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[185620_at]

Description F08D12.8 /REP_DB=WormBase Gene ID /WP=CE09241 /TR=P91231 /GB=AAB37932.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 56      4grp_total > 01      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 20.8
(-3)
A 		32.3
(-2)
A 		51.7
(-0.3)
A 		88.9
(3)
A 		68
B 46.9
(1.8)
A 		11.7
(-2.7)
A 		9.2
(-3)
A 		56.1
(3)
A 		47
C 29.5
(-1.2)
A 		14.1
(-3)
A 		28.3
(-1.4)
A 		65.9
(3)
A 		52
avr 32.4
(-1.5) 		19.4
(-3) 		29.7
(-1.8) 		70.3
(3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 10.6
(-2.9)
A 		61.2
(3)
A 		10.1
(-3)
A 		15.9
(-2.3)
A 		26.6
(-1.1)
A 		54.4
(2.2)
A 		51
B 33.3
(1.8)
A 		31.9
(1.6)
A 		7.2
(-3)
A 		13
(-1.9)
A 		19.4
(-0.7)
A 		39.5
(3)
A 		32
C 6.3
(-3)
A 		41.8
(1.9)
A 		49.4
(3)
A 		31.7
(0.5)
A 		20.9
(-1)
A 		41.6
(1.9)
A 		43
avr 16.7
(-3) 		45
(3) 		22.2
(-1.8) 		20.2
(-2.3) 		22.3
(-1.8) 		45.2
(3) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 7.6
(-2.4)
A 		7
(-3)
A 		13.5
(3)
A 		13
(2.5)
A 		7
B 15
(3)
A 		2.9
(-2.6)
A 		2.1
(-3)
A 		11.9
(1.6)
A 		13
C 9.8
(1.9)
A 		4.1
(-3)
A 		9.9
(2)
A 		11.1
(3)
A 		7
avr 10.8
(2) 		4.7
(-3) 		8.5
(0.1) 		12
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 1.9
(-3)
A 		21.8
(3)
A 		2.2
(-2.9)
A 		4
(-2.4)
A 		8
(-1.2)
A 		13.3
(0.4)
A 		20
B 11.6
(1.6)
A 		10.6
(1.1)
A 		1.8
(-3)
A 		3.4
(-2.3)
A 		7.9
(-0.2)
A 		14.7
(3)
A 		13
C 2.4
(-3)
A 		12.7
(2.4)
A 		12.6
(2.3)
A 		6.4
(-0.9)
A 		6.8
(-0.7)
A 		13.9
(3)
A 		12
avr 5.3
(-2.6) 		15
(3) 		5.5
(-2.5) 		4.6
(-3) 		7.6
(-1.3) 		14
(2.4) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185620_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4484
Sequence Type Exemplar sequence
Representative Public ID F08D12.8 NCBI
Target Description F08D12.8 /REP_DB=WormBase Gene ID /WP=CE09241 /TR=P91231 /GB=AAB37932.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061956(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061956 NCBI Caenorhabditis elegans F08D12.8 (F08D12.8) mRNA, complete cds. 11/11 None
SNAP00000023714 ENSEMBL cdna:SNAP chromosome:CEL140:II:2767270:2770888:-1 11/11 None
F08D12.8 ENSEMBL cdna:known chromosome:CEL140:II:2769794:2770888:-1 gene:F08D12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2769792-2770887(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F08D12.8
Entrez Gene 184178 Entrez gene
SwissProt P91231 EMBL-EBI
WormBase CE09241 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37932 Hypothetical protein F08D12.8 [Caenorhabditis elegans] ref|NP_494357.1| F08D12.8 [Caenorhabditis elegans] 1.0E-174
blast AAB37932 Hypothetical protein F08D12.8 [Caenorhabditis elegans] ref|NP_494357.1| F08D12.8 [Caenorhabditis elegans] 1.0E-171
blast AAK68305 Hypothetical protein F08D12.7 [Caenorhabditis elegans] ref|NP_494356.1| F08D12.7 [Caenorhabditis elegans] 1.0E-150
blast CAE72417 Hypothetical protein CBG19579 [Caenorhabditis briggsae] 1.0E-110
blast AAK68304 Hypothetical protein F08D12.6 [Caenorhabditis elegans] ref|NP_494355.1| F08D12.6 [Caenorhabditis elegans] 5.0E-47
blast T25674 hypothetical protein F08D12.6 - Caenorhabditis elegans 5.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.0E-8
Pfam IPR004920 EMBL-EBI Protein of unknown function DUF263 3.3E-59
Pfam IPR004920 EMBL-EBI Protein of unknown function DUF263 12.0
Sequence
Target Sequence 
>C. ELEGANS:185620_AT
tttgctcggatactgttgtccagagtgtagtcgccaatgtccaaagcaatgatatatcct
ttaatttatcattcaacattgaaccgtccatgcaaaagcataaagtacttactcgacact
tcgaacgtgtttcattttccaaagatgatactagagaagctctcacgttaaatgatttat
tgatcaccaacgtctcccaactaaatatttccgctcatcatctgtctttcgaagagttga
acattttcatcaggcacttgattagtggatcgaatcggaggctcaaaattgtcggcattg
tttgtgctgaagcgaccaggaacatgcaaattttgttgaaaggaatttctcactcggtgt
actcggcggagctaaaaattgtggctcgaaacgaaaacgactaccaacttggtgatacta
tctatgtataccaaaattgtggaagaaggttggcgatgcagacgtactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGCTCGGATACTGTTGTCCAGAG 278 667 503 Antisense
TCCAGAGTGTAGTCGCCAATGTCCA 689 587 521 Antisense
ATTCAACATTGAACCGTCCATGCAA 292 11 573 Antisense
GTACTTACTCGACACTTCGAACGTG 653 455 607 Antisense
GATTTATTGATCACCAACGTCTCCC 479 425 676 Antisense
GTCTCCCAACTAAATATTTCCGCTC 561 467 694 Antisense
CGCTCATCATCTGTCTTTCGAAGAG 623 255 714 Antisense
GGAGGCTCAAAATTGTCGGCATTGT 360 513 779 Antisense
GAAAGGAATTTCTCACTCGGTGTAC 170 355 840 Antisense
CTCGGTGTACTCGGCGGAGCTAAAA 188 229 855 Antisense
GAAGGTTGGCGATGCAGACGTACTT 513 333 947 Antisense
Sequence Source Affymetrix Proprietary Database