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[185598_at]

Description T10D4.11 /REP_DB=WormBase Gene ID /WP=CE20051 /TR=Q9TZE7 /GB=AAC68721.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 0      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 21.3
(-1.1)
A 		7.1
(-3)
A 		45.5
(2.1)
A 		52.2
(3)
A 		45
B 29.8
(1.7)
A 		12.1
(-2.4)
A 		35.4
(3)
A 		9.7
(-3)
A 		26
C 3.1
(-2.9)
A 		24.4
(-1.1)
A 		2.3
(-3)
A 		72.5
(3)
A 		70
avr 18.1
(-2.3) 		14.5
(-3) 		27.7
(-0.4) 		44.8
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 8.5
(-3)
A 		42.6
(3)
A 		17.2
(-1.5)
A 		13.1
(-2.2)
A 		17.8
(-1.4)
A 		25.7
(0)
A 		34
B 30.9
(0.7)
A 		23.1
(-0.3)
A 		48.1
(3)
A 		12.3
(-1.7)
A 		5.4
(-2.6)
A 		2.3
(-3)
A 		46
C 26.8
(-1.6)
A 		40.6
(0.8)
A 		18.4
(-3)
A 		53.9
(3)
A 		48.3
(2.1)
A 		19.4
(-2.8)
A 		36
avr 22.1
(-1.1) 		35.4
(3) 		27.9
(0.7) 		26.4
(0.2) 		23.8
(-0.6) 		15.8
(-3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 7.8
(0.6)
A 		1.5
(-3)
A 		11.9
(3)
A 		7.6
(0.5)
A 		10
B 9.5
(3)
A 		3
(-2.3)
A 		8.2
(1.9)
A 		2.1
(-3)
A 		7
C 1
(-2.9)
A 		7
(0.3)
A 		0.8
(-3)
A 		12.2
(3)
A 		11
avr 6.1
(0.9) 		3.8
(-3) 		7
(2.5) 		7.3
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 1.6
(-3)
A 		15.1
(3)
A 		3.7
(-2.1)
A 		3.3
(-2.2)
A 		5.4
(-1.3)
A 		6.3
(-0.9)
A 		14
B 10.8
(2.5)
A 		7.7
(0.8)
A 		11.7
(3)
A 		3.2
(-1.7)
A 		2.2
(-2.3)
A 		0.9
(-3)
A 		11
C 9.9
(-0.1)
A 		12.4
(1.2)
A 		4.7
(-3)
A 		10.8
(0.4)
A 		15.6
(3)
A 		6.5
(-2)
A 		11
avr 7.4
(-0.6) 		11.7
(3) 		6.7
(-1.2) 		5.8
(-2) 		7.7
(-0.4) 		4.6
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185598_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4946
Sequence Type Exemplar sequence
Representative Public ID T10D4.11 NCBI
Target Description T10D4.11 /REP_DB=WormBase Gene ID /WP=CE20051 /TR=Q9TZE7 /GB=AAC68721.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062058(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062058 NCBI Caenorhabditis elegans T10D4.11 (T10D4.11) mRNA, complete cds. 11/11 None
GENEFINDER00000032476 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3143032:3149616:-1 11/11 None
T10D4.11 ENSEMBL cdna:known chromosome:CEL140:II:3148427:3149778:-1 gene:T10D4.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032462 5/11 Cross Hyb Matching Probes None
SNAP00000032463 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3148425-3149777(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T10D4.11
Entrez Gene 188382 Entrez gene
SwissProt Q9TZE7 EMBL-EBI
WormBase CE20051 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68721 Hypothetical protein T10D4.11 [Caenorhabditis elegans] ref|NP_494459.1| T10D4.11 [Caenorhabditis elegans] 1.0E-151
blast AAC68712 Serpentine receptor, class i protein 43 [Caenorhabditis elegans] ref|NP_494458.1| Serpentine Receptor, class I family member (sri-43) [Caenorhabditis elegans] 1.0E-135
blast AAC68720 Serpentine receptor, class i protein 42 [Caenorhabditis elegans] ref|NP_494457.1| Serpentine Receptor, class I family member (sri-42) [Caenorhabditis elegans] 1.0E-103
blast AAC68721 Hypothetical protein T10D4.11 [Caenorhabditis elegans] ref|NP_494459.1| T10D4.11 [Caenorhabditis elegans] 5.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 6.2E-16
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 4.1E-29
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000032476 5 324-346,356-378,399-421,473-495,522-544
Sequence
Target Sequence 
>C. ELEGANS:185598_AT
actacgaggggctaggttgctccagaaccgtatactgcacggtcggctattggacgtggc
taactttgtcctgggagttgtccgacatacccactccaagtgacatttacgaggatacat
ttgtgagttacaagcactttaacacaccctcaagattgattacgtttagtttgctttcac
ttggaatcatacagaccctatgggggtttatgccgggggtcctatcactgacctggtctc
tctgttgggaatgcaattcgtctgaaaatattctgtacacctttaatggaaatcattgca
gtagcaatttcacttgtaatgcagactcccaaacattcatccgatctagttttgacgggt
cggagattcctggtattggtaatcgtacgggtgcaatgctgattcccacgattcccgaag
aagatcagccagaacactcctctataaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTACGAGGGGCTAGGTTGCTCCAG 22 101 149 Antisense
GTTGCTCCAGAACCGTATACTGCAC 532 439 164 Antisense
TACTGCACGGTCGGCTATTGGACGT 125 645 181 Antisense
ACGTGGCTAACTTTGTCCTGGGAGT 184 95 202 Antisense
GTCCTGGGAGTTGTCCGACATACCC 32 471 216 Antisense
CGACATACCCACTCCAAGTGACATT 70 253 231 Antisense
ATTTCACTTGTAATGCAGACTCCCA 92 17 455 Antisense
GACGGGTCGGAGATTCCTGGTATTG 246 375 502 Antisense
GTACGGGTGCAATGCTGATTCCCAC 397 457 533 Antisense
GATTCCCACGATTCCCGAAGAAGAT 497 431 549 Antisense
ATCAGCCAGAACACTCCTCTATAAA 512 27 572 Antisense
Sequence Source Affymetrix Proprietary Database