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[185628_at]

Description F21D12.3 /REP_DB=WormBase Gene ID /WP=CE02668 /TR=Q19676 /GB=AAC70884.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 38      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 40      6grp_total > 37      4grp_total > 22      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 912
1 2 3 4 range
A 1321.1
(2.4)
P 		825.5
(-1.3)
P 		592.5
(-3)
P 		1399.3
(3)
P 		807
B 1358.5
(3)
P 		893.7
(-0.8)
P 		617.5
(-3)
P 		1244.8
(2.1)
P 		741
C 1372.4
(2)
P 		903
(-1.5)
P 		705.6
(-3)
P 		1504.5
(3)
P 		799
avr 1350.7
(2.7) 		874.1
(-1.1) 		638.5
(-3) 		1382.9
(3) 		744

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 535
1 2 3 4 5 6 range
A 961.3
(3)
P 		731
(0.4)
P 		440.1
(-2.8)
P 		426.7
(-3)
P 		624.1
(-0.8)
P 		773.5
(0.9)
P 		535
B 930
(3)
P 		753.9
(0.4)
P 		596
(-1.9)
P 		552.3
(-2.6)
P 		523.6
(-3)
P 		841.6
(1.7)
P 		406
C 880.2
(3)
P 		847.1
(2.5)
P 		534.5
(-2.1)
P 		514.4
(-2.4)
P 		471.1
(-3)
P 		815.1
(2)
P 		409
avr 923.8
(3) 		777.3
(0.9) 		523.5
(-2.6) 		497.8
(-3) 		539.6
(-2.4) 		810.1
(1.4) 		426
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 338
1 2 3 4 range
A 481
(3)
P 		179.2
(-2.6)
P 		154.9
(-3)
P 		204.8
(-2.1)
P 		326
B 433.2
(3)
P 		220.7
(-1.4)
P 		143.1
(-3)
P 		265.3
(-0.5)
P 		290
C 454.3
(3)
P 		259.6
(-2.6)
P 		245.9
(-3)
P 		253.7
(-2.8)
P 		208
avr 456.2
(3) 		219.8
(-2.2) 		181.3
(-3) 		241.3
(-1.7) 		275

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 177.2
(-0)
P 		260
(3)
P 		95.8
(-3)
P 		108.2
(-2.5)
P 		188.3
(0.4)
P 		188.3
(0.4)
P 		164
B 323.6
(3)
P 		250.9
(0.6)
P 		144.7
(-3)
P 		146.3
(-2.9)
P 		212.7
(-0.7)
P 		313.8
(2.7)
P 		179
C 326.3
(3)
P 		258.5
(1.2)
P 		136
(-2.1)
P 		103.4
(-3)
P 		152.4
(-1.7)
P 		272.8
(1.6)
P 		223
avr 275.7
(3) 		256.5
(2.3) 		125.5
(-2.8) 		119.3
(-3) 		184.5
(-0.5) 		258.3
(2.3) 		156
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185628_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5544
Sequence Type Exemplar sequence
Representative Public ID F21D12.3 NCBI
Target Description F21D12.3 /REP_DB=WormBase Gene ID /WP=CE02668 /TR=Q19676 /GB=AAC70884.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063131(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063131 NCBI Caenorhabditis elegans F21D12.3 (F21D12.3) mRNA, complete cds. 11/11 None
SNAP00000020729 ENSEMBL cdna:SNAP chromosome:CEL140:II:7320547:7322868:-1 11/11 None
GENEFINDER00000020734 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7320342:7322832:-1 11/11 None
F21D12.3 ENSEMBL cdna:known chromosome:CEL140:II:7319829:7322843:-1 gene:F21D12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7320543-7322825(-) 97.17 100.0
Public Domain and Genome References
Gene Title
Ensembl F21D12.3
Entrez Gene 174201 Entrez gene
SwissProt Q19676 EMBL-EBI
WormBase CE32633 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6865 amino acid transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5279 amino acid-polyamine transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC70884 Hypothetical protein F21D12.3 [Caenorhabditis elegans] ref|NP_495532.2| F21D12.3 [Caenorhabditis elegans] 0.0
blast CAE67596 Hypothetical protein CBG13138 [Caenorhabditis briggsae] 0.0
blast B88206 protein F21D12.3 [imported] - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 6.0E-44
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 6.0E-44
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495532.2 11 47-69,74-96,133-155,165-187,194-216,226-248,269-291,311-333,346-368,378-397,425-447
F21D12.3 11 47-69,74-96,133-155,165-187,194-216,226-248,269-291,311-333,346-368,378-397,425-447
SNAP00000020729 11 59-81,86-108,145-167,177-199,206-228,238-260,281-303,323-345,358-380,390-409,437-459
GENEFINDER00000020734 11 47-69,74-96,133-155,165-187,194-216,226-248,269-291,311-333,346-368,378-397,425-447
Sequence
Target Sequence 
>C. ELEGANS:185628_AT
tttgctggattcttgggtgttgtcattctttatcttccactctgtatatttgcatttgtg
gtttatggagattcaatgactgattcagttatttacagtattcaaagtccttcattgcag
cttcttgccaatttgatgatctcatttcattgtataatgactcttgtaattgtaattaat
ccattaaatcaagaagttgaacattatgcaaagatctcacatgcctttggaattggaaga
gttattactagaactattgttctattcttggttctttttgtcgcactgactgttccagat
ttccaacctgttatgaatcttgttggagcttcaacaattccaatgggatgtgccgtcctt
ccttcacttttctacttgtactcagaagctgccacagaagaggaatggaggaaaggaaag
attccaacactcagagaagtattggatcggacagacaaaactgttttgattatcaacctt
atcatcatcttcggagccattcttggtggagtgctcggatcatatcaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGCTGGATTCTTGGGTGTTGTCA 387 669 901 Antisense
GTGTTGTCATTCTTTATCTTCCACT 402 485 917 Antisense
ATCTTCCACTCTGTATATTTGCATT 470 31 932 Antisense
CAAAGTCCTTCATTGCAGCTTCTTG 347 193 1003 Antisense
AGATCTCACATGCCTTTGGAATTGG 339 63 1112 Antisense
TATTCTTGGTTCTTTTTGTCGCACT 585 661 1163 Antisense
GTCGCACTGACTGTTCCAGATTTCC 221 475 1180 Antisense
AATTCCAATGGGATGTGCCGTCCTT 131 179 1236 Antisense
CCTTCACTTTTCTACTTGTACTCAG 182 267 1261 Antisense
TCAACCTTATCATCATCTTCGGAGC 320 631 1373 Antisense
GGTGGAGTGCTCGGATCATATCAAG 435 499 1405 Antisense
Sequence Source Affymetrix Proprietary Database