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[185646_at]

Description T01B10.2 /REP_DB=WormBase Gene ID /WP=CE12886 /TR=O02153 /GB=AAB52859.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      6grp_highest_nonorm > 35      6grp_total > 73      4grp_total > 57      6grp_highest_sub > 04      6grp_cgc > 75      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8969
1 2 3 4 range
A 128.4
(-3)
A 		8877.6
(3)
P 		8426.6
(2.7)
P 		74.1
(-3)
A 		8804
B 159.7
(-3)
A 		7749.5
(2.1)
P 		9043.1
(3)
P 		156.2
(-3)
A 		8887
C 210.1
(-3)
A 		7318.1
(2.9)
P 		7472.7
(3)
P 		203.7
(-3)
A 		7269
avr 166.1
(-3) 		7981.7
(2.8) 		8314.1
(3) 		144.7
(-3) 		8169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4196
1 2 3 4 5 6 range
A 286.4
(-3)
P 		3449.6
(1.8)
P 		3814
(2.3)
P 		4265.2
(3)
P 		4275.8
(3)
P 		3111.2
(1.2)
P 		3989
B 244.2
(-3)
P 		3263.1
(1.8)
P 		3933.9
(2.9)
P 		3989.1
(3)
P 		3803.4
(2.7)
P 		3263.8
(1.8)
P 		3745
C 283.6
(-3)
P 		3852.8
(2.2)
P 		4130
(2.6)
P 		4440.1
(3)
P 		3083.3
(1)
P 		3133.6
(1.1)
P 		4157
avr 271.4
(-3) 		3521.8
(1.9) 		3959.3
(2.6) 		4231.5
(3) 		3720.8
(2.2) 		3169.5
(1.4) 		3960
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2594
1 2 3 4 range
A 46.7
(-2.9)
A 		1927.1
(2.2)
P 		2202.9
(3)
P 		10.9
(-3)
A 		2192
B 50.9
(-2.9)
A 		1913.9
(2.5)
P 		2095.5
(3)
P 		33.3
(-3)
A 		2062
C 69.5
(-2.9)
A 		2104
(1.8)
P 		2604.5
(3)
P 		34.3
(-3)
A 		2570
avr 55.7
(-2.9) 		1981.7
(2.2) 		2301
(3) 		26.2
(-3) 		2275

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1493
1 2 3 4 5 6 range
A 52.8
(-3)
P 		1226.9
(2.7)
P 		830.6
(0.8)
P 		1081.8
(2)
P 		1290.3
(3)
P 		757.5
(0.4)
P 		1238
B 85
(-3)
P 		1086.2
(1.1)
P 		955.3
(0.6)
P 		1056.6
(1)
P 		1545.3
(3)
P 		1217.1
(1.7)
P 		1460
C 105.1
(-3)
P 		1175.6
(3)
P 		1051.2
(2.3)
P 		892.9
(1.4)
P 		997.7
(2)
P 		1049
(2.3)
P 		1071
avr 81
(-3) 		1162.9
(2.4) 		945.7
(1.3) 		1010.4
(1.7) 		1277.8
(3) 		1007.9
(1.6) 		1197
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185646_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17010
Sequence Type Exemplar sequence
Representative Public ID T01B10.2 NCBI
Target Description T01B10.2 /REP_DB=WormBase Gene ID /WP=CE12886 /TR=O02153 /GB=AAB52859.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077040(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077040 NCBI Caenorhabditis elegans GRounDhog (hedgehog-like family) family member (grd-14) (grd-14) mRNA, complete cds. 11/11 None
GENEFINDER00000012638 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8499653:8500858:1 11/11 None
SNAP00000012635 ENSEMBL cdna:SNAP chromosome:CEL140:X:8499975:8500858:1 9/11 None
T01B10.2 ENSEMBL cdna:known chromosome:CEL140:X:8499601:8500858:1 gene:T01B10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8499568-8500857(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grd-14
Ensembl T01B10.2
Entrez Gene 187934 Entrez gene
SwissProt O02153 EMBL-EBI
WormBase CE07456 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52859 Groundhog (hedgehog-like family) protein 14 [Caenorhabditis elegans] ref|NP_509441.2| GRounDhog (hedgehog-like family) family member (grd-14) [Caenorhabditis elegans] 2.0E-76
blast AAB52859 Groundhog (hedgehog-like family) protein 14 [Caenorhabditis elegans] ref|NP_509441.2| GRounDhog (hedgehog-like family) family member (grd-14) [Caenorhabditis elegans] 9.0E-76
blast CAE74910 Hypothetical protein CBG22788 [Caenorhabditis briggsae] 3.0E-61
blast CAE74910 Hypothetical protein CBG22788 [Caenorhabditis briggsae] 1.0E-60
blast AAP40518 Groundhog (hedgehog-like family) protein 13 [Caenorhabditis elegans] ref|NP_001023422.1| GRounDhog (hedgehog-like family) family member (grd-13) [Caenorhabditis elegans] 1.0E-55
Sequence
Target Sequence 
>C. ELEGANS:185646_AT
aaaaggtttcattgtcagcctccgtcttacagaagtcacaaaatttgaaatggtgaagct
tcttcttttcgtcgccatctcgtctgccacagtatttgctgcctatgatgacctcccaaa
gccagctgatccatatgagccaccaggttatgagccagaggcatgcccagacaagtactc
taaaatcattactcaacttcgttccgatctcggaaaagacgtctccagcatcaagttcac
aaaccagttggggtcccgagttcagaaagcatttggatctgctcacgagattatgatggg
accgagtgctccaactcttaaaactaacttcaacggaactatctgccaacatgcaggaga
cgatggattcaactacgttgtctacccatccccaggaaaatatgacattaacaacgtcgc
tgtagaagaatactttgagaagttcgccgagttcgctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAGGTTTCATTGTCAGCCTCCGT 235 133 369 Antisense
AGCCTCCGTCTTACAGAAGTCACAA 688 83 385 Antisense
CCATCTCGTCTGCCACAGTATTTGC 477 271 443 Antisense
AGCCAGCTGATCCATATGAGCCACC 187 83 488 Antisense
GAGCCACCAGGTTATGAGCCAGAGG 450 385 505 Antisense
GAGGCATGCCCAGACAAGTACTCTA 233 405 526 Antisense
ATCATTACTCAACTTCGTTCCGATC 441 29 553 Antisense
AAAAGACGTCTCCAGCATCAAGTTC 429 131 582 Antisense
ATGATGGGACCGAGTGCTCCAACTC 513 45 661 Antisense
GATTCAACTACGTTGTCTACCCATC 77 429 734 Antisense
TTGAGAAGTTCGCCGAGTTCGCTGC 120 653 803 Antisense
Sequence Source Affymetrix Proprietary Database