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[185519_at]

Description C25E10.9 /REP_DB=WormBase Gene ID /WP=CE06871 /TR=Q18158 /GB=AAA92314.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      4grp_highest_8_8 > 0      4grp_high_8_8 > 44      6grp_highest_nonorm > 35      6grp_total > 73      4grp_total > 52      6grp_highest_sub > 04      6grp_cgc > 76      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1199
1 2 3 4 range
A 1877.1
(0.7)
P 		2229.1
(3)
P 		1657.6
(-0.8)
P 		1321.5
(-3)
P 		908
B 1658.1
(-0.7)
P 		2311.2
(3)
P 		1685.6
(-0.6)
P 		1265.1
(-3)
P 		1046
C 2154.5
(2.4)
P 		2264.3
(3)
P 		1845.1
(0.8)
P 		1112.1
(-3)
P 		1152
avr 1896.6
(0.8) 		2268.2
(3) 		1729.4
(-0.1) 		1232.9
(-3) 		1035

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4397
1 2 3 4 5 6 range
A 1014.5
(-3)
P 		1910
(-1.7)
P 		4505.5
(2.1)
P 		5110.6
(3)
P 		3347.8
(0.4)
P 		2279.8
(-1.1)
P 		4096
B 1288.6
(-3)
P 		1534.4
(-2.6)
P 		5411.9
(3)
P 		4566.4
(1.8)
P 		3747.5
(0.6)
P 		2471.9
(-1.3)
P 		4123
C 1382.2
(-3)
P 		1571.7
(-2.7)
P 		4270.8
(1.5)
P 		5210.7
(3)
P 		3539.4
(0.4)
P 		2779.5
(-0.8)
P 		3829
avr 1228.4
(-3) 		1672
(-2.3) 		4729.4
(2.6) 		4962.6
(3) 		3544.9
(0.7) 		2510.4
(-0.9) 		3734
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 526
1 2 3 4 range
A 683.5
(3)
P 		483.9
(0.6)
P 		433.3
(-0.1)
P 		193.4
(-3)
P 		490
B 528.7
(2.2)
P 		570.8
(3)
P 		390.6
(-0.6)
P 		269.6
(-3)
P 		301
C 713.2
(3)
P 		651
(2.3)
P 		643.1
(2.2)
P 		187.5
(-3)
P 		526
avr 641.8
(3) 		568.6
(2) 		489
(0.8) 		216.8
(-3) 		425

