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[185644_at]

Description Y53F4B.18 /REP_DB=WormBase Gene ID /WP=CE22408 /TR=Q9NAB7 /GB=CAB61089.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 23      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 36.4
(3)
A 		8.3
(-3)
A 		13.2
(-2)
A 		20.4
(-0.4)
A 		28
B 67.2
(3)
A 		11.8
(-2.6)
A 		7.6
(-3)
A 		12.5
(-2.5)
A 		60
C 46.2
(-0.7)
A 		10.9
(-3)
A 		11.4
(-3)
A 		102.8
(3)
A 		92
avr 49.9
(3) 		10.3
(-3) 		10.7
(-2.9) 		45.2
(2.3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 8.7
(3)
A 		8.5
(2.6)
A 		6.8
(-0.5)
A 		5.4
(-3)
A 		6.3
(-1.4)
A 		6.2
(-1.5)
A 		3
B 36
(3)
A 		12.5
(-2)
A 		8.5
(-2.9)
A 		8
(-3)
A 		13.1
(-1.9)
A 		13
(-1.9)
A 		28
C 11.6
(-2.1)
A 		16.2
(-1.5)
A 		12
(-2.1)
A 		51.4
(3)
A 		4.7
(-3)
A 		5.7
(-2.9)
A 		47
avr 18.8
(1.8) 		12.4
(-1.1) 		9.1
(-2.5) 		21.6
(3) 		8
(-3) 		8.3
(-2.9) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 13.3
(3)
A 		1.8
(-3)
A 		3.4
(-2.2)
A 		3
(-2.4)
A 		12
B 21.4
(3)
A 		2.9
(-2.7)
A 		1.8
(-3)
A 		2.7
(-2.7)
A 		20
C 15.3
(2.2)
A 		3.1
(-3)
A 		4
(-2.6)
A 		17.3
(3)
A 		14
avr 16.7
(3) 		2.6
(-3) 		3.1
(-2.8) 		7.7
(-0.8) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 1.6
(-2.3)
A 		3
(3)
A 		1.5
(-2.6)
A 		1.4
(-3)
A 		1.9
(-1.1)
A 		1.5
(-2.6)
A 		2
B 12.5
(3)
A 		4.2
(-1.8)
A 		2.1
(-3)
A 		2.1
(-3)
A 		5.3
(-1.2)
A 		4.9
(-1.4)
A 		10
C 4.3
(-1.1)
A 		5
(-0.6)
A 		3.1
(-1.9)
A 		10.3
(3)
A 		1.5
(-3)
A 		1.9
(-2.7)
A 		9
avr 6.1
(3) 		4.1
(-0.1) 		2.2
(-3) 		4.6
(0.7) 		2.9
(-1.9) 		2.8
(-2.1) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185644_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3837
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.18 NCBI
Target Description Y53F4B.18 /REP_DB=WormBase Gene ID /WP=CE22408 /TR=Q9NAB7 /GB=CAB61089.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064702(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064702 NCBI Caenorhabditis elegans Y53F4B.18 (Y53F4B.18) mRNA, complete cds. 11/11 None
GENEFINDER00000043149 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15064581:15071556:-1 10/11 None
Y53F4B.18 ENSEMBL cdna:known chromosome:CEL140:II:15064581:15071556:-1 gene:Y53F4B.18 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043051 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15064577-15071553(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.18
Entrez Gene 190217 Entrez gene
SwissProt Q9NAB7 EMBL-EBI
WormBase CE22408 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1637576_S_AT dm
DROSGENOME1:153538_AT dm
HU35KSUBC:RC_W67536_AT hypothetical protein FLJ31204 hs
U133_X3P:HS.57876.0.A1_3P_AT hypothetical protein FLJ31204 hs
HG-U133_PLUS_2:230792_AT hypothetical protein FLJ31204 hs
HG-U133B:230792_AT hypothetical protein FLJ31204 hs
HG-U95B:47313_AT hypothetical protein FLJ31204 hs
