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[185512_at]

Description F35H10.2 /REP_DB=WormBase Gene ID /WP=CE04502 /TR=Q20075 /GB=AAA81685.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 70      4grp_highest_8_8 > 61      4grp_high_8_8 > 18      6grp_highest_nonorm > 72      6grp_total > 82      4grp_total > 30      6grp_highest_sub > 04      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 427
1 2 3 4 range
A 566.6
(3)
P 		341.9
(-1.2)
P 		242.1
(-3)
P 		508
(1.9)
P 		325
B 462.3
(3)
P 		346.8
(0.7)
P 		155.2
(-3)
P 		424.1
(2.3)
P 		307
C 582.6
(3)
P 		306.5
(-1.7)
P 		228.8
(-3)
P 		365.5
(-0.7)
P 		354
avr 537.2
(3) 		331.7
(-0.8) 		208.7
(-3) 		432.5
(1.1) 		329

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 234
1 2 3 4 5 6 range
A 494.4
(2.3)
P 		300.2
(-3)
P 		519.6
(3)
P 		455.6
(1.2)
P 		432
(0.6)
P 		323.3
(-2.4)
P 		219
B 437.6
(-0)
P 		415.6
(-0.7)
P 		405.2
(-1)
P 		459.3
(0.7)
P 		534.5
(3)
P 		341.8
(-3)
P 		193
C 345.5
(-2.5)
P 		485.7
(3)
P 		423.9
(0.6)
P 		441.6
(1.3)
P 		422.7
(0.5)
P 		332.3
(-3)
P 		153
avr 425.8
(1.3) 		400.5
(0.1) 		449.6
(2.4) 		452.2
(2.5) 		463.1
(3) 		332.5
(-3) 		131
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 206.3
(3)
P 		74.2
(-2.5)
P 		63.3
(-3)
P 		74.4
(-2.5)
P 		143
B 147.4
(3)
P 		85.7
(-0.3)
P 		36
(-3)
P 		90.4
(-0.1)
P 		111
C 192.9
(3)
P 		88.1
(-1.8)
P 		79.7
(-2.2)
P 		61.6
(-3)
P 		131
avr 182.2
(3) 		82.7
(-1.9) 		59.7
(-3) 		75.5
(-2.2) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 139
1 2 3 4 5 6 range
A 91.1
(-1.6)
P 		106.8
(0.3)
P 		113.2
(1)
P 		115.6
(1.3)
P 		130.4
(3)
P 		78.7
(-3)
P 		52
B 152.2
(-0.3)
P 		138.3
(-1)
P 		98.4
(-3)
P 		121.6
(-1.8)
P 		217.2
(3)
P 		127.4
(-1.5)
P 		119
C 128.1
(1)
P 		148.2
(3)
P 		107.9
(-1.1)
P 		88.8
(-3)
P 		136.8
(1.8)
P 		111.2
(-0.7)
P 		59
avr 123.8
(-1.1) 		131.1
(-0.3) 		106.5
(-2.9) 		108.7
(-2.7) 		161.5
(3) 		105.8
(-3) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185512_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10227
Sequence Type Exemplar sequence
Representative Public ID F35H10.2 NCBI
Target Description F35H10.2 /REP_DB=WormBase Gene ID /WP=CE04502 /TR=Q20075 /GB=AAA81685.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069001(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069001 NCBI Caenorhabditis elegans F35H10.2 (F35H10.2) mRNA, complete cds. 11/11 None
SNAP00000039429 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8329207:8329813:1 11/11 None
GENEFINDER00000039440 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8329207:8329813:1 11/11 None
F35H10.2 ENSEMBL cdna:known chromosome:CEL140:IV:8329207:8329813:1 gene:F35H10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8329212-8329819(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35H10.2
Entrez Gene 185328 Entrez gene
SwissProt Q20075 EMBL-EBI
WormBase CE04502 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81685 Hypothetical protein F35H10.2 [Caenorhabditis elegans] ref|NP_501402.1| F35H10.2 [Caenorhabditis elegans] 6.0E-93
blast CAE58458 Hypothetical protein CBG01598 [Caenorhabditis briggsae] 3.0E-60
Sequence
Target Sequence 
>C. ELEGANS:185512_AT
atgttcaacacacgcatttttctcctctcgttggtcattcttggagcatgttccgcagat
caaactgcaaattatgcggttcatcaaataatgaaaattgttgaaatagctgttgatcgt
caagaaatcagtccattactttaccttttggatccatctttcaactatacaatgtgtgga
tatgagggaaacgtgaagacttttaaaggatatttggagaatcaattggaaaatattatg
gatattaagttcttggtagactatgacaatgccgatgttgtaagaaatgggaatttggaa
actcttaaattcaacgtggacacaacaaccttgtataaagatagatctcgtgtggaaggt
ggagcagttgtgactgcgattcgtgagaaatactctgcgaatttctatatttcacatatc
tatcaacgttgccgtgttacctccaaacgtcaccatcgtctttatcgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTCAACACACGCATTTTTCTCC 450 47 13 Antisense
TCCTCTCGTTGGTCATTCTTGGAGC 111 593 35 Antisense
GGAGCATGTTCCGCAGATCAAACTG 378 521 55 Antisense
GAAATCAGTCCATTACTTTACCTTT 339 363 136 Antisense
ATTACTTTACCTTTTGGATCCATCT 422 13 147 Antisense
TGGATCCATCTTTCAACTATACAAT 219 551 161 Antisense
GGTAGACTATGACAATGCCGATGTT 660 503 267 Antisense
GTGGAAGGTGGAGCAGTTGTGACTG 540 491 364 Antisense
TACTCTGCGAATTTCTATATTTCAC 183 647 403 Antisense
TCTATCAACGTTGCCGTGTTACCTC 125 615 431 Antisense
AAACGTCACCATCGTCTTTATCGTC 88 127 457 Antisense
Sequence Source Affymetrix Proprietary Database