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1336
1 2 3 4 5 6 range
A 187
(-3)
P 		679.3
(-0.3)
P 		981.2
(1.3)
P 		1296.2
(3)
P 		1010.2
(1.5)
P 		555.1
(-1)
P 		1109
B 448.4
(-3)
P 		510.7
(-2.7)
P 		1314.2
(1.8)
P 		1209.5
(1.3)
P 		1522.6
(3)
P 		921.8
(-0.4)
P 		1074
C 512.3
(-2.7)
P 		479.6
(-3)
P 		1087
(2.5)
P 		1047.9
(2.1)
P 		1145.3
(3)
P 		930.5
(1.1)
P 		666
avr 382.6
(-3) 		556.5
(-1.8) 		1127.5
(2.3) 		1184.5
(2.7) 		1226
(3) 		802.5
(-0) 		843
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185519_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12000
Sequence Type Exemplar sequence
Representative Public ID C25E10.9 NCBI
Target Description C25E10.9 /REP_DB=WormBase Gene ID /WP=CE06871 /TR=Q18158 /GB=AAA92314.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182364(11), NM_072945(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182364 NCBI Caenorhabditis elegans Inhibitor of Serine protease Like protein family member (isl-2) (isl-2) mRNA, complete cds. 11/11 None
NM_072945 NCBI Caenorhabditis elegans Inhibitor of Serine protease Like protein family member (isl-2) (isl-2) mRNA, complete cds. 11/11 None
C25E10.9b.4 ENSEMBL cdna:known chromosome:CEL140:V:9056855:9057370:-1 gene:C25E10.9 11/11 None
C25E10.9b.3 ENSEMBL cdna:known chromosome:CEL140:V:9056855:9057428:-1 gene:C25E10.9 11/11 None
C25E10.9b.2 ENSEMBL cdna:known chromosome:CEL140:V:9056855:9057428:-1 gene:C25E10.9 10/11 None
C25E10.9a ENSEMBL cdna:known chromosome:CEL140:V:9056855:9057419:-1 gene:C25E10.9 11/11 None
C25E10.9b.1 ENSEMBL cdna:known chromosome:CEL140:V:9056855:9057446:-1 gene:C25E10.9 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000038739 6/11 Cross Hyb Matching Probes None
GENEFINDER00000038747 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9056855-9057371(-) 99.51 99.51
Public Domain and Genome References
Ensembl C25E10.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA92314 Inhibitor of serine protease like protein protein 2, isoform a [Caenorhabditis elegans] ref|NP_505346.1| Inhibitor of Serine protease Like protein family member (isl-2) [Caenorhabditis elegans] 6.0E-81
blast CAE72086 Hypothetical protein CBG19174 [Caenorhabditis briggsae] 1.0E-64
blast AAA92313 Hypothetical protein C25E10.8 [Caenorhabditis elegans] ref|NP_505345.1| C25E10.8 [Caenorhabditis elegans] 2.0E-57
blast AAA92314 Inhibitor of serine protease like protein protein 2, isoform a [Caenorhabditis elegans] ref|NP_505346.1| Inhibitor of Serine protease Like protein family member (isl-2) [Caenorhabditis elegans] 8.0E-50
blast AAN84861 Inhibitor of serine protease like protein protein 2, isoform b [Caenorhabditis elegans] ref|NP_872164.1| Inhibitor of Serine protease Like protein family member (isl-2) [Caenorhabditis elegans] 8.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR002919 EMBL-EBI Trypsin inhibitor-like, cysteine-rich TIL region 8.3E-10
Pfam IPR002919 EMBL-EBI Trypsin inhibitor-like, cysteine-rich TIL region 2.4E-25
Pfam IPR002919 EMBL-EBI Trypsin inhibitor-like, cysteine-rich TIL region 1.8E-23
Pfam IPR002919 EMBL-EBI Trypsin inhibitor-like, cysteine-rich TIL region 2.4E-25
Sequence
Target Sequence 
>C. ELEGANS:185519_AT
gatacttgtcataatcacatgcatcgttgctgttgctaccgccacaaaaacatgtgaagc
caatgaggagctggtatcgtgccataatacatgtgaaccacaatgtggatatacccctaa
agcttgcacggagcaatgtatcatgaacacatgtgactgtaaagacggatttgttcgtaa
ttctcttggaaaatgcgtcgaagtatctgaatgcaccaaggaaactacaaaatgcccaga
aaatgagacattctttggctgtggaacagcttgtgaagcaacttgtgagaaaccaaatcc
aacagtttgcactaaacaatgtatcgtcaatgtctgccaatgctccaaaggtttcgttcg
tcatggactca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACTTGTCATAATCACATGCATC 572 423 18 Antisense
CACATGCATCGTTGCTGTTGCTACC 705 193 33 Antisense
CTGTTGCTACCGCCACAAAAACATG 496 239 47 Antisense
GAGGAGCTGGTATCGTGCCATAATA 173 405 82 Antisense
TACCCCTAAAGCTTGCACGGAGCAA 651 647 129 Antisense
GACGGATTTGTTCGTAATTCTCTTG 244 377 181 Antisense
GCGTCGAAGTATCTGAATGCACCAA 575 291 212 Antisense
GAGACATTCTTTGGCTGTGGAACAG 326 393 262 Antisense
GTATCGTCAATGTCTGCCAATGCTC 66 455 338 Antisense
GTCTGCCAATGCTCCAAAGGTTTCG 167 453 349 Antisense
AAAGGTTTCGTTCGTCATGGACTCA 432 119 364 Antisense
Sequence Source Affymetrix Proprietary Database