
GCTCCGATTCTCAGGGATCTTTGAA 165 299 167 Antisense
GGTAGACTATTTCGTTCCAAGCACG 43 505 220 Antisense
GATCGCTCTGATTCGGTGTCGAGAC 258 417 333 Antisense
ACGAGACATCCGCATTTCGATAACC 267 93 469 Antisense
TCGACATAAACCCATCAGATCTACT 649 599 503 Antisense
GAAATTCGTCTGGACAGCCTGATGA 258 363 607 Antisense
GATGAATCGTCAACTCAGCCAAAGA 632 411 627 Antisense
Sequence Source Affymetrix Proprietary Database