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[185518_at]

Description R12C12.6 /REP_DB=WormBase Gene ID /WP=CE02851 /TR=Q21960 /GB=AAC46785.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 82      4grp_highest_8_8 > 06      4grp_high_8_8 > 22      6grp_highest_nonorm > 65      6grp_total > 80      4grp_total > 25      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 362
1 2 3 4 range
A 882.7
(-3)
P 		996.9
(0.1)
P 		1107.3
(3)
P 		935.9
(-1.6)
P 		225
B 960.1
(-1.4)
P 		931.4
(-1.9)
P 		872.9
(-3)
P 		1199.5
(3)
P 		327
C 837.6
(-3)
P 		1062.5
(1.8)
P 		907.7
(-1.5)
P 		1121.6
(3)
P 		284
avr 893.5
(-3) 		996.9
(0.2) 		962.6
(-0.8) 		1085.7
(3) 		192

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 781
1 2 3 4 5 6 range
A 948.7
(-3)
P 		1169.3
(-1.1)
P 		1179.1
(-1)
P 		1649.1
(3)
P 		1088.6
(-1.8)
P 		1256.1
(-0.4)
P 		700
B 869.4
(-3)
P 		868
(-3)
P 		1045.5
(-0.7)
P 		1218.4
(1.5)
P 		1334.8
(3)
P 		922.7
(-2.3)
P 		467
C 1005.1
(-2)
P 		919.4
(-3)
P 		1147.2
(-0.4)
P 		1438.8
(3)
P 		1117
(-0.7)
P 		1155.8
(-0.3)
P 		519
avr 941.1
(-3) 		985.6
(-2.5) 		1123.9
(-0.8) 		1435.4
(3) 		1180.1
(-0.1) 		1111.5
(-0.9) 		494
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 321.4
(3)
P 		216.4
(-0.4)
P 		289.5
(2)
P 		137
(-3)
P 		184
B 306.2
(3)
P 		230
(-1.4)
P 		202.3
(-3)
P 		255.6
(0.1)
P 		104
C 277.3
(1.2)
P 		305.5
(2.5)
P 		316.4
(3)
P 		189.1
(-3)
P 		127
avr 301.6
(3) 		250.6
(0.2) 		269.4
(1.2) 		193.9
(-3) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 367
1 2 3 4 5 6 range
A 174.9
(-3)
P 		415.9
(2.9)
P 		256.8
(-1)
P 		418.3
(3)
P 		328.5
(0.8)
P 		305.8
(0.2)
P 		243
B 302.5
(-2)
P 		288.9
(-2.3)
P 		253.9
(-3)
P 		322.7
(-1.6)
P 		542.3
(3)
P 		344.1
(-1.1)
P 		288
C 372.5
(2.2)
P 		280.6
(-3)
P 		292
(-2.4)
P 		289.4
(-2.5)
P 		361.4
(1.6)
P 		386.9
(3)
P 		106
avr 283.3
(-2.3) 		328.5
(-0.4) 		267.6
(-3) 		343.5
(0.2) 		410.7
(3) 		345.6
(0.3) 		143
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185518_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5279
Sequence Type Exemplar sequence
Representative Public ID R12C12.6 NCBI
Target Description R12C12.6 /REP_DB=WormBase Gene ID /WP=CE02851 /TR=Q21960 /GB=AAC46785.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062806(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062806 NCBI Caenorhabditis elegans R12C12.6 (R12C12.6) mRNA, complete cds. 11/11 None
SNAP00000025496 ENSEMBL cdna:SNAP chromosome:CEL140:II:6046358:6047678:-1 11/11 None
GENEFINDER00000025591 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6046358:6047822:-1 11/11 None
R12C12.6 ENSEMBL cdna:known chromosome:CEL140:II:6045997:6047822:-1 gene:R12C12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6046354-6047819(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12C12.6
Entrez Gene 187840 Entrez gene
SwissProt Q21960 EMBL-EBI
WormBase CE02851 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1592916_AT similar to R12C12.6 cfa
CANINE:1588276_S_AT similar to R12C12.6 cfa
CANINE:1588275_AT similar to R12C12.6 cfa
CANINE_2:CFA.9768.1.S1_A_AT similar to R12C12.6 cfa
CANINE_2:CFA.9768.1.S1_AT similar to R12C12.6 cfa
CANINE_2:CFA.9768.2.S1_S_AT similar to R12C12.6 cfa
CHICKEN:GGA.4455.1.S1_AT similar to chromosome 11 open reading frame 15 gga
HU35KSUBA:AA215970_AT TMEM9 domain family, member B hs
HU35KSUBA:RC_AA433929_AT TMEM9 domain family, member B hs
HG-U133A:218065_S_AT TMEM9 domain family, member B hs
HG-U133_PLUS_2:218065_S_AT TMEM9 domain family, member B hs
