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[185514_at]

Description Y23H5A.4 /REP_DB=WormBase Gene ID /WP=CE18360 /TR=O76615 /GB=AAC64629.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 35      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 6.5
(-2.4)
A 		44.9
(3)
A 		2.6
(-3)
A 		8.2
(-2.2)
A 		42
B 5.1
(-3)
A 		10.9
(-0.3)
A 		6.5
(-2.3)
A 		17.9
(3)
A 		13
C 7.1
(3)
A 		5.6
(-0.9)
A 		5.6
(-0.9)
A 		4.8
(-3)
A 		2
avr 6.2
(-2.5) 		20.5
(3) 		4.9
(-3) 		10.3
(-0.9) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 7.2
(-3)
A 		26.9
(-1.9)
A 		7.9
(-2.9)
A 		7
(-3)
A 		61.5
(0)
A 		114.5
(3)
A 		108
B 16.7
(-1.9)
A 		5.3
(-2.8)
A 		2.3
(-3)
A 		36.7
(-0.4)
A 		62.9
(1.5)
A 		82.5
(3)
A 		80
C 3.8
(-3)
A 		3.6
(-3)
A 		4.9
(-2.9)
A 		47.1
(-0.3)
A 		52.1
(0)
A 		100.3
(3)
M 		97
avr 9.2
(-2.7) 		11.9
(-2.6) 		5
(-3) 		30.3
(-1.4) 		58.8
(0.4) 		99.1
(3) 		94
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 2.4
(-1.9)
A 		9.8
(3)
A 		0.7
(-3)
A 		1.2
(-2.7)
A 		9
B 1.6
(-2.7)
A 		2.7
(0.1)
A 		1.5
(-3)
A 		3.8
(3)
A 		2
C 2.3
(3)
A 		1.6
(0.2)
A 		2
(1.8)
A 		0.8
(-3)
A 		2
avr 2.1
(-1.7) 		4.7
(3) 		1.4
(-3) 		1.9
(-2.1) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 1.3
(-3)
A 		9.6
(-1.1)
A 		1.7
(-2.9)
A 		1.8
(-2.9)
A 		18.6
(0.9)
A 		27.9
(3)
A 		27
B 5.8
(-2)
A 		1.8
(-2.8)
A 		0.6
(-3)
A 		9.7
(-1.2)
A 		25.6
(2)
A 		30.8
(3)
A 		30
C 1.4
(-2.9)
A 		1.1
(-3)
A 		1.3
(-3)
A 		9.5
(-1.4)
A 		16.9
(-0.1)
A 		33.6
(3)
M 		33
avr 2.8
(-2.7) 		4.2
(-2.4) 		1.2
(-3) 		7
(-1.8) 		20.4
(0.9) 		30.8
(3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185514_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.239
Sequence Type Exemplar sequence
Representative Public ID Y23H5A.4 NCBI
Target Description Y23H5A.4 /REP_DB=WormBase Gene ID /WP=CE18360 /TR=O76615 /GB=AAC64629.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058609(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058609 NCBI Caenorhabditis elegans Y23H5A.4 (Y23H5A.4) mRNA, complete cds. 11/11 None
SNAP00000016935 ENSEMBL cdna:SNAP chromosome:CEL140:I:2617561:2620350:1 11/11 None
GENEFINDER00000016944 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2617561:2620350:1 11/11 None
Y23H5A.4 ENSEMBL cdna:known chromosome:CEL140:I:2617561:2620350:1 gene:Y23H5A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2617560-2620350(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y23H5A.4
Entrez Gene 189522 Entrez gene
SwissProt O76615 EMBL-EBI
WormBase CE18360 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_491010 Y23H5A.4 [Caenorhabditis elegans] gb|AAC64629.1| Hypothetical protein Y23H5A.4 [Caenorhabditis elegans] 0.0
blast CAE68999 Hypothetical protein CBG14988 [Caenorhabditis briggsae] 1.0E-117
blast CAB54441 Hypothetical protein Y48B6A.5 [Caenorhabditis elegans] ref|NP_496960.1| Y48B6A.5 [Caenorhabditis elegans] 1.0E-103
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.1E-21
Sequence
Target Sequence 
>C. ELEGANS:185514_AT
gacaccaacgataacccagttttctgaggatgactcgctgcaatacatattcccagtgga
agagaatttggaaatgagcacagcgtgcagtttcgtgccgtcacttgatatgcagagcac
ccagaatccgtctccatctactttgaaaactatgaaaatcgaggaaagccggaatttcca
gaccatgtcggcgatgacgttggcctgcggtggagctgcagcagatattgtcaccgtgcc
ggccgaccggatcacgtttgccgctggcaagctggaagaagttattcgagtcactaacgt
ctcgcaatcgcatattatgttcgctttgaagacaaatgccgacgcctacctcatcgctgc
tccaacttctggagttcttctgagcggacaaagcatgacaatccgtgttggagtcactgg
caactacttcgatgatttttcggatcctggagtttccattgataagctcgccatcgacta
tgctctgatccccagccgttcctactccaaattttcctccgagttcttctattcccagaa
ccgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACCAACGATAACCCAGTTTTCT 435 365 579 Antisense
GACTCGCTGCAATACATATTCCCAG 286 375 610 Antisense
GAGCACAGCGTGCAGTTTCGTGCCG 234 391 654 Antisense
GTTTCGTGCCGTCACTTGATATGCA 381 449 668 Antisense
CATGTCGGCGATGACGTTGGCCTGC 310 209 762 Antisense
CACTAACGTCTCGCAATCGCATATT 215 197 870 Antisense
CTTCTGGAGTTCTTCTGAGCGGACA 17 221 944 Antisense
TGGAGTCACTGGCAACTACTTCGAT 634 545 987 Antisense
TTCCATTGATAAGCTCGCCATCGAC 405 699 1032 Antisense
TCCAAATTTTCCTCCGAGTTCTTCT 280 557 1084 Antisense
GAGTTCTTCTATTCCCAGAACCGAC 560 397 1099 Antisense
Sequence Source Affymetrix Proprietary Database