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[185526_at]

Description Y50E8A.14 /REP_DB=WormBase Gene ID /WP=CE24403 /TR=Q9U274 /GB=CAB60585.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 42      4grp_total > 62      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 26
(3)
A 		3.3
(-3)
A 		6.9
(-2)
A 		5.6
(-2.4)
A 		23
B 2.2
(-3)
A 		9.3
(3)
A 		3.7
(-1.7)
A 		2.7
(-2.6)
A 		7
C 2.3
(-3)
A 		5.9
(-1.9)
A 		22.4
(3)
A 		3.6
(-2.6)
A 		20
avr 10.2
(2.3) 		6.2
(-1.1) 		11
(3) 		4
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 2.5
(-2.4)
A 		3.2
(-1.4)
A 		6.2
(3)
A 		5
(1.2)
A 		2.1
(-3)
A 		2.3
(-2.7)
A 		4
B 3
(3)
A 		2.6
(1.4)
A 		2.2
(-0.2)
A 		1.5
(-3)
A 		2.3
(0.2)
A 		1.8
(-1.8)
A 		2
C 7.1
(3)
A 		2.9
(-1.5)
A 		5.6
(1.4)
A 		4
(-0.3)
A 		1.7
(-2.8)
A 		1.5
(-3)
A 		6
avr 4.2
(1.9) 		2.9
(-0.9) 		4.7
(3) 		3.5
(0.4) 		2
(-2.8) 		1.9
(-3) 		3
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 9.5
(3)
A 		0.7
(-3)
A 		1.8
(-2.3)
A 		0.8
(-2.9)
A 		9
B 0.7
(-2.6)
A 		2.3
(3)
A 		0.9
(-1.9)
A 		0.6
(-3)
A 		2
C 0.7
(-2.9)
A 		1.7
(-2.1)
A 		7.8
(3)
A 		0.6
(-3)
A 		7
avr 3.6
(3) 		1.6
(-1.1) 		3.5
(2.8) 		0.7
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2
1 2 3 4 5 6 range
A 0.5
(-3)
A 		1.1
(1)
A 		1.4
(3)
A 		1.3
(2.3)
A 		0.6
(-2.3)
A 		0.6
(-2.3)
A 		1
B 1.1
(3)
A 		0.9
(1.3)
A 		0.5
(-2.1)
A 		0.4
(-3)
A 		0.9
(1.3)
A 		0.7
(-0.4)
A 		1
C 2.6
(3)
A 		0.9
(-1.9)
A 		1.4
(-0.4)
A 		0.8
(-2.1)
A 		0.6
(-2.7)
A 		0.5
(-3)
A 		2
avr 1.4
(3) 		1
(0) 		1.1
(0.8) 		0.8
(-1.5) 		0.7
(-2.3) 		0.6
(-3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185526_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12899
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.14 NCBI
Target Description Y50E8A.14 /REP_DB=WormBase Gene ID /WP=CE24403 /TR=Q9U274 /GB=CAB60585.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074243(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074243 NCBI Caenorhabditis elegans Y50E8A.14 (Y50E8A.14) mRNA, complete cds. 11/11 None
SNAP00000008990 ENSEMBL cdna:SNAP chromosome:CEL140:V:14767259:14768418:1 11/11 None
Y50E8A.14 ENSEMBL cdna:known chromosome:CEL140:V:14765069:14768418:1 gene:Y50E8A.14 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000009982 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Public Domain and Genome References
Gene Title
Ensembl Y50E8A.14
Entrez Gene 190113 Entrez gene
SwissProt Q9U274 EMBL-EBI
WormBase CE24403 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60585 Hypothetical protein Y50E8A.14 [Caenorhabditis elegans] ref|NP_506644.1| Y50E8A.14 [Caenorhabditis elegans] 0.0
blast T31617 hypothetical protein Y50E8A.m - Caenorhabditis elegans 0.0
blast AAF36060 Hypothetical protein Y75D11A.5 [Caenorhabditis elegans] ref|NP_508324.1| Y75D11A.5 [Caenorhabditis elegans] 1.0E-150
blast CAB60585 Hypothetical protein Y50E8A.14 [Caenorhabditis elegans] ref|NP_506644.1| Y50E8A.14 [Caenorhabditis elegans] 1.0E-142
blast T31617 hypothetical protein Y50E8A.m - Caenorhabditis elegans 1.0E-107
blast CAB54374 Hypothetical protein Y105C5B.26 [Caenorhabditis elegans] ref|NP_502915.1| Y105C5B.26 [Caenorhabditis elegans] 4.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR008042 EMBL-EBI Retrotransposon, Pao 1.2E-58
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 2.5E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506644.1 2 734-756,780-802
Y50E8A.14 2 734-756,780-802
SNAP00000008990 2 293-315,343-365
Sequence
Target Sequence 
>C. ELEGANS:185526_AT
gagttggcctctggcacgagttgaagaagtaggtcccagatcagcaaaactgtacgctgg
acacaccggaaattttatcgagagacctttcaagagcatatatccactcgaagtcagttt
caacaaggatgaagaaccagtgaacgagagcacaaacacggagactccgattccaacaaa
tgccatcaccaaggataatagaaccgatatggatgaagaaaatcagattgtaccaccaac
aagagatgacagttcccgctcaactactcgcagcggcaaaagctacggtatcagtaatgc
ggttactttggctctcattatgatggctgtaatgatcacgtccacaaatgcaactacgac
ggactacccactcacaacggacacaggcgctgagacgaaaactacgactggaagcaatcc
ataccatcttctttgggctattccaattgccttcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTTGGCCTCTGGCACGAGTTGAA 542 397 1929 Antisense
GAAGTAGGTCCCAGATCAGCAAAAC 576 319 1954 Antisense
CAAAACTGTACGCTGGACACACCGG 125 189 1973 Antisense
GGAGACTCCGATTCCAACAAATGCC 464 519 2088 Antisense
ACAAGAGATGACAGTTCCCGCTCAA 444 113 2167 Antisense
GCTCAACTACTCGCAGCGGCAAAAG 630 301 2186 Antisense
GTAATGCGGTTACTTTGGCTCTCAT 521 461 2222 Antisense
GATGGCTGTAATGATCACGTCCACA 175 407 2250 Antisense
AAATGCAACTACGACGGACTACCCA 250 111 2274 Antisense
GCAATCCATACCATCTTCTTTGGGC 220 321 2342 Antisense
TTGGGCTATTCCAATTGCCTTCGGA 551 705 2361 Antisense
Sequence Source Affymetrix Proprietary Database