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[185524_at]

Description F35D11.5 /REP_DB=WormBase Gene ID /WP=CE02718 /TR=Q20033 /GB=AAA68751.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 54      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 73      6grp_total > 54      4grp_total > 57      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 566
1 2 3 4 range
A 565.4
(-2.5)
P 		980.5
(3)
P 		939.8
(2.5)
P 		524.4
(-3)
P 		456
B 586.7
(-1.7)
P 		858.7
(1.3)
P 		1013.8
(3)
P 		464.3
(-3)
P 		550
C 622
(-0.7)
P 		817.3
(1.9)
P 		901.7
(3)
P 		447.9
(-3)
P 		454
avr 591.4
(-1.6) 		885.5
(2.2) 		951.8
(3) 		478.9
(-3) 		473

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 284
1 2 3 4 5 6 range
A 424.3
(-3)
P 		563.3
(1.5)
P 		608.4
(3)
P 		576.6
(2)
P 		507.5
(-0.3)
P 		499.9
(-0.5)
P 		184
B 530.6
(-1.6)
P 		647.4
(1.4)
P 		691.3
(2.6)
P 		474.3
(-3)
P 		708.5
(3)
P 		649.4
(1.5)
P 		234
C 683.2
(3)
P 		593.7
(0)
P 		537.4
(-1.8)
P 		502.3
(-3)
P 		589.1
(-0.1)
P 		673
(2.7)
P 		181
avr 546
(-1.2) 		601.5
(2.3) 		612.4
(3) 		517.7
(-3) 		601.7
(2.3) 		607.4
(2.7) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 239
1 2 3 4 range
A 205.9
(1.6)
P 		212.8
(1.8)
P 		245.7
(3)
P 		76.8
(-3)
P 		169
B 187.1
(0.9)
P 		212.1
(2)
P 		234.9
(3)
P 		98.9
(-3)
P 		136
C 205.9
(0.3)
P 		235
(1)
P 		314.3
(3)
P 		75.5
(-3)
P 		239
avr 199.6
(0.8) 		220
(1.5) 		265
(3) 		83.7
(-3) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 78.2
(-3)
P 		200.3
(3)
P 		132.5
(-0.3)
P 		146.2
(0.3)
P 		153.1
(0.7)
P 		121.7
(-0.9)
P 		122
B 184.6
(-0.8)
P 		215.5
(0.3)
P 		167.9
(-1.4)
P 		125.6
(-3)
P 		287.8
(3)
P 		242.2
(1.3)
P 		162
C 253.2
(3)
P 		181.2
(0.2)
P 		136.8
(-1.6)
P 		101
(-3)
P 		190.6
(0.5)
P 		225.3
(1.9)
P 		152
avr 172
(0.3) 		199
(2.2) 		145.7
(-1.5) 		124.3
(-3) 		210.5
(3) 		196.4
(2) 		86
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185524_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4299
Sequence Type Exemplar sequence
Representative Public ID F35D11.5 NCBI
Target Description F35D11.5 /REP_DB=WormBase Gene ID /WP=CE02718 /TR=Q20033 /GB=AAA68751.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062409(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062409 NCBI Caenorhabditis elegans F35D11.5 (F35D11.5) mRNA, complete cds. 11/11 None
SNAP00000013538 ENSEMBL cdna:SNAP chromosome:CEL140:II:4601525:4602620:-1 11/11 None
GENEFINDER00000013551 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4601525:4602620:-1 11/11 None
F35D11.5.2 ENSEMBL cdna:known chromosome:CEL140:II:4601342:4602620:-1 gene:F35D11.5 11/11 None
F35D11.5.1 ENSEMBL cdna:known chromosome:CEL140:II:4601360:4602622:-1 gene:F35D11.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4601523-4601969(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35D11.5
Entrez Gene 173796 Entrez gene
SwissProt Q20033 EMBL-EBI
WormBase CE29789 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68751 Hypothetical protein F35D11.5 [Caenorhabditis elegans] ref|NP_494810.2| F35D11.5 [Caenorhabditis elegans] 2.0E-70
blast CAE67853 Hypothetical protein CBG13442 [Caenorhabditis briggsae] 7.0E-53
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494810.2 1 85-104
F35D11.5.2 1 85-104
F35D11.5.1 1 85-104
SNAP00000013538 1 85-104
GENEFINDER00000013551 1 85-104
Sequence
Target Sequence 
>C. ELEGANS:185524_AT
gaaggacaatgagatgctggaaacaatcgatattgaggatctaccacgaccacagaaacg
gttcgccaagcagtttgagaaggtgaatcaagagcgagtcaaggagattttcgcgaaaaa
ttacaagaatcacatctcatttgccgtacttttgggtgttgttatcggaatctactggta
tacaatgtattctgtgaaacaggagacattcctcgaagaaatcgacgaagaaatggccgc
tacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGACAATGAGATGCTGGAAACA 217 333 69 Antisense
TATTGAGGATCTACCACGACCACAG 629 665 99 Antisense
CCACGACCACAGAAACGGTTCGCCA 122 275 112 Antisense
AACGGTTCGCCAAGCAGTTTGAGAA 607 143 125 Antisense
GAAGGTGAATCAAGAGCGAGTCAAG 345 333 147 Antisense
GAGTCAAGGAGATTTTCGCGAAAAA 25 399 164 Antisense
ACAAGAATCACATCTCATTTGCCGT 152 113 191 Antisense
ATCTCATTTGCCGTACTTTTGGGTG 82 33 202 Antisense
GGGTGTTGTTATCGGAATCTACTGG 16 499 222 Antisense
AGGAGACATTCCTCGAAGAAATCGA 35 53 269 Antisense
AATCGACGAAGAAATGGCCGCTACG 157 169 288 Antisense
Sequence Source Affymetrix Proprietary Database