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[185612_at]

Description T05B4.13 /REP_DB=WormBase Gene ID /WP=CE13217 /TR=O16422 /GB=AAC69061.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 02      4grp_total > 09      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 2.9
(-3)
A 		9.2
(3)
A 		6
(-0)
A 		7.2
(1.1)
A 		6
B 3.9
(-1.1)
A 		2.9
(-3)
A 		5.2
(1.3)
A 		6.1
(3)
A 		3
C 6.5
(-2.5)
A 		4.4
(-3)
A 		5.6
(-2.7)
A 		30.8
(3)
A 		26
avr 4.4
(-3) 		5.5
(-2.4) 		5.6
(-2.3) 		14.7
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 7.3
(2.9)
A 		4.2
(-1)
A 		2.8
(-2.8)
A 		7.4
(3)
A 		2.6
(-3)
A 		6.7
(2.1)
A 		5
B 3.8
(-2.6)
A 		26.3
(3)
A 		2.5
(-3)
A 		5.6
(-2.2)
A 		2.3
(-3)
A 		4.8
(-2.4)
A 		24
C 11.4
(3)
A 		5.1
(-1.8)
A 		9.7
(1.7)
A 		5.3
(-1.6)
A 		3.7
(-2.8)
A 		3.5
(-3)
A 		8
avr 7.5
(0.1) 		11.9
(3) 		5
(-1.6) 		6.1
(-0.9) 		2.9
(-3) 		5
(-1.6) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 1.1
(-2.4)
A 		2
(3)
A 		1.6
(0.6)
A 		1
(-3)
A 		1
B 1.3
(3)
A 		0.7
(-3)
A 		1.2
(2)
A 		1.3
(3)
A 		1
C 2.2
(-1.6)
A 		1.3
(-3)
A 		1.9
(-2.1)
A 		5.2
(3)
A 		4
avr 1.5
(-2) 		1.3
(-3) 		1.6
(-1.5) 		2.5
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1.3
(0.2)
A 		1.5
(1.2)
A 		0.6
(-3)
A 		1.9
(3)
A 		0.8
(-2.1)
A 		1.6
(1.6)
A 		1
B 1.3
(-2.5)
A 		8.7
(3)
A 		0.6
(-3)
A 		1.5
(-2.3)
A 		0.9
(-2.8)
A 		1.8
(-2.1)
A 		8
C 4.2
(3)
A 		1.5
(-2.2)
A 		2.5
(-0.3)
A 		1.1
(-3)
A 		1.2
(-2.8)
A 		1.2
(-2.8)
A 		3
avr 2.3
(-0.3) 		3.9
(3) 		1.2
(-2.6) 		1.5
(-2) 		1
(-3) 		1.5
(-2) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185612_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15522
Sequence Type Exemplar sequence
Representative Public ID T05B4.13 NCBI
Target Description T05B4.13 /REP_DB=WormBase Gene ID /WP=CE13217 /TR=O16422 /GB=AAC69061.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071749(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071749 NCBI Caenorhabditis elegans T05B4.13 (T05B4.13) mRNA, complete cds. 11/11 A 
SNAP00000031066 ENSEMBL cdna:SNAP chromosome:CEL140:V:4126132:4127407:-1 11/11 None
GENEFINDER00000031080 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4126132:4127348:-1 11/11 None
T05B4.13 ENSEMBL cdna:known chromosome:CEL140:V:4126132:4127024:-1 gene:T05B4.13 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4128288-4129415(+) 95.54 95.54
chrV:4126131-4127024(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T05B4.13
Entrez Gene 188108 Entrez gene
SwissProt O16422 EMBL-EBI
WormBase CE13217 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE65452 Hypothetical protein CBG10417 [Caenorhabditis briggsae] 1.0E-120
blast CAE65452 Hypothetical protein CBG10417 [Caenorhabditis briggsae] 1.0E-116
blast CAE65453 Hypothetical protein CBG10418 [Caenorhabditis briggsae] 1.0E-116
blast CAE65453 Hypothetical protein CBG10418 [Caenorhabditis briggsae] 1.0E-112
blast CAE65454 Hypothetical protein CBG10419 [Caenorhabditis briggsae] 1.0E-93
blast CAE65454 Hypothetical protein CBG10419 [Caenorhabditis briggsae] 6.0E-92
blast AAC69061 Hypothetical protein T05B4.13 [Caenorhabditis elegans] ref|NP_504150.1| T05B4.13 [Caenorhabditis elegans] 2.0E-77
blast CAE65452 Hypothetical protein CBG10417 [Caenorhabditis briggsae] 1.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.7E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.1E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.9E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.1E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.9E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.1E-14
Sequence
Target Sequence 
>C. ELEGANS:185612_AT
attgtgctgtcagacggatgcatacagttgcaacccacgcctcgactgctcaacaatcac
cactgctcaatgtaactctatcatctggagatccatcattgccaccgactgcccatctgc
ttgcggattctgtaacgagggaggatgtgttgatgctcttgttgactgtgccactgatat
ctcaatctgcactacagttggaatgcaagatttcgtgaatacctactgccaaagaacatg
tggaagatgcccatcatctactactgcatcatcaagctccacagcttcatcttcctccac
atgcacttcttataatgcggatagttcatctctctgttccgactgggcagccaatggatt
ctgcaccaatgctttctacacaagtgctcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGTGCTGTCAGACGGATGCATAC 699 3 54 Antisense
CTGTCAGACGGATGCATACAGTTGC 406 237 60 Antisense
AGATGCCCATCATCTACTACTGCAT 475 67 298 Antisense
TGCCCATCATCTACTACTGCATCAT 311 585 301 Antisense
CTACTACTGCATCATCAAGCTCCAC 289 225 311 Antisense
CTACTGCATCATCAAGCTCCACAGC 22 227 314 Antisense
CACATGCACTTCTTATAATGCGGAT 12 197 351 Antisense
TAATGCGGATAGTTCATCTCTCTGT 144 637 366 Antisense
CTGTTCCGACTGGGCAGCCAATGGA 703 237 387 Antisense
CCGACTGGGCAGCCAATGGATTCTG 305 261 392 Antisense
AATGCTTTCTACACAAGTGCTCAAA 640 163 421 Antisense
Sequence Source Affymetrix Proprietary Database