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[185495_at]

Description C25E10.4 /REP_DB=WormBase Gene ID /WP=CE06866 /TR=Q18153 /GB=AAA92308.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 90      6grp_total > 03      4grp_total > 50      6grp_highest_sub > 04      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 129.1
(-1.4)
P 		204.8
(3)
P 		165.9
(0.8)
P 		100.9
(-3)
P 		104
B 151
(-0.8)
P 		211.7
(3)
P 		164.9
(0.1)
P 		114.9
(-3)
P 		97
C 173.8
(-3)
P 		182
(-1)
P 		195.9
(2.5)
P 		198
(3)
P 		24
avr 151.3
(-1.7) 		199.5
(3) 		175.6
(0.7) 		137.9
(-3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 136
1 2 3 4 5 6 range
A 130.3
(0.2)
P 		106.3
(-1.2)
P 		108.8
(-1.1)
P 		175.6
(3)
P 		103.7
(-1.4)
P 		77.6
(-3)
P 		98
B 100
(1.7)
P 		111.7
(3)
P 		87.2
(0.2)
P 		69.1
(-1.8)
P 		103.2
(2)
P 		58.7
(-3)
P 		53
C 95.9
(-0.9)
P 		136.1
(0.8)
P 		104.4
(-0.6)
P 		185.1
(3)
P 		83.3
(-1.5)
P 		48.9
(-3)
P 		136
avr 108.7
(0.5) 		118
(1.1) 		100.1
(-0.2) 		143.3
(3) 		96.7
(-0.4) 		61.7
(-3) 		82
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 47
(3)
P 		44.5
(2.5)
P 		43.4
(2.3)
P 		14.8
(-3)
P 		32
B 48.2
(2.1)
P 		52.3
(3)
P 		38.2
(-0)
P 		24.5
(-3)
P 		28
C 57.5
(1.1)
P 		52.3
(0.2)
P 		68.3
(3)
P 		33.4
(-3)
P 		35
avr 50.9
(3) 		49.7
(2.7) 		50
(2.8) 		24.2
(-3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 24
(-1.8)
P 		37.8
(1.4)
P 		23.7
(-1.9)
P 		44.5
(3)
P 		31.3
(-0.1)
P 		18.9
(-3)
P 		26
B 34.8
(1.2)
P 		37.2
(1.8)
P 		21.2
(-2.3)
P 		18.3
(-3)
P 		41.9
(3)
P 		21.9
(-2.1)
P 		24
C 35.6
(1.6)
P 		41.5
(3)
P 		26.6
(-0.6)
P 		37.2
(2)
P 		26.9
(-0.5)
P 		16.4
(-3)
P 		25
avr 31.5
(0.8) 		38.8
(3) 		23.8
(-1.6) 		33.3
(1.3) 		33.4
(1.4) 		19.1
(-3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185495_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11271
Sequence Type Exemplar sequence
Representative Public ID C25E10.4 NCBI
Target Description C25E10.4 /REP_DB=WormBase Gene ID /WP=CE06866 /TR=Q18153 /GB=AAA92308.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072938(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072938 NCBI Caenorhabditis elegans C25E10.4 (C25E10.4) mRNA, complete cds. 11/11 None
SNAP00000038733 ENSEMBL cdna:SNAP chromosome:CEL140:V:9036279:9038046:1 11/11 None
GENEFINDER00000038749 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9033144:9038046:1 11/11 None
C25E10.4 ENSEMBL cdna:known chromosome:CEL140:V:9036180:9038192:1 gene:C25E10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9036279-9038047(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25E10.4
Entrez Gene 182889 Entrez gene
SwissProt Q18153 EMBL-EBI
WormBase CE36982 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA92308 Hypothetical protein C25E10.4 [Caenorhabditis elegans] ref|NP_505339.2| C25E10.4 [Caenorhabditis elegans] 0.0
blast CAE72079 Hypothetical protein CBG19165 [Caenorhabditis briggsae] 0.0
blast AAA92307 Hypothetical protein C25E10.5 [Caenorhabditis elegans] ref|NP_505338.1| C25E10.5 [Caenorhabditis elegans] 0.0
blast CAE72078 Hypothetical protein CBG19164 [Caenorhabditis briggsae] 0.0
blast AAA92307 Hypothetical protein C25E10.5 [Caenorhabditis elegans] ref|NP_505338.1| C25E10.5 [Caenorhabditis elegans] 1.0E-168
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505339.2 11 34-56,71-93,105-127,132-154,166-188,203-225,277-299,309-331,338-360,391-413,439-461
C25E10.4 11 34-56,71-93,105-127,132-154,166-188,203-225,277-299,309-331,338-360,391-413,439-461
SNAP00000038733 11 25-47,62-84,96-118,123-145,157-179,194-216,268-290,300-322,329-351,382-404,430-452
GENEFINDER00000038749 22 71-93,105-127,132-154,166-188,208-230,267-289,309-331,344-362,390-412,433-452,462-484,551-573,588-610,622-644,649-671,683-705,720-742,794-816,826-848,855-877,908-930,956-978
Sequence
Target Sequence 
>C. ELEGANS:185495_AT
gatagcttttggatttctggcatgctcaatacttctactctacattttctgccgtttagg
acaagtaataccacaagaatacgtattcttggcatgcacaattgctgcagggtcttactt
catcgtcacttatccattctcgtttaatagtcaaccacttgctccctataattcatcaac
acacgctggatgtaatccaaatgaatatacatggtgtgatacggcattggctgtggatcc
atgggttttcatgattactacagttattgtatttgctccatctttaccaatgatgggcac
atctttggatactgtgtattcaagagttcttggtgatattgatcaaaacattgcacacgg
gtttatgactattgtcgatgatgtcattttcatgattactccaattttaacaacatcaat
attcgcaatttttggagtaggtcctttatggattatgaaagctgttctgtttctttcaat
gggtgctctctggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATAGCTTTTGGATTTCTGGCATGC 23 425 918 Antisense
TTCTGGCATGCTCAATACTTCTACT 358 689 932 Antisense
CTACTCTACATTTTCTGCCGTTTAG 285 227 952 Antisense
GAATACGTATTCTTGGCATGCACAA 347 327 994 Antisense
CAATTGCTGCAGGGTCTTACTTCAT 389 179 1016 Antisense
TTACTTCATCGTCACTTATCCATTC 432 681 1032 Antisense
TTTAATAGTCAACCACTTGCTCCCT 376 663 1060 Antisense
GCTCCCTATAATTCATCAACACACG 508 299 1078 Antisense
GTGATACGGCATTGGCTGTGGATCC 25 485 1133 Antisense
GTATTTGCTCCATCTTTACCAATGA 676 453 1186 Antisense
TTTCTTTCAATGGGTGCTCTCTGGG 96 673 1387 Antisense
Sequence Source Affymetrix Proprietary Database