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[185589_at]

Description Y49F6A.1 /REP_DB=WormBase Gene ID /WP=CE22246 /TR=Q9N3L7 /GB=AAF60724.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 04      4grp_total > 28      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 23.9
(-2.1)
A 		37.5
(3)
A 		21.5
(-3)
A 		23.9
(-2.1)
A 		16
B 34
(-3)
A 		66.2
(3)
A 		37.4
(-2.4)
A 		59.1
(1.7)
A 		32
C 47.6
(1.9)
A 		37
(0.6)
A 		8.2
(-3)
A 		56
(3)
A 		48
avr 35.2
(0.1) 		46.9
(3) 		22.4
(-3) 		46.3
(2.9) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 53
(2.1)
A 		19.4
(-3)
A 		59.2
(3)
A 		37
(-0.3)
A 		32.2
(-1.1)
A 		23.3
(-2.4)
A 		40
B 33.2
(3)
A 		21.1
(-0.2)
A 		19.9
(-0.5)
A 		22.8
(0.3)
A 		23
(0.3)
A 		10.3
(-3)
A 		23
C 13.1
(-2.6)
A 		45.5
(3)
A 		10.9
(-3)
A 		33
(0.8)
A 		33.7
(1)
A 		35.1
(1.2)
A 		35
avr 33.1
(3) 		28.7
(0.4) 		30
(1.2) 		30.9
(1.7) 		29.6
(0.9) 		22.9
(-3) 		10
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 8.7
(3)
A 		8.1
(2.3)
A 		5.6
(-0.6)
A 		3.5
(-3)
A 		5
B 10.8
(-1.4)
A 		16.4
(3)
A 		8.7
(-3)
A 		12.6
(0)
A 		8
C 15.8
(3)
A 		10.7
(0.6)
A 		2.9
(-3)
A 		9.4
(0)
A 		13
avr 11.8
(3) 		11.7
(2.9) 		5.7
(-3) 		8.5
(-0.2) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 9.8
(0.4)
A 		6.9
(-2)
A 		12.9
(3)
A 		9.4
(0.1)
A 		9.7
(0.3)
A 		5.7
(-3)
A 		7
B 11.5
(3)
A 		7
(-0.5)
A 		4.8
(-2.2)
A 		6
(-1.3)
A 		9.3
(1.3)
A 		3.8
(-3)
A 		8
C 4.9
(-1.9)
A 		13.9
(3)
A 		2.8
(-3)
A 		6.6
(-0.9)
A 		10.9
(1.4)
A 		11.8
(1.9)
A 		11
avr 8.7
(0.6) 		9.3
(1.7) 		6.8
(-3) 		7.3
(-2.1) 		10
(3) 		7.1
(-2.4) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185589_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5153
Sequence Type Exemplar sequence
Representative Public ID Y49F6A.1 NCBI
Target Description Y49F6A.1 /REP_DB=WormBase Gene ID /WP=CE22246 /TR=Q9N3L7 /GB=AAF60724.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062192(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062192 NCBI Caenorhabditis elegans Y49F6A.1 (Y49F6A.1) mRNA, complete cds. 11/11 None
SNAP00000032336 ENSEMBL cdna:SNAP chromosome:CEL140:II:3603381:3607378:1 11/11 None
GENEFINDER00000032341 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3603381:3607378:1 11/11 None
Y49F6A.1 ENSEMBL cdna:known chromosome:CEL140:II:3603381:3607378:1 gene:Y49F6A.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3603379-3607377(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y49F6A.1
Entrez Gene 190075 Entrez gene
SwissProt Q9N3L7 EMBL-EBI
WormBase CE22246 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60724 Hypothetical protein Y49F6A.1 [Caenorhabditis elegans] ref|NP_494593.1| Y49F6A.1 [Caenorhabditis elegans] 0.0
blast CAE73814 Hypothetical protein CBG21364 [Caenorhabditis briggsae] 0.0
blast CAA86741 Hypothetical protein C16C10.3 [Caenorhabditis elegans] ref|NP_497834.1| C16C10.3 [Caenorhabditis elegans] sp|Q09249|YQ53_CAEEL Hypothetical protein C16C10.3 in chromosome III 1.0E-173
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 8.4E-24
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 7.7E-95
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 8.4E-24
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 7.7E-95
Sequence
Target Sequence 
>C. ELEGANS:185589_AT
cggcgagggagacttcgatcgggtccaacaggagattacagaaatcagaagctcattcgc
cgaggttcaagaaggctacaagccaagtctagtggtaatcgttgccaacaagacgtccca
tttgagagtgttcccggtggagattcgagaaggtgatacggctatgaagcagaacgtcag
atcgggcacttgcctggatgggaaaatcacgtcggcggggcgggaagagtttgtgctgat
ttctcagacagctttgattagcacagctaagccaacaaagtacaccatcgccgccaatga
tcccaaatggtcgaaaaatgaggttatgaatctcacctatatcctctcgttcgctcatca
ggtctgctaccaaccacctgcagttccacacatcctatatgcagctgataatttggcaaa
gcggggaagaaataatttcatccagcacaaaaaactcggagagctgtccaaaactattca
agccactcttacaaaatactccgatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGCGAGGGAGACTTCGATCGGGTC 617 239 2304 Antisense
TCAGAAGCTCATTCGCCGAGGTTCA 657 623 2348 Antisense
AATCGTTGCCAACAAGACGTCCCAT 333 143 2400 Antisense
AGACGTCCCATTTGAGAGTGTTCCC 279 71 2414 Antisense
GAGTGTTCCCGGTGGAGATTCGAGA 454 379 2429 Antisense
GTCAGATCGGGCACTTGCCTGGATG 194 467 2479 Antisense
GATGGGAAAATCACGTCGGCGGGGC 673 405 2500 Antisense
CGCCGCCAATGATCCCAAATGGTCG 332 257 2592 Antisense
TTATGAATCTCACCTATATCCTCTC 511 681 2627 Antisense
AGTTCCACACATCCTATATGCAGCT 232 65 2685 Antisense
GCCACTCTTACAAAATACTCCGATT 657 277 2785 Antisense
Sequence Source Affymetrix Proprietary Database