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[185595_at]

Description T25E12.4B /REP_DB=WormBase Gene ID /WP=CE18283 /TR=O62166 /GB=CAB07477.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_total > 26      4grp_total > 45      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 270.6
(3)
P 		237.3
(1.3)
P 		154.7
(-3)
P 		212.7
(0)
P 		116
B 294.5
(3)
P 		198.1
(-1.3)
P 		206.7
(-0.9)
P 		159.5
(-3)
P 		135
C 236.2
(2.6)
P 		244.1
(3)
P 		193.1
(0.3)
P 		128.9
(-3)
P 		115
avr 267.1
(3) 		226.5
(0.6) 		184.8
(-1.9) 		167
(-3) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 269
1 2 3 4 5 6 range
A 153.1
(0.8)
A 		219.9
(3)
P 		69.5
(-2)
A 		91.7
(-1.2)
A 		38.9
(-3)
A 		125.9
(-0.1)
P 		181
B 124.5
(2.9)
M 		125.8
(3)
A 		91.4
(1.1)
A 		15.6
(-3)
A 		56.9
(-0.8)
A 		76.2
(0.3)
A 		110
C 284.5
(3)
P 		115.1
(-1.6)
A 		86.1
(-2.4)
A 		79.4
(-2.6)
A 		106.5
(-1.8)
A 		63.8
(-3)
A 		221
avr 187.4
(3) 		153.6
(1.4) 		82.3
(-2) 		62.2
(-3) 		67.4
(-2.8) 		88.6
(-1.7) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 98.5
(3)
P 		51.5
(-1.2)
P 		40.4
(-2.2)
P 		31.1
(-3)
P 		67
B 93.9
(3)
P 		48.9
(-1.5)
P 		47.9
(-1.6)
P 		34
(-3)
P 		60
C 78.2
(3)
P 		70.2
(2.2)
P 		67.3
(1.8)
P 		21.7
(-3)
P 		57
avr 90.2
(3) 		56.9
(-0.3) 		51.9
(-0.7) 		28.9
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 28.2
(-1.5)
A 		78.2
(3)
P 		15.1
(-2.7)
A 		23.3
(-2)
A 		11.7
(-3)
A 		30.7
(-1.3)
P 		67
B 43.3
(3)
M 		41.9
(2.8)
A 		22.2
(-0.2)
A 		4.1
(-3)
A 		23.1
(-0.1)
A 		28.4
(0.7)
A 		39
C 105.5
(3)
P 		35.1
(-1.7)
A 		21.9
(-2.6)
A 		16
(-3)
A 		34.5
(-1.8)
A 		21.3
(-2.6)
A 		90
avr 59
(3) 		51.7
(2) 		19.7
(-2.3) 		14.5
(-3) 		23.1
(-1.8) 		26.8
(-1.3) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185595_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14415
Sequence Type Exemplar sequence
Representative Public ID T25E12.4B NCBI
Target Description T25E12.4B /REP_DB=WormBase Gene ID /WP=CE18283 /TR=O62166 /GB=CAB07477.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074839(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074839 NCBI Caenorhabditis elegans T25E12.4b (T25E12.4) mRNA, complete cds. 11/11 A 
SNAP00000025303 ENSEMBL cdna:SNAP chromosome:CEL140:V:16787338:16788109:1 11/11 None
GENEFINDER00000025306 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16787338:16788109:1 11/11 None
T25E12.4b ENSEMBL cdna:known chromosome:CEL140:V:16787329:16788132:1 gene:T25E12.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180121 2 1 NM_074838 194083_s_at (11)
NM_074839 185595_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16787337-16788132(+) 100.0 100.0
Public Domain and Genome References
Ensembl T25E12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07477 Hypothetical protein T25E12.4b [Caenorhabditis elegans] ref|NP_507240.1| T25E12.4b [Caenorhabditis elegans] 5.0E-99
blast CAB07477 Hypothetical protein T25E12.4b [Caenorhabditis elegans] ref|NP_507240.1| T25E12.4b [Caenorhabditis elegans] 3.0E-94
blast CAE70503 Hypothetical protein CBG17106 [Caenorhabditis briggsae] 2.0E-75
blast CAE70503 Hypothetical protein CBG17106 [Caenorhabditis briggsae] 8.0E-75
blast CAB07478 Hypothetical protein T25E12.4a [Caenorhabditis elegans] emb|CAB04830.1| Hypothetical protein T25E12.4a [Caenorhabditis elegans] emb|CAA16519.1| Hypothetical protein T25E12.4a [Caenorhabditis elegans] emb|CAA16430.1| Hypothetical protein T25E12.4a [Caenorhabditis elegans] ref|NP_507239.1| T25E12.4a [Caenorhabditis elegans] 3.0E-20
Sequence
Target Sequence 
>C. ELEGANS:185595_AT
acgatcatcagcgtcggtgactctgacgtcatcaaagactcgagcctcaccgacgaggag
ctctacaacatcattcatgcgtcaccgatcgcacgaaagtcgtcgacagtgtcgtccacc
gactccgggtatctcggctcgtcgggcgcctcgtcgtcgtgtgtgcgaagccgggaagga
tcgacggtgagctcgacgattacggtggaacggacacggcgtggtggatccactgcgtct
tcggaaccggattccgttgctgattcggaaggagctggaagttattcgagcttctcatcg
atagcttcgacagcgtcaaggatgctcggcagagcagcagattgccttgtattaatgaca
aagaggaatggatggagtggagacgccggttcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGATCATCAGCGTCGGTGACTCTG 572 91 124 Antisense
TCATCAAAGACTCGAGCCTCACCGA 269 619 152 Antisense
GACGAGGAGCTCTACAACATCATTC 505 379 175 Antisense
ACATCATTCATGCGTCACCGATCGC 243 105 191 Antisense
AAAGTCGTCGACAGTGTCGTCCACC 577 121 219 Antisense
GACGGTGAGCTCGACGATTACGGTG 307 377 306 Antisense
CGGACACGGCGTGGTGGATCCACTG 595 241 334 Antisense
GAACCGGATTCCGTTGCTGATTCGG 169 345 367 Antisense
CGAGCTTCTCATCGATAGCTTCGAC 241 251 410 Antisense
GACAGCGTCAAGGATGCTCGGCAGA 489 367 432 Antisense
GGATGGAGTGGAGACGCCGGTTCAC 495 513 493 Antisense
Sequence Source Affymetrix Proprietary Database