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[185556_at]

Description C32E12.1 /REP_DB=WormBase Gene ID /WP=CE04133 /TR=P91122 /GB=AAB53878.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 89      4grp_total > 43      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 29.6
(-1.2)
A 		67.9
(3)
A 		12.7
(-3)
A 		27.5
(-1.4)
A 		55
B 42.9
(0.4)
A 		46
(0.7)
A 		66.3
(3)
A 		12.6
(-3)
A 		54
C 36.9
(-3)
A 		93.3
(3)
A 		39.5
(-2.7)
A 		64.7
(-0)
A 		56
avr 36.5
(-2.7) 		69.1
(3) 		39.5
(-2.2) 		34.9
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 14.9
(-2.4)
A 		9.9
(-2.7)
A 		13.1
(-2.5)
A 		5.8
(-3)
A 		98.1
(3)
A 		68.2
(1.1)
A 		92
B 19.3
(-2.4)
A 		34.6
(-1.5)
A 		9.3
(-3)
A 		50.5
(-0.6)
A 		58.2
(-0.1)
A 		110.7
(3)
A 		101
C 7.4
(-3)
A 		45.8
(-0.8)
A 		10.5
(-2.8)
A 		20.9
(-2.2)
A 		61.2
(0.1)
A 		112.2
(3)
A 		105
avr 13.9
(-2.8) 		30.1
(-1.7) 		11
(-3) 		25.7
(-2) 		72.5
(1.3) 		97
(3) 		86
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 10.8
(0.9)
A 		14.7
(3)
A 		3.3
(-3)
A 		4
(-2.6)
A 		11
B 13.7
(2.2)
A 		11.4
(1.1)
A 		15.4
(3)
A 		2.7
(-3)
A 		13
C 12.2
(-2.5)
A 		26.8
(3)
A 		13.8
(-1.9)
A 		10.9
(-3)
A 		16
avr 12.2
(0.2) 		17.6
(3) 		10.8
(-0.5) 		5.9
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 2.8
(-2.7)
A 		3.5
(-2.6)
A 		2.8
(-2.7)
A 		1.5
(-3)
A 		29.6
(3)
A 		16.6
(0.2)
A 		28
B 6.7
(-2.3)
A 		11.5
(-1.6)
A 		2.3
(-3)
A 		13.4
(-1.3)
A 		23.6
(0.3)
A 		41.3
(3)
A 		39
C 2.8
(-3)
A 		14
(-1.1)
A 		2.7
(-3)
A 		4.2
(-2.7)
A 		19.8
(-0.1)
A 		37.6
(3)
A 		35
avr 4.1
(-2.7) 		9.7
(-1.5) 		2.6
(-3) 		6.4
(-2.2) 		24.3
(1.5) 		31.8
(3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185556_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.514
Sequence Type Exemplar sequence
Representative Public ID C32E12.1 NCBI
Target Description C32E12.1 /REP_DB=WormBase Gene ID /WP=CE04133 /TR=P91122 /GB=AAB53878.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059146(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059146 NCBI Caenorhabditis elegans C32E12.1 (C32E12.1) mRNA, complete cds. 11/11 None
SNAP00000039924 ENSEMBL cdna:SNAP chromosome:CEL140:I:5233064:5235225:1 11/11 None
GENEFINDER00000039929 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5233104:5235225:1 11/11 None
C32E12.1 ENSEMBL cdna:known chromosome:CEL140:I:5233064:5235225:1 gene:C32E12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5233063-5235225(+) 89.63 100.0
Public Domain and Genome References
Gene Title
Ensembl C32E12.1
Entrez Gene 183124 Entrez gene
SwissProt P91122 EMBL-EBI
WormBase CE04133 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB53878 Hypothetical protein C32E12.1 [Caenorhabditis elegans] ref|NP_491547.1| C32E12.1 [Caenorhabditis elegans] 0.0
blast CAE67372 Hypothetical protein CBG12848 [Caenorhabditis briggsae] 2.0E-62
blast CAE67372 Hypothetical protein CBG12848 [Caenorhabditis briggsae] 7.0E-62
Sequence
Target Sequence 
>C. ELEGANS:185556_AT
gtcgttctcaccggttaacatagtctctacagttcctgatcaagtcaagaagccaggcaa
aaattctccgaaggatgaacatgttatgaaaactccaccaattgagaacccagttgccgc
caagaagaacccatttgatccactacgttcagaactggagaccccgattcaagaacctgc
atttgtagtcaatcgtggtgatacaaagatcaagaatatggagatcaagttggtcaactt
ggatttggttggcagaaccgggatgtacatggaaagatgtggaatgaatgctgagagaaa
ggaggcatttgactggcttgcttacaatcgtcacgacttgaacaataaacagaaagagtt
ttcacagaacttgtccgtggctctaagtgatattggaattaccaaagaactcctcatgcg
cacaataatctacctcaaatcgcataatcaaatcacgccatctgaaatggatatggtttt
gaaggacgtgaaaggatgtaaaggagatactgtcagtttctcgcaactttccgtcttcct
tcagtcacattgtgcagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGTTCTCACCGGTTAACATAGTC 11 473 1080 Antisense
AACATAGTCTCTACAGTTCCTGATC 166 139 1096 Antisense
ACCAATTGAGAACCCAGTTGCCGCC 434 83 1176 Antisense
AGAAGAACCCATTTGATCCACTACG 298 73 1202 Antisense
TCAGAACTGGAGACCCCGATTCAAG 484 621 1228 Antisense
GACTGGCTTGCTTACAATCGTCACG 598 375 1390 Antisense
GAACTTGTCCGTGGCTCTAAGTGAT 123 351 1446 Antisense
TTACCAAAGAACTCCTCATGCGCAC 101 667 1478 Antisense
TCATGCGCACAATAATCTACCTCAA 57 621 1493 Antisense
GATACTGTCAGTTTCTCGCAACTTT 14 425 1585 Antisense
TCTTCCTTCAGTCACATTGTGCAGT 593 617 1613 Antisense
Sequence Source Affymetrix Proprietary Database