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[185505_s_at]

Description Y54E2A.12 /REP_DB=WormBase Gene ID /WP=CE20311 /TR=Q9XWI2 /GB=CAA21685.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 27      4grp_total > 04      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 119
(-0.6)
A 		77
(-3)
A 		89.6
(-2.3)
A 		180
(3)
A 		103
B 82
(-0.5)
A 		106
(3)
A 		64.6
(-3)
A 		103.7
(2.7)
A 		41
C 109.4
(0.5)
A 		87.9
(-3)
A 		125
(3)
A 		116
(1.5)
A 		37
avr 103.5
(-1.2) 		90.3
(-3) 		93.1
(-2.6) 		133.2
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 167.3
(3)
A 		68.6
(-2.2)
A 		72.8
(-2)
A 		54.1
(-3)
A 		71.3
(-2.1)
A 		108.4
(-0.1)
M 		113
B 79
(1.1)
A 		101
(3)
A 		56.3
(-0.9)
A 		31.9
(-3)
A 		41.9
(-2.1)
A 		76.4
(0.9)
A 		69
C 115.4
(3)
A 		69.9
(-1.6)
A 		71.8
(-1.4)
A 		113.9
(2.8)
A 		56.1
(-3)
A 		79.2
(-0.7)
A 		59
avr 120.6
(3) 		79.8
(-0.8) 		67
(-2) 		66.6
(-2) 		56.4
(-3) 		88
(-0) 		64
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 43.3
(3)
A 		16.7
(-3)
A 		23.4
(-1.5)
A 		26.4
(-0.8)
A 		27
B 26.2
(3)
A 		26.2
(3)
A 		15
(-3)
A 		22.1
(0.8)
A 		11
C 36.2
(1.2)
A 		25.3
(-1.6)
A 		43.6
(3)
A 		19.6
(-3)
A 		24
avr 35.2
(3) 		22.7
(-3) 		27.3
(-0.8) 		22.7
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 30.8
(3)
A 		24.4
(0.8)
A 		15.9
(-2.2)
A 		13.7
(-3)
A 		21.5
(-0.3)
A 		26.4
(1.5)
M 		17
B 27.5
(1.5)
A 		33.6
(3)
A 		13.7
(-1.7)
A 		8.4
(-3)
A 		17
(-1)
A 		28.5
(1.8)
A 		25
C 42.8
(3)
A 		21.3
(-2.2)
A 		18.3
(-3)
A 		22.9
(-1.9)
A 		18.2
(-3)
A 		26.5
(-1)
A 		25
avr 33.7
(3) 		26.4
(0.7) 		16
(-2.7) 		15
(-3) 		18.9
(-1.7) 		27.1
(0.9) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185505_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2738
Sequence Type Exemplar sequence
Representative Public ID Y54E2A.12 NCBI
Target Description Y54E2A.12 /REP_DB=WormBase Gene ID /WP=CE20311 /TR=Q9XWI2 /GB=CAA21685.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064667(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064667 NCBI Caenorhabditis elegans Y54E2A.12 (Y54E2A.12) mRNA, complete cds. 10/11 None
Y54E2A.12 ENSEMBL cdna:known chromosome:CEL140:II:14798362:14800795:1 gene:Y54E2A.12 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14798372-14800537(+) 97.76 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54E2A.12
Entrez Gene 175139 Entrez gene
SwissProt Q9XWI2 EMBL-EBI
WormBase CE37068 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.11328.1.S1_AT similar to TBC1 domain family, member 20 cfa
CHICKEN:GGAAFFX.3866.1.S1_AT similar to chromosome 20 open reading frame 140 gga
HU35KSUBB:RC_W73939_AT TBC1 domain family, member 20 hs
HU35KSUBB:RC_N74353_AT TBC1 domain family, member 20 hs
HU35KSUBC:RC_N63499_AT TBC1 domain family, member 20 hs
HU35KSUBC:RC_AA435762_AT TBC1 domain family, member 20 hs
U133_X3P:HS.167827.0.S1_3P_AT TBC1 domain family, member 20 hs
U133_X3P:HS.16475.0.S1_3P_AT TBC1 domain family, member 20 hs
HG-U133_PLUS_2:226409_AT TBC1 domain family, member 20 hs
HG-U133B:226409_AT TBC1 domain family, member 20 hs
