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[185635_at]

Description F09C12.3 /REP_DB=WormBase Gene ID /WP=CE01902 /TR=Q10053 /GB=AAA68344.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 32      6grp_total > 33      4grp_total > 47      6grp_cgc > 90      6grp_high1_sub > 02_03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 47.7
(1.6)
P 		16.8
(-3)
A 		32.8
(-0.6)
A 		57.2
(3)
A 		40
B 65.2
(3)
P 		42
(-0.4)
A 		57.5
(1.9)
P 		24.7
(-3)
A 		41
C 86.4
(3)
P 		39.8
(-1.4)
A 		25.3
(-2.8)
A 		23.5
(-3)
A 		63
avr 66.4
(3) 		32.9
(-3) 		38.5
(-2) 		35.1
(-2.6) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 51.8
(2.3)
A 		21.1
(-3)
A 		56.1
(3)
P 		22.3
(-2.8)
P 		42
(0.6)
A 		42.9
(0.7)
A 		35
B 50.5
(1.3)
P 		19.5
(-3)
A 		56.7
(2.2)
P 		62.8
(3)
P 		51.6
(1.4)
P 		27.6
(-1.9)
P 		43
C 36.3
(0.7)
P 		56.6
(3)
P 		22.8
(-0.7)
A 		21.8
(-0.9)
A 		33.1
(0.4)
A 		2.5
(-3)
A 		54
avr 46.2
(3) 		32.4
(-0.8) 		45.2
(2.7) 		35.6
(0.1) 		42.2
(1.9) 		24.3
(-3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 17.4
(3)
P 		3.7
(-3)
A 		8.6
(-0.9)
A 		8.4
(-0.9)
A 		14
B 20.8
(3)
P 		10.4
(-1)
A 		13.3
(0.1)
P 		5.3
(-3)
A 		16
C 28.6
(3)
P 		11.4
(-1.2)
A 		8.8
(-1.8)
A 		4
(-3)
A 		25
avr 22.3
(3) 		8.5
(-2) 		10.2
(-1.4) 		5.9
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 9.5
(0.3)
A 		7.5
(-1.5)
A 		12.2
(2.6)
P 		5.7
(-3)
P 		12.7
(3)
A 		10.4
(1)
A 		7
B 17.6
(1.6)
P 		6.5
(-3)
A 		13.8
(0)
P 		16.6
(1.2)
P 		20.9
(3)
P 		10.3
(-1.4)
P 		14
C 13.5
(1.6)
P 		17.3
(3)
P 		5.8
(-1.2)
A 		4.4
(-1.7)
A 		10.7
(0.6)
A 		0.9
(-3)
A 		16
avr 13.5
(2) 		10.4
(-0.5) 		10.6
(-0.3) 		8.9
(-1.7) 		14.8
(3) 		7.2
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185635_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5318
Sequence Type Exemplar sequence
Representative Public ID F09C12.3 NCBI
Target Description F09C12.3 /REP_DB=WormBase Gene ID /WP=CE01902 /TR=Q10053 /GB=AAA68344.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062545(11), NM_001026742(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062545 NCBI Caenorhabditis elegans MAP Kinase family member (mpk-2) (mpk-2) mRNA, complete cds. 11/11 A 
NM_001026742 NCBI Caenorhabditis elegans MAP Kinase family member (mpk-2) (mpk-2) mRNA, complete cds. 10/11 None
SNAP00000009137 ENSEMBL cdna:SNAP chromosome:CEL140:II:5107771:5108791:1 11/11 None
GENEFINDER00000009154 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5107911:5114108:1 10/11 None
C04G6.1c ENSEMBL cdna:known chromosome:CEL140:II:5104100:5108791:1 gene:C04G6.1 11/11 A 
C04G6.1a ENSEMBL cdna:known chromosome:CEL140:II:5104446:5108854:1 gene:C04G6.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173880 3 2 NM_001026742 185635_at (10)
NM_062545 185635_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5107960-5108789(+) 100.0 100.0
Public Domain and Genome References
Ensembl C04G6.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA98016 Map kinase protein 2, isoform a [Caenorhabditis elegans] ref|NP_494946.2| MAP Kinase family member (mpk-2) [Caenorhabditis elegans] 0.0
blast AAT81209 Map kinase protein 2, isoform c [Caenorhabditis elegans] ref|NP_001021913.1| MAP Kinase family member (mpk-2) [Caenorhabditis elegans] 0.0
blast CAE59045 Hypothetical protein CBG02327 [Caenorhabditis briggsae] 0.0
blast T15983 hypothetical protein F09C12.2 - Caenorhabditis elegans 0.0
blast AAN63410 Hypothetical protein F09C12.2 [Caenorhabditis elegans] ref|NP_872069.1| F09C12.2 [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-70
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-72
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-72
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 4.0E-75
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 1.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 6.0E-83
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-86
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-86
Sequence
Target Sequence 
>C. ELEGANS:185635_AT
tggaattaattggccattggatgttacaagtatttctagtgctccacctatgttatctgg
cggcatccctccttcaaaaaaatcgaaaaatcatctggatcgaagaaacatcgaccgaat
acgagaaggatacagtgagaaacgacgatttattccgaaaattcgagacaagcgagctaa
gtgatagttcatcaaaacttgatgacaaagatcgaatagtgagattgcgaaagtctcgac
aggagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAATTAATTGGCCATTGGATGTT 242 533 14 Antisense
TGTTACAAGTATTTCTAGTGCTCCA 427 561 35 Antisense
TAGTGCTCCACCTATGTTATCTGGC 685 653 50 Antisense
TCCACCTATGTTATCTGGCGGCATC 304 589 56 Antisense
GGCATCCCTCCTTCAAAAAAATCGA 110 535 75 Antisense
TCTGGATCGAAGAAACATCGACCGA 612 607 107 Antisense
GAAACATCGACCGAATACGAGAAGG 656 355 118 Antisense
GTGAGAAACGACGATTTATTCCGAA 331 481 148 Antisense
CCGAAAATTCGAGACAAGCGAGCTA 647 195 168 Antisense
AAGCGAGCTAAGTGATAGTTCATCA 156 149 183 Antisense
GATTGCGAAAGTCTCGACAGGAGTT 512 433 236 Antisense
Sequence Source Affymetrix Proprietary Database