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[185543_at]

Description F08D12.4 /REP_DB=WormBase Gene ID /WP=CE09237 /TR=P91236 /GB=AAB37927.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 47      4grp_total > 07      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 29.3
(1)
A 		9.4
(-3)
A 		39.3
(3)
A 		10.9
(-2.7)
A 		30
B 11.3
(0.3)
A 		4.9
(-3)
A 		7.8
(-1.5)
A 		16.5
(3)
A 		12
C 12.9
(-0.6)
A 		8.8
(-2.3)
A 		7.2
(-3)
A 		21.6
(3)
A 		14
avr 17.8
(2.8) 		7.7
(-3) 		18.1
(3) 		16.3
(2) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 20.6
(-0.1)
A 		10.7
(-2.1)
A 		16.4
(-1)
A 		6.5
(-3)
A 		10.7
(-2.1)
A 		35.9
(3)
A 		29
B 36.9
(3)
A 		27.9
(1.2)
A 		6.6
(-3)
A 		14.8
(-1.4)
A 		8.8
(-2.6)
A 		10.2
(-2.3)
A 		30
C 10.4
(-2.2)
A 		17.7
(-0.8)
A 		7.7
(-2.7)
A 		6.2
(-3)
A 		6.4
(-3)
A 		37.1
(3)
A 		31
avr 22.6
(1.4) 		18.8
(0.2) 		10.2
(-2.5) 		9.2
(-2.8) 		8.6
(-3) 		27.7
(3) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 10.7
(3)
A 		2
(-2.7)
A 		10.3
(2.7)
A 		1.6
(-3)
A 		9
B 3.6
(3)
A 		1.2
(-3)
A 		1.8
(-1.5)
A 		3.5
(2.8)
A 		2
C 4.3
(3)
A 		2.5
(-3)
A 		2.5
(-3)
A 		3.6
(0.7)
A 		2
avr 6.2
(3) 		1.9
(-3) 		4.9
(1.2) 		2.9
(-1.6) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 3.8
(-1.2)
A 		3.8
(-1.2)
A 		3.6
(-1.4)
A 		1.7
(-3)
A 		3.2
(-1.7)
A 		8.7
(3)
A 		7
B 12.8
(3)
A 		9.3
(1.1)
A 		1.6
(-3)
A 		3.9
(-1.8)
A 		3.6
(-1.9)
A 		3.8
(-1.8)
A 		11
C 3.8
(-1.6)
A 		5.4
(-0.8)
A 		2
(-2.6)
A 		1.2
(-3)
A 		2.1
(-2.5)
A 		12.4
(3)
A 		11
avr 6.8
(1.5) 		6.2
(0.9) 		2.4
(-2.9) 		2.3
(-3) 		3
(-2.3) 		8.3
(3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185543_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5804
Sequence Type Exemplar sequence
Representative Public ID F08D12.4 NCBI
Target Description F08D12.4 /REP_DB=WormBase Gene ID /WP=CE09237 /TR=P91236 /GB=AAB37927.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061961(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061961 NCBI Caenorhabditis elegans F08D12.4 (F08D12.4) mRNA, complete cds. 11/11 None
SNAP00000023718 ENSEMBL cdna:SNAP chromosome:CEL140:II:2778218:2779331:1 11/11 None
GENEFINDER00000023732 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2776829:2779356:1 11/11 None
F08D12.4 ENSEMBL cdna:known chromosome:CEL140:II:2778218:2779282:1 gene:F08D12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2778216-2779281(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F08D12.4
Entrez Gene 173624 Entrez gene
SwissProt P91236 EMBL-EBI
WormBase CE09237 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37927 Hypothetical protein F08D12.4 [Caenorhabditis elegans] ref|NP_494362.1| F08D12.4 [Caenorhabditis elegans] 1.0E-126
blast AAB37927 Hypothetical protein F08D12.4 [Caenorhabditis elegans] ref|NP_494362.1| F08D12.4 [Caenorhabditis elegans] 1.0E-120
blast AAB37927 Hypothetical protein F08D12.4 [Caenorhabditis elegans] ref|NP_494362.1| F08D12.4 [Caenorhabditis elegans] 1.0E-97
blast AAY86244 Hypothetical protein C50E3.14 [Caenorhabditis elegans] 2.0E-17
blast AAY86244 Hypothetical protein C50E3.14 [Caenorhabditis elegans] 1.0E-15
blast NP_504966 C50E3.2 [Caenorhabditis elegans] gb|AAB04888.1| Hypothetical protein C50E3.2 [Caenorhabditis elegans] 2.0E-14
blast AAY86244 Hypothetical protein C50E3.14 [Caenorhabditis elegans] 1.0E-13
blast NP_504966 C50E3.2 [Caenorhabditis elegans] gb|AAB04888.1| Hypothetical protein C50E3.2 [Caenorhabditis elegans] 2.0E-13
Sequence
Target Sequence 
>C. ELEGANS:185543_AT
gaagagatccgtgccaaacaccaacctgagccaacctatcacctcctgcccagcttctac
tgtgaaatttcccacctgtaaagttgggtggaagcttttcccacgttacaatacttattg
gtgtctcacgctaggcatcaatcgaagggaacgtgacggatacttgctacagtttgatca
aagtttcagttatgttgcttgcagatgggatggatcggagttcatcgatggtagaataag
tgggctggagagcaatgaggagagagcttacgcgacggaccgagcttatcaaataatgaa
gcaaaacatgcgcgagacattgttcgtattcccatcaaatgtgcgactgggctggggtgt
gtgggttgatggagtgaaagatgtggcaggtcaagtcaagtacagtgacaccacgctcaa
cttaaaactcggatacaaatgggatatcgggcagccaaattgggtctcacgaaagcaggc
atgcgttatgttaatggttccgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGAGATCCGTGCCAAACACCAAC 364 339 156 Antisense
TCTACTGTGAAATTTCCCACCTGTA 517 613 211 Antisense
GCTTTTCCCACGTTACAATACTTAT 511 303 249 Antisense
TATTGGTGTCTCACGCTAGGCATCA 168 665 271 Antisense
GACATTGTTCGTATTCCCATCAAAT 48 371 471 Antisense
TGGGGTGTGTGGGTTGATGGAGTGA 646 551 508 Antisense
AAGTACAGTGACACCACGCTCAACT 352 157 553 Antisense
ACCACGCTCAACTTAAAACTCGGAT 447 83 565 Antisense
ATCGGGCAGCCAAATTGGGTCTCAC 604 35 601 Antisense
TGGGTCTCACGAAAGCAGGCATGCG 7 555 616 Antisense
GCATGCGTTATGTTAATGGTTCCGG 705 307 634 Antisense
Sequence Source Affymetrix Proprietary Database