HG-U133_PLUS_2:226664_AT TBC1 domain family, member 20 hs
HG-U133B:226664_AT TBC1 domain family, member 20 hs
HG-U95B:45812_AT TBC1 domain family, member 20 hs
HG-U95B:58325_AT TBC1 domain family, member 20 hs
HG-U95B:48658_AT TBC1 domain family, member 20 hs
HU35KSUBC:RC_H77371_AT TBC1 domain family, member 20 hs
MOUSE430_2:1426644_AT TBC1 domain family, member 20 mm
MOUSE430A_2:1426644_AT TBC1 domain family, member 20 mm
MOE430A:1426644_AT TBC1 domain family, member 20 mm
MOUSE430_2:1452140_AT TBC1 domain family, member 20 mm
MOUSE430A_2:1452140_AT TBC1 domain family, member 20 mm
MOE430A:1452140_AT TBC1 domain family, member 20 mm
MG-U74BV2:105484_AT TBC1 domain family, member 20 mm
MG-U74BV2:105431_AT TBC1 domain family, member 20 mm
MOE430B:1447688_AT TBC1 domain family, member 20 mm
MOUSE430_2:1447688_AT TBC1 domain family, member 20 mm
MU11KSUBA:AA673970_S_AT TBC1 domain family, member 20, mRNA (cDNA clone IMAGE:3489034) mm
MG-U74AV2:101505_AT TBC1 domain family, member 20, mRNA (cDNA clone IMAGE:3489034) mm
MU19KSUBA:TC18457_AT TBC1 domain family, member 20, mRNA (cDNA clone IMAGE:3489034) mm
RAE230B:1395785_AT Similar to chromosome 20 open reading frame 140 rn
RAT230_2:1395785_AT Similar to chromosome 20 open reading frame 140 rn
YEAST_2:1775387_AT GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Sc
Protein Similarities
Method ID Description E-Value
blast CAA21685 Hypothetical protein Y54E2A.12 [Caenorhabditis elegans] ref|NP_497068.2| Y54E2A.12 [Caenorhabditis elegans] 0.0
blast CAE60606 Hypothetical protein CBG04246 [Caenorhabditis briggsae] 0.0
blast XP_684605 PREDICTED: similar to TBC1 domain family, member 20 [Danio rerio] 2.0E-44
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497068.2 2 290-312,385-404
Y54E2A.12 2 290-312,385-404
Sequence
Target Sequence 
>C. ELEGANS:185505_S_AT
tttgcggcgaggtagatgctctcccagtctgctcaaatctcgcgaaaaatggctcattca
acaactatttgctaaaaactctcgaaaaatccgtagtccgagagctggatctcctctacg
tcatcttgtcacgtgtcgacccatctttggagcaagttatgcgatccgtcgagctcggca
ccatgttcggactctcgtggccgttgacctggttctcacatacactgaagcaatatcagc
agattgttcgatttttcgacgtcttcctcgcctcctcgccactgcttccgatctacgtgt
cggcggctgtcgtcgtgtttcgacgggcaagcattctggcttgtgagcgagaaatgccat
ttctacatcgtctgctcaccgaaatgccccacgagttgcccatcgacacgatcatcaagg
attctgtctttttgaatggaattatggttttgcaagccctgtcgatttacgaaaaatttt
gtactacacgtggacaagtctcaaagccatcgaaagccgccgtgaaaaccatcccaagat
acgcgcttcagttcatgttcgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGCGGCGAGGTAGATGCTCTCCC 279 669 683 Antisense
CCAGTCTGCTCAAATCTCGCGAAAA 626 271 706 Antisense
TACGTCATCTTGTCACGTGTCGACC 99 649 799 Antisense
GTGTCGACCCATCTTTGGAGCAAGT 249 485 815 Antisense
GCAAGTTATGCGATCCGTCGAGCTC 276 321 834 Antisense
TGACCTGGTTCTCACATACACTGAA 146 571 887 Antisense
GAAATGCCATTTCTACATCGTCTGC 594 361 1033 Antisense
AGTTGCCCATCGACACGATCATCAA 323 65 1076 Antisense
TTTTGCAAGCCCTGTCGATTTACGA 24 675 1130 Antisense
GAAAACCATCCCAAGATACGCGCTT 146 353 1206 Antisense
ATACGCGCTTCAGTTCATGTTCGTC 240 23 1221 Antisense
Sequence Source Affymetrix Proprietary Database