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[185583_at]

Description H06H21.8 /REP_DB=WormBase Gene ID /WP=CE17966 /TR=Q9TXV0 /GB=AAK29842.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 89      4grp_highest_8_8 > 13      4grp_high_8_8 > 23      6grp_total > 99      4grp_total > 24      6grp_high1_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 313
1 2 3 4 range
A 277.5
(-3)
P 		366.7
(-1.3)
P 		449.1
(0.3)
P 		587.2
(3)
P 		310
B 307.9
(-3)
P 		478.2
(0.6)
P 		465.7
(0.3)
P 		590.7
(3)
P 		283
C 319.8
(-3)
P 		363.8
(-2)
P 		516.9
(1.5)
P 		583.4
(3)
P 		264
avr 301.7
(-3) 		402.9
(-0.9) 		477.2
(0.7) 		587.1
(3) 		285

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 375
1 2 3 4 5 6 range
A 136.7
(-2.2)
P 		188.3
(-1.1)
P 		108.6
(-2.8)
P 		101.3
(-3)
A 		377.5
(3)
P 		303.9
(1.4)
P 		276
B 180.9
(-1.4)
P 		166.9
(-1.6)
P 		78.3
(-3)
A 		141.1
(-2)
P 		453.5
(3)
P 		371.9
(1.7)
P 		375
C 215
(-0.6)
P 		195.5
(-1)
P 		117.3
(-2.8)
P 		109.2
(-3)
P 		301.6
(1.4)
P 		374.5
(3)
P 		265
avr 177.5
(-1.3) 		183.6
(-1.2) 		101.4
(-3) 		117.2
(-2.7) 		377.5
(3) 		350.1
(2.4) 		276
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 101
(0.4)
P 		79.6
(-3)
P 		117.4
(3)
P 		86
(-2)
P 		38
B 98.2
(-3)
P 		118.1
(1.3)
P 		107.9
(-0.9)
P 		125.9
(3)
P 		28
C 105.9
(-2.4)
P 		104.6
(-2.5)
P 		180.2
(3)
P 		98.4
(-3)
P 		82
avr 101.7
(-2.8) 		100.8
(-3) 		135.2
(3) 		103.4
(-2.5) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 165
1 2 3 4 5 6 range
A 25.2
(-2.9)
P 		67
(-0.1)
P 		23.6
(-3)
P 		25.7
(-2.9)
A 		113.9
(3)
P 		74
(0.3)
P 		90
B 63
(-1.4)
P 		55.5
(-1.7)
P 		19
(-3)
A 		37.4
(-2.3)
P 		184.3
(3)
P 		138.7
(1.3)
P 		165
C 79.7
(0.3)
P 		59.7
(-0.8)
P 		29.9
(-2.5)
P 		22
(-3)
P 		97.6
(1.4)
P 		125.4
(3)
P 		103
avr 56
(-1.2) 		60.7
(-1) 		24.2
(-3) 		28.4
(-2.8) 		131.9
(3) 		112.7
(1.9) 		108
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185583_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10387
Sequence Type Exemplar sequence
Representative Public ID H06H21.8 NCBI
Target Description H06H21.8 /REP_DB=WormBase Gene ID /WP=CE17966 /TR=Q9TXV0 /GB=AAK29842.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028083(9), NM_001028084(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028083 NCBI Caenorhabditis elegans H06H21.8a (H06H21.8) mRNA, complete cds. 9/11 None
NM_001028084 NCBI Caenorhabditis elegans H06H21.8b (H06H21.8) mRNA, complete cds. 11/11 None
SNAP00000018257 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4817512:4821064:-1 11/11 None
GENEFINDER00000018265 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4817512:4821064:-1 11/11 None
H06H21.8a ENSEMBL cdna:known chromosome:CEL140:IV:4817378:4821064:-1 gene:H06H21.8 9/11 None
H06H21.8b ENSEMBL cdna:known chromosome:CEL140:IV:4817512:4821064:-1 gene:H06H21.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4817517-4821070(-) 100.0 100.0
Public Domain and Genome References
Ensembl H06H21.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29842 Hypothetical protein H06H21.8a [Caenorhabditis elegans] ref|NP_001023254.1| H06H21.8a [Caenorhabditis elegans] 0.0
blast AAQ01531 Hypothetical protein H06H21.8b [Caenorhabditis elegans] ref|NP_001023255.1| H06H21.8b [Caenorhabditis elegans] pir||G88678 protein H06H21.8 [imported] - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004119 EMBL-EBI Protein of unknown function DUF227 1.3E-7
Sequence
Target Sequence 
>C. ELEGANS:185583_AT
gcacatggtagagagactttttccgaaggaatgcagaatatgatgtttgaagaagcattg
acattggaaaatgtctcaccggaagtatttggaaatgacagaattcgaaatatcaaatgg
tccttcgactattctatcaagaacaaagcaactgcggacgctatttctgcttttcctgga
ataatctgccatgctgatctgaacgtgacaaatgtgctatggaagaaggatagtgcaaaa
gatgaaatttcagcaattattgattatcaaatgttatttattggatcaattgcatttgat
attattcgagtacttactttgggattgaacagagaaattcgaagaaagatgactcaaaac
tatttggatcactatcataaaactctcacagagctctctaatggaaaagctccgttttct
atggaagagctccttcaccaatactccctgatctatccattttcatcaaactttagcttg
tttggaatcgctttgtacatcaaaatgtattctgatgggacacttggaaacccagaggac
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCACATGGTAGAGAGACTTTTTCCG 545 313 556 Antisense
CATTGGAAAATGTCTCACCGGAAGT 266 217 617 Antisense
ATGGTCCTTCGACTATTCTATCAAG 430 49 672 Antisense
GCAACTGCGGACGCTATTTCTGCTT 5 321 703 Antisense
GCTATTTCTGCTTTTCCTGGAATAA 580 303 715 Antisense
TCTGCCATGCTGATCTGAACGTGAC 432 609 740 Antisense
GGATCACTATCATAAAACTCTCACA 296 511 921 Antisense
TGGAAGAGCTCCTTCACCAATACTC 245 547 977 Antisense
CTCCCTGATCTATCCATTTTCATCA 591 233 999 Antisense
GTTTGGAATCGCTTTGTACATCAAA 11 453 1035 Antisense
GGGACACTTGGAAACCCAGAGGACA 423 493 1072 Antisense
Sequence Source Affymetrix Proprietary Database