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[185527_at]

Description Y53F4B.24 /REP_DB=WormBase Gene ID /WP=CE24419 /TR=Q9NAB6 /GB=CAB61098.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 51      4grp_total > 42      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 2.8
(-2.8)
A 		3.3
(-2.4)
A 		2.6
(-3)
A 		9.3
(3)
A 		7
B 5
(-1.9)
A 		18
(3)
A 		2.1
(-3)
A 		3.8
(-2.4)
A 		16
C 2.6
(-2.9)
A 		3.6
(-2.2)
A 		10.8
(3)
A 		2.5
(-3)
A 		8
avr 3.5
(-3) 		8.3
(3) 		5.2
(-0.9) 		5.2
(-0.9) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 3
(-2.4)
A 		1.5
(-3)
A 		1.7
(-2.9)
A 		17.5
(3)
A 		2.2
(-2.7)
A 		2.3
(-2.7)
A 		16
B 2.4
(3)
A 		2.4
(3)
A 		1.2
(-2.5)
A 		1.7
(-0.2)
A 		1.9
(0.7)
A 		1.1
(-3)
A 		1
C 2.2
(-2.6)
A 		2.2
(-2.6)
A 		26.3
(3)
A 		2.1
(-2.7)
A 		0.7
(-3)
A 		2
(-2.7)
A 		26
avr 2.5
(-2.3) 		2
(-2.7) 		9.7
(3) 		7.1
(1.1) 		1.6
(-3) 		1.8
(-2.9) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 1
(-0.4)
A 		0.7
(-3)
A 		0.7
(-3)
A 		1.4
(3)
A 		1
B 1.6
(-1.3)
A 		4.4
(3)
A 		0.5
(-3)
A 		0.8
(-2.5)
A 		4
C 0.9
(-2.1)
A 		1
(-1.9)
A 		3.8
(3)
A 		0.4
(-3)
A 		3
avr 1.2
(-1.4) 		2
(3) 		1.7
(1.4) 		0.9
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 0.6
(-2.7)
A 		0.5
(-2.9)
A 		0.4
(-3)
A 		4.4
(3)
A 		0.7
(-2.6)
A 		0.6
(-2.7)
A 		4
B 0.9
(3)
A 		0.8
(2)
A 		0.3
(-3)
A 		0.4
(-2)
A 		0.8
(2)
A 		0.4
(-2)
A 		1
C 0.8
(-2.4)
A 		0.7
(-2.5)
A 		6.7
(3)
A 		0.4
(-2.8)
A 		0.2
(-3)
A 		0.7
(-2.5)
A 		7
avr 0.8
(-2.4) 		0.7
(-2.7) 		2.5
(3) 		1.7
(0.5) 		0.6
(-3) 		0.6
(-3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185527_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3515
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.24 NCBI
Target Description Y53F4B.24 /REP_DB=WormBase Gene ID /WP=CE24419 /TR=Q9NAB6 /GB=CAB61098.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064709(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064709 NCBI Caenorhabditis elegans Y53F4B.24 (Y53F4B.24) mRNA, complete cds. 11/11 None
GENEFINDER00000043124 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15126464:15133911:-1 11/11 None
Y53F4B.24 ENSEMBL cdna:known chromosome:CEL140:II:15126464:15133911:-1 gene:Y53F4B.24 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043058 6/11 Cross Hyb Matching Probes None
SNAP00000043059 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15126460-15133908(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.24
Entrez Gene 190221 Entrez gene
SwissProt Q9NAB6 EMBL-EBI
WormBase CE24419 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB61098 Hypothetical protein Y53F4B.24 [Caenorhabditis elegans] ref|NP_497110.1| Y53F4B.24 [Caenorhabditis elegans] 0.0
blast CAE59715 Hypothetical protein CBG03147 [Caenorhabditis briggsae] 3.0E-58
blast CAB07367 Hypothetical protein F22G12.3 [Caenorhabditis elegans] ref|NP_493304.1| F22G12.3 [Caenorhabditis elegans] 5.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 5.8E-5
Sequence
Target Sequence 
>C. ELEGANS:185527_AT
gaccctcgtgagcaatagatcggatttcgagtacaccaacgatgcaattagtttgaagtc
tgcccatggctacctcaacctctccccatctgaacctgacgccacactcgaccgaactca
aactgaggagaactgctcaatgaactattatacccacagggtgcaacagaacattaactc
taaccgctcgtccacctgcattgctccaacattccgctcaaccatctcccgtgcttcagt
catttccctccgccaaaacctgctcgaaagtcaccaagctcatgctcaaaatgctccggc
tcttcctcaagccccaccaccaaactcgattcgccaaggagcccttcgtgtagcctatct
ggcaaagaagtttgagtgcaagaaatcggggaaaaagtctgaaaattccgagaaaattcg
ttccccaccactggaagttctgttcccgccgctaccggttcaacaactgcaggaggagga
tttgaagagcttgaaaagcctgaaaagccctagagtctccaagctggattttactccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCCTCGTGAGCAATAGATCGGAT 568 381 2025 Antisense
CGTGAGCAATAGATCGGATTTCGAG 557 245 2031 Antisense
ACTGCTCAATGAACTATTATACCCA 157 99 2156 Antisense
AATGAACTATTATACCCACAGGGTG 494 153 2163 Antisense
ACCCACAGGGTGCAACAGAACATTA 544 83 2176 Antisense
CAGAACATTAACTCTAACCGCTCGT 553 191 2191 Antisense
CCCTTCGTGTAGCCTATCTGGCAAA 329 259 2366 Antisense
GTAGCCTATCTGGCAAAGAAGTTTG 548 457 2374 Antisense
GAGTGCAAGAAATCGGGGAAAAAGT 24 399 2398 Antisense
CCCTAGAGTCTCCAAGCTGGATTTT 80 259 2532 Antisense
GTCTCCAAGCTGGATTTTACTCCAT 145 471 2539 Antisense
Sequence Source Affymetrix Proprietary Database