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[185555_s_at]

Description Y47H10A.1 /REP_DB=WormBase Gene ID /WP=CE24374 /TR=Q9U2B2 /GB=CAB60389.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 34      6grp_cgc > 89      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 19.1
(-3)
A 		19.9
(-2.9)
A 		20.4
(-2.8)
A 		51.5
(3)
A 		32
B 4.6
(-2.8)
A 		17.4
(2.7)
A 		18.1
(3)
A 		4.1
(-3)
A 		14
C 13.9
(-2.3)
A 		39.1
(3)
A 		19.5
(-1.1)
A 		10.5
(-3)
A 		29
avr 12.5
(-3) 		25.5
(3) 		19.3
(0.1) 		22
(1.4) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 13.7
(-1.6)
A 		9.3
(-2.7)
A 		8.8
(-2.8)
A 		7.9
(-3)
A 		25.3
(1.3)
A 		32.1
(3)
A 		24
B 9.1
(-2.6)
A 		5.5
(-3)
A 		10.5
(-2.4)
A 		59.7
(3)
A 		32.2
(-0)
A 		53.7
(2.3)
A 		54
C 19.2
(-2.2)
A 		16.3
(-2.4)
A 		47.2
(-0.6)
A 		5.9
(-3)
A 		107.5
(3)
A 		24.6
(-1.9)
A 		102
avr 14
(-2.5) 		10.4
(-3) 		22.2
(-1.4) 		24.5
(-1.1) 		55
(3) 		36.8
(0.6) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 7
(2.1)
A 		4.3
(-3)
A 		5.3
(-1.1)
A 		7.5
(3)
A 		3
B 1.5
(-1.9)
A 		4.3
(3)
A 		4.2
(2.8)
A 		0.9
(-3)
A 		3
C 4.6
(-1.2)
A 		11.2
(3)
A 		6.8
(0.2)
A 		1.8
(-3)
A 		9
avr 4.4
(-1.1) 		6.6
(3) 		5.4
(0.8) 		3.4
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 2.5
(-2.4)
A 		3.3
(-1.6)
A 		1.9
(-3)
A 		2
(-2.9)
A 		7.6
(2.8)
A 		7.8
(3)
A 		6
B 3.2
(-2.5)
A 		1.8
(-3)
A 		2.5
(-2.8)
A 		15.8
(1.6)
A 		13.1
(0.7)
A 		20
(3)
A 		18
C 7.1
(-1.9)
A 		5
(-2.3)
A 		12
(-1.1)
A 		1.2
(-3)
A 		34.8
(3)
A 		8.2
(-1.8)
A 		34
avr 4.3
(-2.6) 		3.4
(-3) 		5.5
(-2.2) 		6.3
(-1.8) 		18.5
(3) 		12
(0.4) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185555_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1588
Sequence Type Exemplar sequence
Representative Public ID Y47H10A.1 NCBI
Target Description Y47H10A.1 /REP_DB=WormBase Gene ID /WP=CE24374 /TR=Q9U2B2 /GB=CAB60389.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060651(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060651 NCBI Caenorhabditis elegans CaLPain family member (clp-3) (clp-3) mRNA, complete cds. 11/11 None
GENEFINDER00000005134 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12079992:12095952:-1 10/11 None
SNAP00000005123 ENSEMBL cdna:SNAP chromosome:CEL140:I:12082054:12082521:-1 10/11 None
Y47H10A.1 ENSEMBL cdna:known chromosome:CEL140:I:12081265:12090697:-1 gene:Y47H10A.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12081227-12090628(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol clp-3
Ensembl Y47H10A.1
Entrez Gene 173089 Entrez gene
SwissProt Q9U2B2 EMBL-EBI
WormBase CE24374 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
6508 proteolysis inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4198 calpain activity inferred from electronic annotation QuickGO AmiGO
8234 cysteine-type peptidase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60389 Hypothetical protein Y47H10A.1 [Caenorhabditis elegans] ref|NP_493052.1| CaLPain family member (clp-3) [Caenorhabditis elegans] 0.0
blast AAF36079 Calpain family protein 6 [Caenorhabditis elegans] ref|NP_500081.1| CaLPain family member (clp-6) [Caenorhabditis elegans] 0.0
blast CAB60388 Hypothetical protein Y47H10A.2 [Caenorhabditis elegans] ref|NP_493053.2| Y47H10A.2 [Caenorhabditis elegans] 0.0
blast AAF36080 Calpain family protein 7 [Caenorhabditis elegans] ref|NP_500082.1| CaLPain family member (clp-7) [Caenorhabditis elegans] 1.0E-170
blast CAB60389 Hypothetical protein Y47H10A.1 [Caenorhabditis elegans] ref|NP_493052.1| CaLPain family member (clp-3) [Caenorhabditis elegans] 3.0E-89
Protein Domains
Database ID Description E-Value
scop a.1.1.Globins All alpha proteins; Globin-like; Globin-like; Globins 9.30000019073486
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.8E-72
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 7.0E-25
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 8.2E-29
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.8E-72
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 7.0E-25
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 0.0018
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.4E-20
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 8.2E-29
Sequence
Target Sequence 
>C. ELEGANS:185555_S_AT
gtactgctgtaatctgtcggctgatacgatgcatgaagtcgagaagatgacagaggtgaa
ggtcatggagcatcagagccagcaatggatccaagcatcatttgagggagaatggagttc
ccggataggtactgccgggggatgcgatgaccacgacaccttctgcactaatctacagta
tgagatacacttccgcgccaccgactcatacgactccaaccacaaatgcacaataattgc
cgctctttttcaaaaaaatcgtgatcactgtgtgtacaagggtttagaactattcctaat
cggactcctagtgtacgaaatgccgggtcccaatgagaaagtgacccccgagatggtcaa
atcacagacgcccattgcggattccaaacttttcaaagattctcgtgaggctaacatacg
attcactgttccgcttgggcactatgtgattgtaccgagcacttatgatccggaccagga
cggcgagtttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTACTGCTGTAATCTGTCGGCTGAT 187 459 1335 Antisense
GATAGGTACTGCCGGGGGATGCGAT 107 407 1458 Antisense
GACACCTTCTGCACTAATCTACAGT 245 367 1489 Antisense
GCACAATAATTGCCGCTCTTTTTCA 663 315 1562 Antisense
GAACTATTCCTAATCGGACTCCTAG 492 349 1621 Antisense
TAGTGTACGAAATGCCGGGTCCCAA 423 569 1643 Antisense
GACGCCCATTGCGGATTCCAAACTT 686 379 1701 Antisense
AACATACGATTCACTGTTCCGCTTG 492 137 1747 Antisense
TGTTCCGCTTGGGCACTATGTGATT 585 561 1761 Antisense
GTACCGAGCACTTATGATCCGGACC 112 459 1786 Antisense
GATCCGGACCAGGACGGCGAGTTTC 660 413 1801 Antisense
Sequence Source Affymetrix Proprietary Database