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[185573_at]

Description T08A9.10 /REP_DB=WormBase Gene ID /WP=CE04925 /TR=Q22337 /GB=AAA81415.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 52      4grp_total > 37      6grp_cgc > 90      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 46.1
(2.7)
A 		48.2
(3)
A 		5.1
(-3)
A 		5.8
(-2.9)
A 		43
B 12.5
(3)
A 		4
(-1.8)
A 		4.1
(-1.8)
A 		1.9
(-3)
A 		11
C 21.2
(-0.7)
A 		9.6
(-2.3)
A 		4.8
(-3)
A 		47.7
(3)
A 		43
avr 26.6
(3) 		20.6
(1.4) 		4.7
(-3) 		18.5
(0.8) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 37.9
(-0.4)
A 		23.6
(-1.5)
A 		82.8
(3)
A 		51.2
(0.6)
A 		22.8
(-1.5)
A 		3.1
(-3)
A 		80
B 25.9
(-1.1)
A 		30.3
(-0.3)
A 		39.3
(1.3)
A 		37.3
(0.9)
A 		49
(3)
A 		15.4
(-3)
A 		34
C 31.7
(-0.3)
A 		28.1
(-1)
A 		47.5
(2.8)
A 		21
(-2.5)
A 		48.3
(3)
P 		18.3
(-3)
A 		30
avr 31.8
(-0.4) 		27.3
(-1) 		56.5
(3) 		36.5
(0.3) 		40
(0.8) 		12.3
(-3) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 16.8
(3)
A 		10.5
(0.6)
A 		1.3
(-2.8)
A 		0.8
(-3)
A 		16
B 4
(3)
A 		1
(-2)
A 		0.9
(-2.2)
A 		0.4
(-3)
A 		4
C 7
(2)
A 		2.8
(-2)
A 		1.7
(-3)
A 		8
(3)
A 		6
avr 9.3
(3) 		4.8
(-0.4) 		1.3
(-3) 		3.1
(-1.7) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 7
(-0.8)
A 		8.4
(-0.3)
A 		18
(3)
A 		13
(1.3)
A 		6.9
(-0.8)
A 		0.7
(-3)
A 		17
B 9
(-1.6)
A 		10.1
(-1.1)
A 		9.5
(-1.4)
A 		9.9
(-1.2)
A 		19.9
(3)
A 		5.7
(-3)
A 		14
C 11.8
(1)
A 		8.6
(-0.7)
A 		12.1
(1.2)
A 		4.2
(-3)
A 		15.6
(3)
P 		6.1
(-2)
A 		11
avr 9.3
(0.1) 		9
(-0.1) 		13.2
(2.5) 		9
(-0.1) 		14.1
(3) 		4.2
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185573_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16511
Sequence Type Exemplar sequence
Representative Public ID T08A9.10 NCBI
Target Description T08A9.10 /REP_DB=WormBase Gene ID /WP=CE04925 /TR=Q22337 /GB=AAA81415.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076835(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076835 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-6) (spp-6) mRNA, complete cds. 11/11 None
SNAP00000001753 ENSEMBL cdna:SNAP chromosome:CEL140:X:7317286:7317607:1 11/11 None
GENEFINDER00000001765 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7317334:7317607:1 11/11 None
T08A9.10 ENSEMBL cdna:known chromosome:CEL140:X:7317334:7317607:1 gene:T08A9.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7317332-7317606(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-6
Ensembl T08A9.10
Entrez Gene 188267 Entrez gene
SwissProt Q22337 EMBL-EBI
WormBase CE38545 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_509236 SaPosin-like Protein family member (spp-6) [Caenorhabditis elegans] gb|AAA81415.2| Saposin-like protein family protein 6 [Caenorhabditis elegans] 2.0E-39
blast C89567 protein T08A9.10 [imported] - Caenorhabditis elegans 4.0E-29
blast NP_509236 SaPosin-like Protein family member (spp-6) [Caenorhabditis elegans] gb|AAA81415.2| Saposin-like protein family protein 6 [Caenorhabditis elegans] 4.0E-29
blast NP_509237 SaPosin-like Protein family member (spp-4) [Caenorhabditis elegans] gb|AAA81416.1| Saposin-like protein family protein 4 [Caenorhabditis elegans] 3.0E-24
Protein Domains
Database ID Description E-Value
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 5.7E-11
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 5.7E-11
Sequence
Target Sequence 
>C. ELEGANS:185573_AT
gcaaacttgcggtgaaaagtgccgatggagatgctgacaaagacaccaacgacatcaaaa
aggatttcgatgcaaaatgtaaaaaggcattccactcgattcagtttgcgccacgagagt
gtgagcattacgtcgataaaaagctggatccaatcattaaagagctcgaaagtggaacat
ctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAACTTGCGGTGAAAAGTGCCGA 184 317 17 Antisense
GTGAAAAGTGCCGATGGAGATGCTG 444 479 28 Antisense
GGAGATGCTGACAAAGACACCAACG 88 519 43 Antisense
GACAAAGACACCAACGACATCAAAA 27 367 52 Antisense
GTAAAAAGGCATTCCACTCGATTCA 327 461 95 Antisense
GCATTCCACTCGATTCAGTTTGCGC 326 307 103 Antisense
GATTCAGTTTGCGCCACGAGAGTGT 123 431 114 Antisense
GAGAGTGTGAGCATTACGTCGATAA 597 395 131 Antisense
ACGTCGATAAAAAGCTGGATCCAAT 328 95 146 Antisense
TGGATCCAATCATTAAAGAGCTCGA 439 549 161 Antisense
AAGAGCTCGAAAGTGGAACATCTCC 6 153 176 Antisense
Sequence Source Affymetrix Proprietary Database