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[185590_s_at]

Description C45H4.14 /REP_DB=WormBase Gene ID /WP=CE08747 /TR=O44698 /GB=AAC25878.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 78      4grp_total > 0      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 41
(-0.2)
A 		4.8
(-3)
A 		83.2
(3)
A 		80.2
(2.8)
A 		78
B 48.5
(0.3)
P 		78.5
(3)
A 		30.8
(-1.2)
A 		10.9
(-3)
A 		68
C 41.1
(-1.4)
A 		14.8
(-3)
A 		51.1
(-0.8)
A 		113.5
(3)
P 		99
avr 43.5
(-1.2) 		32.7
(-3) 		55
(0.8) 		68.2
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 12.1
(-3)
A 		30.6
(-2.5)
A 		57.7
(-1.7)
A 		36.2
(-2.3)
A 		65
(-1.5)
P 		224.9
(3)
P 		213
B 41.3
(-1.7)
A 		45.4
(-1.6)
A 		34.2
(-2)
A 		4.5
(-3)
A 		111.7
(0.8)
P 		174.5
(3)
P 		170
C 55.2
(-1.3)
A 		57.6
(-1.2)
A 		62.3
(-1)
A 		9.2
(-3)
A 		64.9
(-0.9)
P 		167
(3)
P 		158
avr 36.2
(-2.3) 		44.5
(-2) 		51.4
(-1.8) 		16.6
(-3) 		80.5
(-0.8) 		188.8
(3) 		172
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 14.9
(1)
A 		1
(-3)
A 		21.7
(3)
A 		11.7
(0.1)
A 		21
B 15.5
(1.6)
P 		19.4
(3)
A 		7.1
(-1.3)
A 		2.3
(-3)
A 		17
C 13.6
(0.8)
A 		4.2
(-3)
A 		17.8
(2.5)
A 		19.1
(3)
P 		15
avr 14.7
(2.3) 		8.2
(-3) 		15.5
(3) 		11
(-0.7) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 2.2
(-3)
A 		10.9
(-2)
A 		12.6
(-1.8)
A 		9.2
(-2.2)
A 		19.6
(-1)
P 		54.7
(3)
P 		53
B 14.4
(-1.8)
A 		15.1
(-1.7)
A 		8.3
(-2.3)
A 		1.2
(-3)
A 		45.4
(1.2)
P 		65.1
(3)
P 		64
C 20.5
(-0.9)
A 		17.6
(-1.2)
A 		15.8
(-1.4)
A 		1.8
(-3)
A 		21
(-0.9)
P 		55.9
(3)
P 		54
avr 12.4
(-2.1) 		14.5
(-1.9) 		12.2
(-2.1) 		4.1
(-3) 		28.7
(-0.3) 		58.6
(3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185590_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15356
Sequence Type Exemplar sequence
Representative Public ID C45H4.14 NCBI
Target Description C45H4.14 /REP_DB=WormBase Gene ID /WP=CE08747 /TR=O44698 /GB=AAC25878.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071185(11), NM_182368(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071185 NCBI Caenorhabditis elegans C45H4.14a (C45H4.14) mRNA, complete cds. 11/11 None
NM_182368 NCBI Caenorhabditis elegans C45H4.14b (C45H4.14) mRNA, complete cds. 10/11 None
SNAP00000009344 ENSEMBL cdna:SNAP chromosome:CEL140:V:2158628:2160808:-1 10/11 None
GENEFINDER00000009354 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2158628:2163663:-1 11/11 None
C45H4.14a ENSEMBL cdna:known chromosome:CEL140:V:2158502:2159246:-1 gene:C45H4.14 11/11 None
C45H4.14b ENSEMBL cdna:known chromosome:CEL140:V:2158628:2159335:-1 gene:C45H4.14 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2158627-2159240(-) 71.14 100.0
Public Domain and Genome References
Ensembl C45H4.14
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC25884 Seven tm receptor protein 255 [Caenorhabditis elegans] ref|NP_503587.2| Seven TM Receptor family member (str-255) [Caenorhabditis elegans] 4.0E-88
blast CAE67621 Hypothetical protein CBG13173 [Caenorhabditis briggsae] 1.0E-56
blast CAB04942 Hypothetical protein W09D6.2 [Caenorhabditis elegans] ref|NP_499425.1| Seven TM Receptor family member (str-261) [Caenorhabditis elegans] 2.0E-56
blast AAC25878 Hypothetical protein C45H4.14a [Caenorhabditis elegans] ref|NP_503586.1| C45H4.14a [Caenorhabditis elegans] 3.0E-45
blast AAC25878 Hypothetical protein C45H4.14a [Caenorhabditis elegans] ref|NP_503586.1| C45H4.14a [Caenorhabditis elegans] 4.0E-33
blast AAN84838 Hypothetical protein C45H4.14b [Caenorhabditis elegans] ref|NP_872168.1| C45H4.14b [Caenorhabditis elegans] 1.0E-18
blast AAC25878 Hypothetical protein C45H4.14a [Caenorhabditis elegans] ref|NP_503586.1| C45H4.14a [Caenorhabditis elegans] 1.0E-18
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000009354 7 7-26,41-58,87-109,129-151,163-185,245-263,300-319
Sequence
Target Sequence 
>C. ELEGANS:185590_S_AT
atggccgcgttcaacgcaaagtatctaatcgaccttttgaagagaattcgaagtggagaa
gaaagcatcgattctccggacgcgctggaactgctctacatcccgctgccacttgctcat
gagctcggtgttatcaactcaatggacgggcttgatccatacgatgaaatggagatttcg
tgcaagtcgatcttgaaaaagcttgatgaaaagaagaagactgaggagaagcacagagct
aagcaggagagacaggctcagaagaagggagccaaggagcaagcgaagaaggagaaggcc
gagctaaagaaggaagccaaggagctgcttaaaaaggaaaaagccgaggagaggaagaag
gaatccttgaaaagaaaattgtcggaaaaatcggagccagctgccaagaaggcgaaggct
tctgaggatcaggagaatcgaggagccaccaagaagaagatcacaccaccgaatagaaag
cacaccgcacttttcaaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCCGCGTTCAACGCAAAGTATC 446 53 13 Antisense
GCCACTTGCTCATGAGCTCGGTGTT 313 279 120 Antisense
GGTGTTATCAACTCAATGGACGGGC 170 499 139 Antisense
GGACGGGCTTGATCCATACGATGAA 196 523 156 Antisense
GGAGATTTCGTGCAAGTCGATCTTG 230 519 183 Antisense
GAAGGGAGCCAAGGAGCAAGCGAAG 146 335 276 Antisense
GTCGGAAAAATCGGAGCCAGCTGCC 266 471 393 Antisense
GAAGGCGAAGGCTTCTGAGGATCAG 293 333 420 Antisense
GGAGAATCGAGGAGCCACCAAGAAG 390 519 444 Antisense
GAAGAAGATCACACCACCGAATAGA 216 339 465 Antisense
GAAAGCACACCGCACTTTTCAAAAA 398 359 488 Antisense
Sequence Source Affymetrix Proprietary Database