HG-U133A_2:218065_S_AT TMEM9 domain family, member B hs
U133_X3P:G11034854_3P_A_AT TMEM9 domain family, member B hs
U133_X3P:HS.121619.0.S1_3P_A_AT TMEM9 domain family, member B hs
HG-U133B:222507_S_AT TMEM9 domain family, member B hs
HG-U133_PLUS_2:222507_S_AT TMEM9 domain family, member B hs
HG-U95B:56531_AT TMEM9 domain family, member B hs
MG-U74AV2:160434_AT TMEM9 domain family, member B mm
MG-U74CV2:171041_R_AT TMEM9 domain family, member B mm
MOE430A:1448867_AT TMEM9 domain family, member B mm
MOUSE430A_2:1448867_AT TMEM9 domain family, member B mm
MOUSE430_2:1448867_AT TMEM9 domain family, member B mm
MOE430A:1417874_AT TMEM9 domain family, member B mm
MOUSE430A_2:1417874_AT TMEM9 domain family, member B mm
MOUSE430_2:1417874_AT TMEM9 domain family, member B mm
MU19KSUBB:TC30768_AT RIKEN cDNA 2310004K06 gene, mRNA (cDNA clone MGC:7457 IMAGE:3490055) mm
RG-U34A:RC_AA893581_AT similar to D7H11orf15 protein (predicted) rn
RG-U34C:RC_AI103555_AT similar to D7H11orf15 protein (predicted) rn
RAE230A:1374058_AT similar to D7H11orf15 protein (predicted) rn
RAT230_2:1374058_AT similar to D7H11orf15 protein (predicted) rn
RAE230A:1374675_AT similar to D7H11orf15 protein (predicted) rn
RAT230_2:1374675_AT similar to D7H11orf15 protein (predicted) rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495207 R12C12.6 [Caenorhabditis elegans] gb|AAC46785.1| Hypothetical protein R12C12.6 [Caenorhabditis elegans] 1.0E-126
blast NP_495207 R12C12.6 [Caenorhabditis elegans] gb|AAC46785.1| Hypothetical protein R12C12.6 [Caenorhabditis elegans] 6.0E-98
blast CAE59242 Hypothetical protein CBG02568 [Caenorhabditis briggsae] 8.0E-88
blast CAE59242 Hypothetical protein CBG02568 [Caenorhabditis briggsae] 1.0E-87
blast EAA04310 ENSANGP00000016573 [Anopheles gambiae str. PEST] ref|XP_308543.2| ENSANGP00000016573 [Anopheles gambiae str. PEST] 1.0E-14
blast EAA04310 ENSANGP00000016573 [Anopheles gambiae str. PEST] ref|XP_308543.2| ENSANGP00000016573 [Anopheles gambiae str. PEST] 2.0E-14
Protein Domains
Database ID Description E-Value
Pfam IPR008853 EMBL-EBI TMEM9 1.0E-126
Pfam IPR008853 EMBL-EBI TMEM9 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495207.1 3 7-29,44-66,151-173
R12C12.6 3 7-29,44-66,151-173
SNAP00000025496 1 107-129
GENEFINDER00000025591 3 7-29,44-66,151-173
Sequence
Target Sequence 
>C. ELEGANS:185518_AT
cttcccgcattatcccaagcagggactgaggctaacttcgaagatacacgatgtcgatgc
atttgtccgagtcttttaaaatttctcgacttggctgaaaatacaactgaaaaaacagaa
ggattgaggagacgattttatacaaaaactaacatcgaaccgagccattgcaagccaagc
aacattgtgaaagatcaagtcagtaacttcgttgacgagacccatatggatgcatttctt
gcaaattgcgactgccgttatgagtcacgaaacactgtgctcttaaaagtagttgtaata
tttgtaatctgtgtgatcgctgttttaacgggatacatggtattccttatgtgcctggat
ccaatgctccggaagaaacgattatcgatcagttatcaacaacataatgatgaaatggag
gataatatctttgctgctgctccatcaactgacgatgaatcatcttcagcaagtaattca
atggatacacaaggaaccacacgtgcgagaagtaatgttctcggacgcgtcgaggccgaa
cagaatcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCCCGCATTATCCCAAGCAGGGA 273 219 187 Antisense
GCAGGGACTGAGGCTAACTTCGAAG 324 311 205 Antisense
GATGTCGATGCATTTGTCCGAGTCT 503 409 236 Antisense
GAACCGAGCCATTGCAAGCCAAGCA 261 345 343 Antisense
CTTCGTTGACGAGACCCATATGGAT 369 219 393 Antisense
TTGCGACTGCCGTTATGAGTCACGA 269 701 432 Antisense
GAGTCACGAAACACTGTGCTCTTAA 490 399 448 Antisense
ATCTGTGTGATCGCTGTTTTAACGG 663 33 493 Antisense
GGTATTCCTTATGTGCCTGGATCCA 662 505 525 Antisense
AGGATAATATCTTTGCTGCTGCTCC 287 57 605 Antisense
GACGCGTCGAGGCCGAACAGAATCG 286 381 710 Antisense
Sequence Source Affymetrix Proprietary Database