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[185656_s_at]

Description Y73F8A.22 /REP_DB=WormBase Gene ID /WP=CE24656 /TR=Q9NA50 /GB=CAB60550.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 73      4grp_total > 0      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 8.4
(-3)
A 		16.4
(-2.6)
A 		71.4
(0.4)
A 		119.7
(3)
A 		111
B 50.2
(0.9)
A 		7.8
(-3)
A 		24.9
(-1.4)
A 		73.6
(3)
A 		66
C 51.8
(3)
A 		21.8
(-1.2)
A 		9.3
(-3)
A 		40
(1.3)
A 		43
avr 36.8
(-0.9) 		15.3
(-3) 		35.2
(-1.1) 		77.8
(3) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 12.1
(-1.8)
A 		7.8
(-2.6)
A 		37.3
(3)
A 		7.3
(-2.7)
A 		6
(-2.9)
A 		5.7
(-3)
A 		32
B 6.1
(-3)
A 		35
(2.8)
A 		11.5
(-1.9)
A 		12.3
(-1.8)
A 		35.9
(3)
A 		27.5
(1.3)
A 		30
C 5.6
(-3)
A 		22.5
(0.4)
A 		33.9
(2.7)
A 		35.3
(3)
A 		32.3
(2.4)
A 		19.6
(-0.2)
A 		30
avr 7.9
(-3) 		21.8
(1.2) 		27.6
(3) 		18.3
(0.2) 		24.7
(2.1) 		17.6
(-0) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 3
(-3)
A 		3.6
(-2.8)
A 		18.7
(3)
A 		17.5
(2.5)
A 		16
B 16
(3)
A 		1.9
(-3)
A 		5.8
(-1.3)
A 		15.7
(2.9)
A 		14
C 17.1
(3)
A 		6.3
(-1.7)
A 		3.2
(-3)
A 		6.8
(-1.4)
A 		14
avr 12
(2.2) 		3.9
(-3) 		9.2
(0.4) 		13.3
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 2.2
(-2.3)
A 		2.8
(-1.7)
A 		8.1
(3)
A 		1.9
(-2.6)
A 		1.8
(-2.6)
A 		1.4
(-3)
A 		7
B 2.1
(-3)
A 		11.7
(1.6)
A 		2.8
(-2.7)
A 		3.2
(-2.5)
A 		14.6
(3)
A 		10.3
(0.9)
A 		13
C 2.1
(-3)
A 		6.9
(0.4)
A 		8.6
(1.6)
A 		7.1
(0.6)
A 		10.5
(3)
A 		6.6
(0.2)
A 		8
avr 2.1
(-3) 		7.1
(1.3) 		6.5
(0.8) 		4.1
(-1.3) 		9
(3) 		6.1
(0.5) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185656_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10261
Sequence Type Exemplar sequence
Representative Public ID Y73F8A.22 NCBI
Target Description Y73F8A.22 /REP_DB=WormBase Gene ID /WP=CE24656 /TR=Q9NA50 /GB=CAB60550.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070601(11), NM_070456(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070601 NCBI Caenorhabditis elegans Y116A8C.1 (Y116A8C.1) mRNA, complete cds. 11/11 None
NM_070456 NCBI Caenorhabditis elegans Y73F8A.22 (Y73F8A.22) mRNA, complete cds. 11/11 A 
GENEFINDER00000004162 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16900209:16902479:-1 11/11 None
GENEFINDER00000030886 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:15439152:15441370:-1 11/11 None
Y73F8A.22 ENSEMBL cdna:known chromosome:CEL140:IV:15439152:15441370:-1 gene:Y73F8A.22 11/11 A 
Y116A8C.1 ENSEMBL cdna:known chromosome:CEL140:IV:16900209:16902479:-1 gene:Y116A8C.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000004068 4/11 Cross Hyb Matching Probes None
SNAP00000030825 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:15439157-15441376(-) 100.0 100.0
chrIV:16900214-16902485(-) 96.41 96.41
Public Domain and Genome References
Gene Title

Ensembl Y73F8A.22
Y116A8C.1
Entrez Gene 190682 Entrez gene
190991 Entrez gene
SwissProt Q9U2T6 EMBL-EBI
Q9NA50 EMBL-EBI
WormBase CE23313 Wormbase
CE24656 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB55141 Hypothetical protein Y116A8C.1 [Caenorhabditis elegans] ref|NP_503002.1| Y116A8C.1 [Caenorhabditis elegans] 0.0
blast CAB60550 Hypothetical protein Y73F8A.22 [Caenorhabditis elegans] ref|NP_502857.1| Y73F8A.22 [Caenorhabditis elegans] 0.0
blast CAB55141 Hypothetical protein Y116A8C.1 [Caenorhabditis elegans] ref|NP_503002.1| Y116A8C.1 [Caenorhabditis elegans] 1.0E-149
blast CAB60550 Hypothetical protein Y73F8A.22 [Caenorhabditis elegans] ref|NP_502857.1| Y73F8A.22 [Caenorhabditis elegans] 1.0E-148
Sequence
Target Sequence 
>C. ELEGANS:185656_S_AT
gcggtttgcatttcgatccgcacattttccggaccattctgccaaaatcgtgctgcagga
gtagctttgtgtacagaaggaaacgggcttgtcattacaggcgcctatgcgtacgcagaa
ggatacactattggagattatctaacaaagaaaagtaactccgcccttctgcctacacga
tactctaatatgtacttttggatcgacggaatccgctcatcaagtgcccaatacaacatg
tctgacccaactttaaatggtattacatgctttaattggggtccaaactctcaggaggga
gtgctcaatacgtgtgcatttatcgaaggaacatctattgtccggtattgggactgtgat
gccactgaaaacggaaacagttactgcctacgtggcgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGGTTTGCATTTCGATCCGCACAT 466 291 499 Antisense
GACCATTCTGCCAAAATCGTGCTGC 447 383 530 Antisense
GAAACGGGCTTGTCATTACAGGCGC 357 357 578 Antisense
TTACAGGCGCCTATGCGTACGCAGA 705 681 593 Antisense
GGATCGACGGAATCCGCTCATCAAG 693 511 698 Antisense
CGCTCATCAAGTGCCCAATACAACA 303 255 712 Antisense
ATACAACATGTCTGACCCAACTTTA 117 23 729 Antisense
ATTGGGGTCCAAACTCTCAGGAGGG 193 3 773 Antisense
GGAACATCTATTGTCCGGTATTGGG 213 527 826 Antisense
GGTATTGGGACTGTGATGCCACTGA 238 505 842 Antisense
AAACAGTTACTGCCTACGTGGCGCT 60 129 873 Antisense
Sequence Source Affymetrix Proprietary Database