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[185531_at]

Description Y53F4B.20 /REP_DB=WormBase Gene ID /WP=CE25399 /TR=Q9NAA0 /GB=CAB70109.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 16      6grp_total > 16      4grp_total > 52      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 154.7
(3)
P 		148.8
(2.4)
P 		125.9
(0.2)
P 		92.4
(-3)
A 		62
B 154
(3)
P 		151.2
(2.6)
P 		126.3
(-1.2)
P 		114.4
(-3)
A 		40
C 128.9
(1.5)
P 		154.6
(3)
P 		109.6
(0.4)
P 		52.3
(-3)
A 		102
avr 145.9
(2.5) 		151.5
(3) 		120.6
(0.2) 		86.4
(-3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 212
1 2 3 4 5 6 range
A 254.8
(3)
P 		149.5
(0)
P 		114.1
(-1)
P 		43
(-3)
A 		60.4
(-2.5)
P 		64
(-2.4)
A 		212
B 228.7
(3)
P 		194.4
(1.8)
P 		105.6
(-1.3)
P 		95.9
(-1.6)
P 		78.7
(-2.2)
A 		57.1
(-3)
M 		172
C 194
(3)
P 		151.7
(1)
P 		86.3
(-2.2)
P 		135.3
(0.2)
M 		69.5
(-3)
M 		88.6
(-2.1)
P 		125
avr 225.8
(3) 		165.2
(0.7) 		102
(-1.8) 		91.4
(-2.2) 		69.5
(-3) 		69.9
(-3) 		156
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 56.3
(3)
P 		32.3
(-0.4)
P 		32.9
(-0.3)
P 		13.5
(-3)
A 		43
B 49.1
(3)
P 		37.4
(0.2)
P 		29.3
(-1.8)
P 		24.4
(-3)
A 		25
C 42.7
(2.7)
P 		44.5
(3)
P 		38.2
(1.9)
P 		8.8
(-3)
A 		36
avr 49.4
(3) 		38.1
(1) 		33.5
(0.2) 		15.6
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 47
(2.1)
P 		53.2
(3)
P 		24.8
(-1)
P 		10.9
(-3)
A 		18.2
(-2)
P 		15.6
(-2.3)
A 		42
B 79.6
(3)
P 		64.7
(1.5)
P 		25.6
(-2.6)
P 		25.4
(-2.6)
P 		32
(-1.9)
A 		21.3
(-3)
M 		58
C 71.9
(3)
P 		46.3
(-0.1)
P 		22
(-3)
P 		27.2
(-2.4)
M 		22.5
(-2.9)
M 		29.7
(-2.1)
P 		50
avr 66.2
(3) 		54.7
(1.5) 		24.1
(-2.6) 		21.2
(-3) 		24.2
(-2.6) 		22.2
(-2.9) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185531_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2962
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.20 NCBI
Target Description Y53F4B.20 /REP_DB=WormBase Gene ID /WP=CE25399 /TR=Q9NAA0 /GB=CAB70109.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064704(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064704 NCBI Caenorhabditis elegans Y53F4B.20 (Y53F4B.20) mRNA, complete cds. 11/11 None
GENEFINDER00000043131 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15084366:15089542:1 11/11 None
Y53F4B.20 ENSEMBL cdna:known chromosome:CEL140:II:15084366:15089542:1 gene:Y53F4B.20 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043054 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15084362-15089539(+) 98.96 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.20
Entrez Gene 175160 Entrez gene
SwissProt Q9NAA0 EMBL-EBI
WormBase CE35674 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4179 membrane alanyl aminopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB70109 Hypothetical protein Y53F4B.20 [Caenorhabditis elegans] ref|NP_497105.2| Y53F4B.20 [Caenorhabditis elegans] 0.0
blast CAE73455 Hypothetical protein CBG20905 [Caenorhabditis briggsae] 0.0
blast NP_500831 T12E12.6 [Caenorhabditis elegans] 1.0E-60
blast NP_500831 T12E12.6 [Caenorhabditis elegans] 8.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 6.8E-8
Sequence
Target Sequence 
>C. ELEGANS:185531_AT
gaagttcaccgaattcaagctgcccatccaaataatggtcagccacgggatccacctctc
gccaattcgagatggcctaattttttcgaatttgggatttacgaactattatcgtgtcaa
ttatgacattgacacgtggcgggagatcaaaactgtgctcactgaaaatgccacgtcata
cacgctacgagagcggtttcagctcgtttcagatttttgctatttttattcgattaaatc
gctgcccgagccagcagcctcagtgctccgcaacgaattcgtgcagctcgtccgtctccg
gccgacgtcgttcccgatctgcgatgcggcgatcttccagtgcgtggtgactcatgagca
cacgaagccacgacatcttgacaagagcacgatggttcagttgaggcggaaggttttcga
ctcgttcgcgaattcttcggaaatggagtgccgcgccgggctcgcacatgatgctcttaa
tg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTTCACCGAATTCAAGCTGCCC 189 337 1608 Antisense
AATAATGGTCAGCCACGGGATCCAC 358 171 1638 Antisense
ATCCACCTCTCGCCAATTCGAGATG 167 41 1657 Antisense
GCCACGTCATACACGCTACGAGAGC 409 279 1777 Antisense
GTTTCAGCTCGTTTCAGATTTTTGC 547 449 1803 Antisense
TTAAATCGCTGCCCGAGCCAGCAGC 2 683 1841 Antisense
TCAGTGCTCCGCAACGAATTCGTGC 687 623 1867 Antisense
GATCTTCCAGTGCGTGGTGACTCAT 431 419 1938 Antisense
GACTCATGAGCACACGAAGCCACGA 27 375 1956 Antisense
GAAGCCACGACATCTTGACAAGAGC 475 345 1971 Antisense
GGGCTCGCACATGATGCTCTTAATG 294 493 2065 Antisense
Sequence Source Affymetrix Proprietary Database