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[185652_at]

Description Y32B12A.4 /REP_DB=WormBase Gene ID /WP=CE19061 /TR=Q9XXH1 /GB=CAA19478.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 53      4grp_total > 38      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 4.1
(-3)
A 		11.6
(3)
A 		10.7
(2.3)
A 		7.3
(-0.4)
A 		8
B 6.3
(3)
A 		3.8
(-0.2)
A 		1.6
(-3)
A 		4.3
(0.4)
A 		5
C 20.1
(3)
A 		3.2
(-2.6)
A 		1.9
(-3)
A 		9.5
(-0.5)
A 		18
avr 10.2
(3) 		6.2
(-1.4) 		4.7
(-3) 		7
(-0.5) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 18.5
(3)
A 		2
(-3)
A 		18.1
(2.9)
A 		2.5
(-2.8)
A 		5.8
(-1.6)
A 		6.4
(-1.4)
A 		17
B 5.6
(-2.5)
A 		4.1
(-2.8)
A 		36
(3)
A 		6.1
(-2.4)
A 		34.3
(2.7)
A 		2.8
(-3)
A 		33
C 5.4
(-1.7)
A 		17.2
(3)
A 		2
(-3)
A 		2.9
(-2.6)
A 		3.1
(-2.6)
A 		8.3
(-0.5)
A 		15
avr 9.8
(-0.6) 		7.8
(-1.4) 		18.7
(3) 		3.8
(-3) 		14.4
(1.3) 		5.8
(-2.2) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 1.5
(-1.6)
A 		2.5
(1.9)
A 		2.8
(3)
A 		1.1
(-3)
A 		2
B 2
(3)
A 		0.9
(-1.1)
A 		0.4
(-3)
A 		0.9
(-1.1)
A 		2
C 6.6
(3)
A 		0.9
(-2.8)
A 		0.7
(-3)
A 		1.6
(-2.1)
A 		6
avr 3.4
(3) 		1.4
(-2.5) 		1.3
(-2.7) 		1.2
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 3.4
(2.1)
A 		0.7
(-2.8)
A 		3.9
(3)
A 		0.6
(-3)
A 		1.7
(-1)
A 		1.6
(-1.2)
A 		3
B 2
(-2.5)
A 		1.4
(-2.8)
A 		8.7
(0.6)
A 		1.6
(-2.7)
A 		13.9
(3)
A 		1
(-3)
A 		13
C 2
(-1.1)
A 		5.3
(3)
A 		0.5
(-3)
A 		0.6
(-2.9)
A 		1
(-2.4)
A 		2.8
(-0.1)
A 		5
avr 2.5
(-0.9) 		2.5
(-0.9) 		4.4
(1.6) 		0.9
(-3) 		5.5
(3) 		1.8
(-1.8) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185652_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12999
Sequence Type Exemplar sequence
Representative Public ID Y32B12A.4 NCBI
Target Description Y32B12A.4 /REP_DB=WormBase Gene ID /WP=CE19061 /TR=Q9XXH1 /GB=CAA19478.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074775(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074775 NCBI Caenorhabditis elegans Y32B12A.4 (Y32B12A.4) mRNA, complete cds. 11/11 None
GENEFINDER00000002062 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16497312:16497599:-1 9/11 None
Y32B12A.4 ENSEMBL cdna:known chromosome:CEL140:V:16497312:16497809:-1 gene:Y32B12A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16497311-16497809(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y32B12A.4
Entrez Gene 189564 Entrez gene
SwissProt Q9XXH1 EMBL-EBI
WormBase CE19061 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA19478 Hypothetical protein Y32B12A.4 [Caenorhabditis elegans] ref|NP_507176.1| Y32B12A.4 [Caenorhabditis elegans] 2.0E-35
blast CAA19478 Hypothetical protein Y32B12A.4 [Caenorhabditis elegans] ref|NP_507176.1| Y32B12A.4 [Caenorhabditis elegans] 2.0E-29
Protein Domains
Database ID Description E-Value
Pfam IPR009564 EMBL-EBI Protein of unknown function DUF1179 3.0E-10
Pfam IPR009564 EMBL-EBI Protein of unknown function DUF1179 3.0E-10
Sequence
Target Sequence 
>C. ELEGANS:185652_AT
atgtttccactcgtgtttctaaaagatttcgcacacctagtcactaaaaattctctggtc
tatccgagtccgcgtaaatcaattagtccaatttttcaaaatccaccggcaaatacttcc
gttgcaagaatcggatcagtggaggaagatactcttgcaaacttaaaatctctcccagca
gagaaatcggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTTCCACTCGTGTTTCTAAAAG 574 47 13 Antisense
AAAAGATTTCGCACACCTAGTCACT 319 129 33 Antisense
CCTAGTCACTAAAAATTCTCTGGTC 269 265 48 Antisense
AAAATTCTCTGGTCTATCCGAGTCC 170 133 59 Antisense
GTCTATCCGAGTCCGCGTAAATCAA 547 469 70 Antisense
GAGTCCGCGTAAATCAATTAGTCCA 669 397 78 Antisense
AATCCACCGGCAAATACTTCCGTTG 40 147 112 Antisense
ACTTCCGTTGCAAGAATCGGATCAG 693 103 127 Antisense
GGAGGAAGATACTCTTGCAAACTTA 584 515 153 Antisense
GCAAACTTAAAATCTCTCCCAGCAG 90 317 169 Antisense
ATCTCTCCCAGCAGAGAAATCGGAT 32 33 180 Antisense
Sequence Source Affymetrix Proprietary Database