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4040 amidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB61089 Hypothetical protein Y53F4B.18 [Caenorhabditis elegans] ref|NP_497103.1| Y53F4B.18 [Caenorhabditis elegans] 0.0
blast CAE73454 Hypothetical protein CBG20903 [Caenorhabditis briggsae] 0.0
blast AAM68671 CG8839-PE, isoform E [Drosophila melanogaster] gb|AAM68670.1| CG8839-PD, isoform D [Drosophila melanogaster] gb|AAM68669.1| CG8839-PC, isoform C [Drosophila melanogaster] gb|AAM68668.1| CG8839-PA, isoform A [Drosophila melanogaster] ref|NP_725139.1| CG8839-PE, isoform E [Drosophila melanogaster] ref|NP_725138.1| CG8839-PD, isoform D [Drosophila melanogaster] ref|NP_725137.1| CG8839-PC, isoform C [Drosophila melanogaster] ref|NP_610764.1| CG8839-PA, isoform A [Drosophila melanogaster] gb|AAL28624.1| LD05247p [Drosophila melanogaster] 4.0E-88
blast AAM68671 CG8839-PE, isoform E [Drosophila melanogaster] gb|AAM68670.1| CG8839-PD, isoform D [Drosophila melanogaster] gb|AAM68669.1| CG8839-PC, isoform C [Drosophila melanogaster] gb|AAM68668.1| CG8839-PA, isoform A [Drosophila melanogaster] ref|NP_725139.1| CG8839-PE, isoform E [Drosophila melanogaster] ref|NP_725138.1| CG8839-PD, isoform D [Drosophila melanogaster] ref|NP_725137.1| CG8839-PC, isoform C [Drosophila melanogaster] ref|NP_610764.1| CG8839-PA, isoform A [Drosophila melanogaster] gb|AAL28624.1| LD05247p [Drosophila melanogaster] 7.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR000120 EMBL-EBI Amidase 7.8E-59
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497103.1 1 12-31
Y53F4B.18 1 12-31
GENEFINDER00000043149 1 12-31
Sequence
Target Sequence 
>C. ELEGANS:185644_AT
gtcgcccaaggaatcgaacttccctccgccaagcacgcaatggaattctttacactttca
atgcacgaagacactacagaccccgcatttaataaactaatgctctgtacaaaaggaacc
aaaggagaagtgaattgtttcactgaaatctttaaatatatggctggaaattcgaatcat
acactgtcgggtattgtggctggaattattgattcacgggatccgccgttctctgaagct
cacacgaagaacctgctttataagagagatcggctgaagcgacaggttaaagaactactc
ggcaccgacggtatcctgctcttcccaagttggccatgcacagccatgtatcacaatgag
cccatccttgctccgttcaacttctgctacacagctctctggaatgtgctctccgtcccc
gttgtccaatgtccgctgggactcgattcccgtgggcttccactcggcgtacaggtcatc
ggcaatcaatacactgaccgtaatctcattgcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGCCCAAGGAATCGAACTTCCCT 593 473 1051 Antisense
CACTACAGACCCCGCATTTAATAAA 543 195 1122 Antisense
TATTGATTCACGGGATCCGCCGTTC 611 659 1257 Antisense
CCGTTCTCTGAAGCTCACACGAAGA 154 265 1276 Antisense
AGAACTACTCGGCACCGACGGTATC 376 71 1341 Antisense
CTTCCCAAGTTGGCCATGCACAGCC 260 217 1371 Antisense
GCCATGTATCACAATGAGCCCATCC 475 281 1393 Antisense
CACAGCTCTCTGGAATGTGCTCTCC 642 195 1440 Antisense
CGTTGTCCAATGTCCGCTGGGACTC 697 245 1470 Antisense
TCGGCGTACAGGTCATCGGCAATCA 311 607 1514 Antisense
TACACTGACCGTAATCTCATTGCGA 430 643 1540 Antisense
Sequence Source Affymetrix Proprietary Database