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[185507_at]

Description AH9.2 /REP_DB=WormBase Gene ID /WP=CE03858 /TR=SW:Q10905 /GB=AAA82440.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 49      4grp_highest_8_8 > 04      4grp_high_8_8 > 37      6grp_total > 39      4grp_total > 34      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 96.2
(-3)
P 		145.5
(3)
P 		118.2
(-0.3)
P 		130.4
(1.2)
P 		49
B 101.3
(-3)
P 		119.7
(2.9)
P 		102.7
(-2.5)
P 		119.9
(3)
P 		19
C 106.2
(2.8)
P 		106.6
(3)
P 		94.4
(-1.8)
P 		91.3
(-3)
P 		15
avr 101.2
(-3) 		123.9
(3) 		105.1
(-2) 		113.9
(0.4) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 176.3
(3)
P 		129.5
(-0.2)
P 		88.6
(-3)
P 		143.3
(0.7)
P 		141.3
(0.6)
P 		143.3
(0.7)
P 		88
B 177.4
(2)
P 		140.5
(-0.2)
P 		106.2
(-2.4)
P 		96.2
(-3)
P 		163
(1.1)
P 		192.8
(3)
P 		97
C 193.1
(3)
P 		160.8
(1)
P 		98.5
(-3)
P 		129.2
(-1.1)
A 		137.6
(-0.5)
P 		190.1
(2.8)
P 		95
avr 182.3
(3) 		143.6
(0.3) 		97.8
(-3) 		122.9
(-1.2) 		147.3
(0.5) 		175.4
(2.5) 		85
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 35
(3)
P 		31.6
(1.7)
P 		30.9
(1.5)
P 		19.1
(-3)
P 		16
B 32.3
(3)
P 		29.6
(1.1)
P 		23.8
(-3)
P 		25.6
(-1.7)
P 		9
C 35.2
(3)
P 		30.7
(1.6)
P 		32.9
(2.3)
P 		15.4
(-3)
P 		20
avr 34.2
(3) 		30.6
(1.5) 		29.2
(0.9) 		20
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 32.5
(-0)
P 		46.1
(3)
P 		19.3
(-3)
P 		36.3
(0.8)
P 		42.6
(2.2)
P 		34.9
(0.5)
P 		27
B 61.7
(1.7)
P 		46.8
(-0.2)
P 		25.8
(-3)
P 		25.5
(-3)
P 		66.2
(2.3)
P 		71.9
(3)
P 		46
C 71.6
(3)
P 		49.1
(0.1)
P 		25.1
(-3)
P 		26
(-2.9)
A 		44.5
(-0.5)
P 		63.6
(2)
P 		47
avr 55.3
(2.7) 		47.3
(1.3) 		23.4
(-3) 		29.3
(-1.9) 		51.1
(2) 		56.8
(3) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185507_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18443
Sequence Type Exemplar sequence
Representative Public ID AH9.2 NCBI
Target Description AH9.2 /REP_DB=WormBase Gene ID /WP=CE03858 /TR=SW:Q10905 /GB=AAA82440.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076014(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076014 NCBI Caenorhabditis elegans Cell-death-Related Nuclease family member (crn-4) (crn-4) mRNA, complete cds. 11/11 None
SNAP00000004860 ENSEMBL cdna:SNAP chromosome:CEL140:X:2256083:2258067:-1 11/11 None
GENEFINDER00000004866 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2256083:2258067:-1 11/11 None
AH9.2 ENSEMBL cdna:known chromosome:CEL140:X:2256083:2258074:-1 gene:AH9.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2256082-2258067(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol crn-4
Ensembl AH9.2
Entrez Gene 180536 Entrez gene
SwissProt Q10905 EMBL-EBI
WormBase CE03858 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4527 exonuclease activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA82440 Cell-death-related nuclease protein 4 [Caenorhabditis elegans] ref|NP_508415.1| Cell-death-Related Nuclease family member (crn-4) [Caenorhabditis elegans] gb|AAP57300.1| cell death-related nuclease 4 [Caenorhabditis elegans] sp|Q10905|CRN4_CAEEL Cell death-related nuclease 4 precursor 1.0E-177
blast CAE68330 Hypothetical protein CBG14049 [Caenorhabditis briggsae] 6.0E-79
blast CAE68329 Hypothetical protein CBG14047 [Caenorhabditis briggsae] 6.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR006055 EMBL-EBI Exonuclease 1.1E-35
Sequence
Target Sequence 
>C. ELEGANS:185507_AT
gagcagttccagcagtggctcatcacgcttggattggaagaaggaaagttcgctttcgtc
tgtgacagtcgtcaggatttgtggcgtattgctcagtatcagatgaaactgtccaatatc
caaatgccagctttcttccgtcagtacatcaatttgtacaagattttcacgaatgagatg
gatcgaatgggccccaaagagctttctgcgacgaccaacatcggcaagatgaacgaatac
tacgatctgccaaccatcggacgtgctcatgatgccatggatgactgtctgaatattgct
actattctccagcgaatgattaacatgggcgcaaaagttactgtgaacgagctgctgacc
tgttgtgcttcgtggcgcagacaaccgctggtctacaacaaagaatggagaagcagcttc
atggacgctggaaagattttcgaaagagtcctgccacttgtcgtgactacaattcgcgca
ggcgactttcgtctcgagatgtacggagtctgccgctattgccgcaaaggaatggatgtg
tgtggaacaagtcatcagcagacaccacatgatctctac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCAGTTCCAGCAGTGGCTCATCA 437 389 280 Antisense
AAAGTTCGCTTTCGTCTGTGACAGT 220 123 324 Antisense
CTTTCTTCCGTCAGTACATCAATTT 349 223 410 Antisense
TTTCTGCGACGACCAACATCGGCAA 137 669 482 Antisense
GAACGAATACTACGATCTGCCAACC 190 347 510 Antisense
TGAACGAGCTGCTGACCTGTTGTGC 28 575 623 Antisense
TTTCGAAAGAGTCCTGCCACTTGTC 327 669 717 Antisense
TCGTGACTACAATTCGCGCAGGCGA 25 605 740 Antisense
GGCGACTTTCGTCTCGAGATGTACG 595 541 760 Antisense
GATGTACGGAGTCTGCCGCTATTGC 378 407 777 Antisense
TCAGCAGACACCACATGATCTCTAC 677 623 834 Antisense
Sequence Source Affymetrix Proprietary Database