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[185631_at]

Description C01B12.5 /REP_DB=WormBase Gene ID /WP=CE07793 /TR=O17205 /GB=AAB70978.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 41      6grp_total > 33      4grp_total > 62      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 126.8
(3)
P 		87.6
(-0.4)
A 		83.8
(-0.7)
A 		56.8
(-3)
A 		70
B 130
(3)
P 		85.5
(-1.4)
P 		90.8
(-0.8)
A 		68.7
(-3)
P 		61
C 130.1
(3)
P 		73.8
(-0)
A 		82.4
(0.4)
P 		18.4
(-3)
A 		112
avr 129
(3) 		82.3
(-0.5) 		85.7
(-0.2) 		48
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 92.6
(2.4)
P 		102.5
(3)
P 		76.9
(1.4)
P 		80.8
(1.6)
P 		6.4
(-3)
A 		19.6
(-2.2)
A 		96
B 99.5
(2.3)
P 		62.2
(-0.7)
P 		107.6
(3)
P 		89.6
(1.5)
P 		39.4
(-2.6)
A 		34.3
(-3)
A 		73
C 80.2
(0.8)
P 		76.4
(0.5)
P 		102.6
(3)
P 		40.9
(-3)
A 		49.7
(-2.1)
A 		78.9
(0.7)
P 		62
avr 90.8
(2.5) 		80.4
(1.6) 		95.7
(3) 		70.4
(0.6) 		31.8
(-3) 		44.3
(-1.8) 		64
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 46.1
(3)
P 		19
(-1.3)
A 		21.9
(-0.8)
A 		8.3
(-3)
A 		38
B 41.4
(3)
P 		21.1
(-1.5)
P 		21
(-1.6)
A 		14.6
(-3)
P 		27
C 43.1
(3)
P 		21.2
(-0.3)
A 		28.7
(0.8)
P 		3.1
(-3)
A 		40
avr 43.5
(3) 		20.4
(-1) 		23.9
(-0.4) 		8.7
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 17.1
(-0.4)
P 		36.4
(3)
P 		16.7
(-0.4)
P 		20.5
(0.2)
P 		1.9
(-3)
A 		4.8
(-2.5)
A 		35
B 34.6
(3)
P 		20.7
(-0.8)
P 		26.1
(0.7)
P 		23.7
(-0)
P 		16
(-2.1)
A 		12.8
(-3)
A 		22
C 29.7
(3)
P 		23.3
(1.2)
P 		26.1
(2)
P 		8.2
(-3)
A 		16.1
(-0.8)
A 		26.4
(2.1)
P 		22
avr 27.1
(3) 		26.8
(2.9) 		23
(1.4) 		17.5
(-0.6) 		11.3
(-3) 		14.7
(-1.7) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185631_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5948
Sequence Type Exemplar sequence
Representative Public ID C01B12.5 NCBI
Target Description C01B12.5 /REP_DB=WormBase Gene ID /WP=CE07793 /TR=O17205 /GB=AAB70978.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061230(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061230 NCBI Caenorhabditis elegans C01B12.5 (C01B12.5) mRNA, complete cds. 11/11 None
SNAP00000043174 ENSEMBL cdna:SNAP chromosome:CEL140:II:9485:11540:-1 11/11 None
GENEFINDER00000043181 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9485:11826:-1 11/11 None
C01B12.5 ENSEMBL cdna:known chromosome:CEL140:II:9808:11826:-1 gene:C01B12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9807-11826(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C01B12.5
Entrez Gene 182063 Entrez gene
SwissProt O17205 EMBL-EBI
WormBase CE07793 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1634031_AT Bestrophin 2 dm
DROSGENOME1:148276_AT Bestrophin 2 dm
Protein Similarities
Method ID Description E-Value
blast AAB70978 Hypothetical protein C01B12.5 [Caenorhabditis elegans] ref|NP_493631.1| C01B12.5 [Caenorhabditis elegans] 0.0
blast CAE62860 Hypothetical protein CBG07043 [Caenorhabditis briggsae] 0.0
blast CAE62861 Hypothetical protein CBG07044 [Caenorhabditis briggsae] 1.0E-136
Protein Domains
Database ID Description E-Value
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493631.1 3 31-53,68-90,221-243
C01B12.5 3 31-53,68-90,221-243
GENEFINDER00000043181 3 31-53,68-90,221-243
Sequence
Target Sequence 
>C. ELEGANS:185631_AT
atgttcgacgccgatcgagatcgtttggtgatgatgatgcatcgtctttttcaactgaaa
atacaagaacgcaaagtaacgcttcgatgttgccacgacatatgtggcaacaaccgcgta
acaaaatttctgcagctatcgcaaaattttataaatcagaagctgaaccgaggagagcta
gtgtatgcgtagcagctttgaaccttaaccatcgtcgcccatcaacagatttacgtgaaa
ttgagccaatagaaaacacagatgatgttgtgataaatgtgcctgcacttgttcaagaac
gtattcgaatagcaaaacaacaggcagtaaaaatagaaattccggaagaacctctgaaaa
ttactgcttctgctgaaggaaaatcggcgatttccccgtctgccaacaatgtaaaatggt
tcgttgaagaaatgccagtaatcgaagaagaagaagaaagactgcacaggaaaacgccga
ga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTCGACGCCGATCGAGATCGTT 126 47 1151 Antisense
GATGCATCGTCTTTTTCAACTGAAA 677 411 1186 Antisense
AAGTAACGCTTCGATGTTGCCACGA 438 157 1224 Antisense
GACATATGTGGCAACAACCGCGTAA 294 367 1247 Antisense
GTAACAAAATTTCTGCAGCTATCGC 675 461 1268 Antisense
GAACCGAGGAGAGCTAGTGTATGCG 10 347 1315 Antisense
GCAGCTTTGAACCTTAACCATCGTC 389 313 1342 Antisense
ATCGTCGCCCATCAACAGATTTACG 616 35 1361 Antisense
GATAAATGTGCCTGCACTTGTTCAA 119 423 1422 Antisense
GAACCTCTGAAAATTACTGCTTCTG 155 347 1498 Antisense
AAGACTGCACAGGAAAACGCCGAGA 697 153 1608 Antisense
Sequence Source Affymetrix Proprietary Database