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[185586_at]

Description K10F12.2 /REP_DB=WormBase Gene ID /WP=CE12088 /TR=O17233 /GB=AAB71001.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 16      4grp_highest_8_8 > 52      4grp_high_8_8 > 25      6grp_highest_nonorm > 70      6grp_total > 16      4grp_total > 39      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 394
1 2 3 4 range
A 493.2
(3)
P 		220.4
(-1.9)
P 		161.6
(-3)
P 		295.9
(-0.6)
P 		332
B 447.4
(3)
P 		261.5
(-1.7)
P 		211.5
(-3)
P 		297.5
(-0.8)
P 		236
C 555.1
(3)
P 		200.4
(-3)
P 		205.1
(-2.9)
P 		254.4
(-2.1)
P 		355
avr 498.6
(3) 		227.4
(-2.3) 		192.7
(-3) 		282.6
(-1.2) 		306

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 398
1 2 3 4 5 6 range
A 398.2
(3)
P 		376.1
(2.5)
P 		148.9
(-3)
P 		186.6
(-2.1)
P 		170.1
(-2.5)
P 		206.2
(-1.6)
P 		249
B 408.5
(3)
P 		312.4
(0.9)
P 		187.8
(-1.8)
P 		138.5
(-2.9)
P 		163.3
(-2.3)
P 		132.6
(-3)
P 		276
C 510.1
(3)
P 		262.7
(-0.7)
P 		223.3
(-1.3)
P 		145.3
(-2.5)
P 		164.8
(-2.2)
P 		112
(-3)
P 		398
avr 438.9
(3) 		317.1
(0.5) 		186.7
(-2.2) 		156.8
(-2.9) 		166.1
(-2.7) 		150.3
(-3) 		289
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 141
1 2 3 4 range
A 179.6
(3)
P 		47.9
(-2.8)
P 		42.3
(-3)
P 		43.3
(-3)
P 		137
B 142.7
(3)
P 		64.6
(-2)
P 		49
(-3)
P 		63.4
(-2.1)
P 		94
C 183.7
(3)
P 		57.6
(-2.4)
P 		71.5
(-1.8)
P 		42.9
(-3)
P 		141
avr 168.7
(3) 		56.7
(-2.7) 		54.3
(-2.8) 		49.9
(-3) 		119

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 73.4
(-0.6)
P 		133.8
(3)
P 		32.4
(-3)
P 		47.3
(-2.1)
P 		51.3
(-1.9)
P 		50.2
(-1.9)
P 		101
B 142.1
(3)
P 		104
(0.8)
P 		45.6
(-2.5)
P 		36.7
(-3)
P 		66.4
(-1.3)
P 		49.5
(-2.3)
P 		105
C 189.1
(3)
P 		80.2
(-1.1)
P 		56.8
(-2)
P 		29.2
(-3)
P 		53.3
(-2.1)
P 		37.5
(-2.7)
P 		160
avr 134.9
(3) 		106
(1.2) 		44.9
(-2.6) 		37.7
(-3) 		57
(-1.8) 		45.7
(-2.5) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185586_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8154
Sequence Type Exemplar sequence
Representative Public ID K10F12.2 NCBI
Target Description K10F12.2 /REP_DB=WormBase Gene ID /WP=CE12088 /TR=O17233 /GB=AAB71001.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171059(9), NM_171060(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171059 NCBI Caenorhabditis elegans PhosphoLipase C Like family member (pll-1) (pll-1) mRNA, complete cds. 9/11 A 
NM_171060 NCBI Caenorhabditis elegans PhosphoLipase C Like family member (pll-1) (pll-1) mRNA, complete cds. 9/11 A 
GENEFINDER00000031885 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:376432:377457:1 11/11 None
K10F12.3b ENSEMBL cdna:known chromosome:CEL140:III:365948:377550:1 gene:K10F12.3 9/11 A 
K10F12.3a ENSEMBL cdna:known chromosome:CEL140:III:367325:377457:1 gene:K10F12.3 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000031877 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:376431-377457(+) 100.0 100.0
Public Domain and Genome References
Ensembl K10F12.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN72423 Phospholipase c like protein 1, isoform b [Caenorhabditis elegans] ref|NP_741067.1| PhosphoLipase C Like family member (pll-1) [Caenorhabditis elegans] 0.0
blast AAN72422 Phospholipase c like protein 1, isoform a [Caenorhabditis elegans] ref|NP_741068.1| PhosphoLipase C Like family member (pll-1) [Caenorhabditis elegans] 0.0
blast T32374 hypothetical protein K10F12.3 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 1.1E-23
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 2.1E-33
Pfam IPR000008 EMBL-EBI C2 domain 5.5E-27
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 1.1E-23
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 2.1E-33
Pfam IPR000008 EMBL-EBI C2 domain 5.5E-27
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741068.1 1 13-30
K10F12.3a 1 13-30
Sequence
Target Sequence 
>C. ELEGANS:185586_AT
atgcctaccttacagtatccggcgattgtcacaatgggaacacttccggatcaacttcag
cgatcattcaacaagttgaagaatctcattgcacactgtgttacaacactttctcagtcg
gatcagttgctttcaaagatcgaggactcaattcgtcgaatttcggaatgccacgaagaa
ctatcaaaattatgtgcggaggcggggcttaagggacagaaagcaacacgtgccgcagag
aatttcacgtggaatttgagattattaaaagctcagctgaatttgatgaataaatcgcag
gaagaggcgcaggatgtcgttacccaggtattcgacacgggcggtgtgctcggagtacta
tctcaaaagctgatcattcggaaaaatggccggagattttcacgtgtcatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCCTACCTTACAGTATCCGGCGA 643 1 13 Antisense
CAGTATCCGGCGATTGTCACAATGG 369 207 25 Antisense
ATGGGAACACTTCCGGATCAACTTC 289 49 46 Antisense
GAAGAATCTCATTGCACACTGTGTT 291 341 90 Antisense
TTCTCAGTCGGATCAGTTGCTTTCA 172 691 123 Antisense
TGTGCGGAGGCGGGGCTTAAGGGAC 426 541 205 Antisense
GACAGAAAGCAACACGTGCCGCAGA 703 367 227 Antisense
AAGAGGCGCAGGATGTCGTTACCCA 20 155 314 Antisense
GTTACCCAGGTATTCGACACGGGCG 159 443 331 Antisense
GCGGTGTGCTCGGAGTACTATCTCA 527 291 353 Antisense
TGGCCGGAGATTTTCACGTGTCATC 223 545 399 Antisense
Sequence Source Affymetrix Proprietary Database