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[185578_at]

Description C45H4.13 /REP_DB=WormBase Gene ID /WP=CE08746 /TR=O44693 /GB=AAC25880.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 77      4grp_total > 0      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 54.6
(-2.5)
A 		51.3
(-3)
A 		76.9
(0.9)
A 		90.3
(3)
P 		39
B 55.1
(-0.3)
A 		21.8
(-3)
A 		56.9
(-0.1)
A 		94.9
(3)
P 		73
C 63
(0.5)
A 		33.9
(-3)
A 		46.6
(-1.5)
A 		83.2
(3)
P 		49
avr 57.6
(-0.6) 		35.7
(-3) 		60.1
(-0.3) 		89.5
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 330
1 2 3 4 5 6 range
A 39.1
(-3)
A 		46.6
(-2.9)
A 		64.2
(-2.5)
A 		71.7
(-2.4)
A 		141.9
(-1.1)
P 		368.5
(3)
P 		329
B 59.2
(-3)
A 		74.7
(-2.7)
A 		68.5
(-2.8)
A 		108.4
(-2)
P 		93.1
(-2.3)
P 		369.3
(3)
P 		310
C 55.8
(-3)
A 		62.7
(-2.8)
A 		55
(-3)
A 		87.9
(-2.2)
A 		96.2
(-2)
P 		298.7
(3)
P 		244
avr 51.4
(-3) 		61.3
(-2.8) 		62.6
(-2.8) 		89.3
(-2.2) 		110.4
(-1.8) 		345.5
(3) 		294
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 19.9
(2.9)
A 		11.1
(-3)
A 		20.1
(3)
A 		13.2
(-1.6)
P 		9
B 17.6
(1.9)
A 		5.4
(-3)
A 		13.2
(0.2)
A 		20.2
(3)
P 		15
C 20.9
(3)
A 		9.8
(-3)
A 		16.3
(0.5)
A 		14
(-0.7)
P 		11
avr 19.5
(3) 		8.8
(-3) 		16.5
(1.3) 		15.8
(0.9) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 7.2
(-3)
A 		16.6
(-2.3)
A 		14
(-2.5)
A 		18.2
(-2.2)
A 		42.8
(-0.4)
P 		89.7
(3)
P 		83
B 20.6
(-2.8)
A 		24.9
(-2.6)
A 		16.6
(-3)
A 		28.7
(-2.4)
P 		37.8
(-1.9)
P 		137.7
(3)
P 		121
C 20.7
(-2.5)
A 		19.1
(-2.6)
A 		14
(-3)
A 		17.7
(-2.7)
A 		31.1
(-1.8)
P 		100
(3)
P 		86
avr 16.2
(-2.9) 		20.2
(-2.7) 		14.9
(-3) 		21.5
(-2.6) 		37.2
(-1.6) 		109.1
(3) 		94
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185578_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15735
Sequence Type Exemplar sequence
Representative Public ID C45H4.13 NCBI
Target Description C45H4.13 /REP_DB=WormBase Gene ID /WP=CE08746 /TR=O44693 /GB=AAC25880.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071180(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071180 NCBI Caenorhabditis elegans C45H4.13 (C45H4.13) mRNA, complete cds. 10/11 None
SNAP00000009339 ENSEMBL cdna:SNAP chromosome:CEL140:V:2148131:2149360:-1 10/11 None
GENEFINDER00000009361 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2148131:2150544:-1 11/11 None
C45H4.13 ENSEMBL cdna:known chromosome:CEL140:V:2148485:2150598:-1 gene:C45H4.13 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2148441-2150598(-) 97.92 100.0
Public Domain and Genome References
Gene Title
Ensembl C45H4.13
Entrez Gene 183494 Entrez gene
SwissProt O44693 EMBL-EBI
WormBase CE35961 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC25880 Hypothetical protein C45H4.13 [Caenorhabditis elegans] ref|NP_503581.2| C45H4.13 [Caenorhabditis elegans] 5.0E-86
blast AAC25880 Hypothetical protein C45H4.13 [Caenorhabditis elegans] ref|NP_503581.2| C45H4.13 [Caenorhabditis elegans] 7.0E-64
blast AAC25880 Hypothetical protein C45H4.13 [Caenorhabditis elegans] ref|NP_503581.2| C45H4.13 [Caenorhabditis elegans] 7.0E-9
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503581.2 3 115-137,152-174,181-203
C45H4.13 3 115-137,152-174,181-203
SNAP00000009339 1 173-195
GENEFINDER00000009361 4 97-119,134-156,163-185,278-300
Sequence
Target Sequence 
>C. ELEGANS:185578_AT
gagagaattcgacacgctcgtgacagtagcggctcacacgaatcctactctggctccagc
tccagtagctcctatagctcgagcgatagcagtagctactcttcgtacactccaagcatc
tcatcaaacagtgactacacttcatacagccccagttactatagctactcttcatatagc
ccgagctactcctcctacggttccagctactctccaagctactacagcaactattatgga
gg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAGAATTCGACACGCTCGTGACA 283 395 639 Antisense
GACACGCTCGTGACAGTAGCGGCTC 670 367 649 Antisense
AGTAGCGGCTCACACGAATCCTACT 348 63 663 Antisense
CTCCAGTAGCTCCTATAGCTCGAGC 651 235 698 Antisense
TAGCAGTAGCTACTCTTCGTACACT 248 651 725 Antisense
TACACTCCAAGCATCTCATCAAACA 463 643 744 Antisense
ACAGTGACTACACTTCATACAGCCC 344 107 766 Antisense
AGCCCCAGTTACTATAGCTACTCTT 271 83 786 Antisense
AGCTACTCTTCATATAGCCCGAGCT 586 79 801 Antisense
CAGCTACTCTCCAAGCTACTACAGC 676 203 842 Antisense
GCTACTACAGCAACTATTATGGAGG 231 303 856 Antisense
Sequence Source Affymetrix Proprietary Database