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[185613_s_at]

Description T05B4.13 /REP_DB=WormBase Gene ID /WP=CE13217 /TR=O16422 /GB=AAC69061.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 26      4grp_total > 18      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 9
(-3)
A 		81.2
(3)
A 		9.7
(-2.9)
A 		79.1
(2.8)
A 		72
B 20.4
(-1.7)
A 		6.6
(-3)
A 		42.6
(0.3)
A 		72.2
(3)
A 		66
C 29.7
(-1.9)
A 		24.5
(-3)
A 		28.1
(-2.2)
A 		51.9
(3)
A 		27
avr 19.7
(-3) 		37.4
(-0.8) 		26.8
(-2.1) 		67.7
(3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 20.6
(-0.1)
A 		39.8
(3)
A 		33.7
(2)
A 		5.2
(-2.6)
A 		28.5
(1.2)
A 		3
(-3)
A 		37
B 45.7
(3)
A 		8.7
(-2.7)
A 		6.5
(-3)
A 		9.7
(-2.5)
A 		11.1
(-2.3)
A 		43.3
(2.6)
A 		39
C 44.4
(1.4)
A 		57.6
(3)
A 		7.6
(-3)
A 		27.2
(-0.6)
A 		10
(-2.7)
A 		14.9
(-2.1)
A 		50
avr 36.9
(3) 		35.4
(2.6) 		15.9
(-2.5) 		14
(-3) 		16.5
(-2.3) 		20.4
(-1.3) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 3.3
(-2.7)
A 		17.6
(3)
A 		2.5
(-3)
A 		11.6
(0.6)
A 		15
B 6.5
(-0.9)
A 		1.6
(-3)
A 		9.9
(0.6)
A 		15.4
(3)
A 		14
C 9.8
(3)
A 		7
(-3)
A 		9.8
(3)
A 		8.8
(0.9)
A 		3
avr 6.5
(-3) 		8.7
(-0.6) 		7.4
(-2) 		11.9
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 3.8
(-1.6)
A 		14.1
(3)
A 		7.3
(-0)
A 		1.3
(-2.7)
A 		8.6
(0.5)
A 		0.7
(-3)
A 		13
B 15.9
(2.9)
A 		2.9
(-2.5)
A 		1.6
(-3)
A 		2.6
(-2.6)
A 		4.5
(-1.8)
A 		16.1
(3)
A 		15
C 16.4
(2.5)
A 		17.6
(3)
A 		1.9
(-3)
A 		5.5
(-1.6)
A 		3.2
(-2.5)
A 		5
(-1.8)
A 		16
avr 12
(3) 		11.5
(2.7) 		3.6
(-2.7) 		3.1
(-3) 		5.4
(-1.4) 		7.3
(-0.2) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185613_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15522
Sequence Type Exemplar sequence
Representative Public ID T05B4.13 NCBI
Target Description T05B4.13 /REP_DB=WormBase Gene ID /WP=CE13217 /TR=O16422 /GB=AAC69061.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071750(11), NM_071749(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071750 NCBI Caenorhabditis elegans T05B4.4 (T05B4.4) mRNA, complete cds. 11/11 None
NM_071749 NCBI Caenorhabditis elegans T05B4.13 (T05B4.13) mRNA, complete cds. 11/11 A 
SNAP00000031066 ENSEMBL cdna:SNAP chromosome:CEL140:V:4126132:4127407:-1 11/11 None
SNAP00000031067 ENSEMBL cdna:SNAP chromosome:CEL140:V:4127681:4129415:1 11/11 None
GENEFINDER00000031076 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4127681:4129415:1 11/11 None
GENEFINDER00000031080 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4126132:4127348:-1 11/11 None
T05B4.13 ENSEMBL cdna:known chromosome:CEL140:V:4126132:4127024:-1 gene:T05B4.13 11/11 A 
T05B4.4 ENSEMBL cdna:known chromosome:CEL140:V:4127363:4129413:1 gene:T05B4.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4128288-4129415(+) 95.54 95.54
chrV:4126131-4127024(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl T05B4.13
T05B4.4
Entrez Gene 188100 Entrez gene
188108 Entrez gene
SwissProt O16422 EMBL-EBI
O16423 EMBL-EBI
WormBase CE13217 Wormbase
CE30826 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE65452 Hypothetical protein CBG10417 [Caenorhabditis briggsae] 1.0E-120
blast CAE65452 Hypothetical protein CBG10417 [Caenorhabditis briggsae] 1.0E-116
blast CAE65453 Hypothetical protein CBG10418 [Caenorhabditis briggsae] 1.0E-116
blast CAE65453 Hypothetical protein CBG10418 [Caenorhabditis briggsae] 1.0E-112
blast CAE65454 Hypothetical protein CBG10419 [Caenorhabditis briggsae] 1.0E-93
blast AAC69054 Hypothetical protein T05B4.3 [Caenorhabditis elegans] ref|NP_504152.1| T05B4.3 [Caenorhabditis elegans] 3.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.7E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.1E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.9E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.1E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.7E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.9E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.9E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.1E-14
Sequence
Target Sequence 
>C. ELEGANS:185613_S_AT
gactgctgctttggagacttgtgccaagacttgtggattgtgctgtcagacggatgcata
cagttgcaacccacgcctcgactgctcaacaatcaccactgctcaatgtaactctatcat
ctggagatccatcattgccaccgactgcccatctgcttgcggattctgtaacgagggagg
atgtgttgatgctcttgttgactgtgccactgatatctcaatctgcactacagttggaat
gcaagatttcgtgaatacctactgccaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTGCTGCTTTGGAGACTTGTGCC 570 375 18 Antisense
CTCAACAATCACCACTGCTCAATGT 702 227 102 Antisense
ACCACTGCTCAATGTAACTCTATCA 653 85 112 Antisense
TGTAACTCTATCATCTGGAGATCCA 239 559 124 Antisense
TATCATCTGGAGATCCATCATTGCC 563 659 132 Antisense
GGATGTGTTGATGCTCTTGTTGACT 671 513 196 Antisense
GTTGATGCTCTTGTTGACTGTGCCA 263 439 202 Antisense
TTGTTGACTGTGCCACTGATATCTC 87 707 212 Antisense
GCCACTGATATCTCAATCTGCACTA 509 279 223 Antisense
ATCTCAATCTGCACTACAGTTGGAA 184 33 232 Antisense
GATTTCGTGAATACCTACTGCCAAA 73 429 262 Antisense
Sequence Source Affymetrix Proprietary Database